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Denitrification rhodococcus and rhodococcus microbial agent production method and application

A rhodococcus, functional technology, applied in the field of rhodococcus, can solve the problem of high emission cost

Active Publication Date: 2014-11-12
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

The cost of these waste water treatment to meet the discharge standards is high, and it is urgent to find a method for resource utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: monosodium glutamate waste water produces rhodococcus microbial bacterial agent

[0016] Pretreatment of monosodium glutamate wastewater: adjust the pH of the wastewater to 7.5 with lime water, and sterilize at 115°C for 20 minutes.

[0017] Liquid seed preparation: Inoculate rhodococcus preserved on a slant into a triangular flask containing pretreated monosodium glutamate waste water, vibrate and culture at 30° C. and 150 rpm for 30 hours to obtain liquid seeds.

[0018] Liquid fermentation: Inoculate 5% (volume ratio) liquid seeds into a fermentation tank equipped with pretreated MSG waste water, and cultivate for 30 hours at 32°C with sterile air; the cultured fermentation liquid is counted by plate bacteria, and the viable bacteria number up to 2.0×10 9 CFU / ml.

[0019] Bacterial agent preparation: use zeolite passing through a 80-mesh sieve as a carrier, add the fermentation broth to the biochar according to the weight ratio of fermentation broth:...

Embodiment 2

[0020] Embodiment 2: Rhodococcus microbial bacterial agent is applied to improve aquaculture water quality

[0021] The experimental base is located in the coastal area of ​​Guangdong. Four high-level culture ponds of Penaeus vannamei (each pond covers an area of ​​3 mu and the depth of the pond is 2.5 meters) are divided into two control ponds without adding bacterial agents and 2 treatment with bacterial agents. 360,000 shrimp seedlings were placed in each pond, and the breeding method was the same. Dilute the solid bacterial agent 20 times with water, mix it evenly, and evenly sprinkle it into the two treatment ponds according to the amount of 50 ml of dilution per cubic meter of water, once every 7 days in summer and once every 15 days in other seasons. The ammonia nitrogen content in the culture water of the treatment pond can be stably controlled below 0.5 mg / L, and the nitrite nitrogen can be controlled below 0.8 mg / L; while the ammonia nitrogen content in the culture w...

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Abstract

The invention relates to rhodococcus with the function of removal of ammonia nitrogen and nitrite in water and a low cost rhodococcus microbial agent production method and an application method, and belongs to the technical field of environmental microorganisms. The adopted strain is an efficient denitrification bacteria-(Rhodococcus sp.) YY12 strain separated and screened from activated sludge of sewage treatment plants, and the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No. 6924. According to the method, monosodium glutamate wastewater is used as a fermentation medium for large-scale culture of the Rhodococcus, the microbial agent for efficient removal of ammonia nitrogen and nitrite in water is prepared, and the process has the advantages of low production cost, simple operation, resource utilization of the monosodium glutamate wastewater, and reduction of pollution emissions. The microbial agent used for aquaculture not only can improve the water quality of aquaculture water, but also can significantly improve the aquatic products output, and shows a wide application prospect.

Description

Technical field: [0001] The invention relates to a rhodococcus strain capable of removing ammonia nitrogen and nitrite in water, and a low-cost production method and application method of the bacterial agent, belonging to the technical field of environmental microorganisms. Background technique: [0002] Excessive ammonia nitrogen content is one of the main factors of water pollution and eutrophication. The microbial denitrification method is widely recognized for its high efficiency, low cost, and no secondary pollution, and has become a research hotspot in the fields of environmental science and water treatment. However, there are still many problems in the traditional wastewater biological denitrification process. In recent years, the emergence of simultaneous nitrification and denitrification biological denitrification technology not only overcomes the problems existing in traditional biological denitrification, but also shortens the denitrification process, saves carbon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C02F3/34C12R1/01
Inventor 白志辉陈月月赵志瑞金德才庄绪亮庄国强
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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