Antagonistic bacterial strain for preventing and treating fruit tree rot and application thereof

A technology of rot disease and antagonistic bacteria, applied in the field of Bacillus pumilus, can solve the problems of destroying the ecological environment, incompleteness, and high cost of control, and achieves the effect of a wide range of adaptations

Active Publication Date: 2015-09-02
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and control of fruit tree rot is mainly based on chemical control, which is costly and incomplete. Long-term, repeated and large-scale use of chemical pesticides will cause soil, water and air pollution, increase pesticide residues, and food safety problems are becoming more and more serious; and Chemical pesticides destroy the ecological environment while killing pathogenic bacteria

Method used

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  • Antagonistic bacterial strain for preventing and treating fruit tree rot and application thereof
  • Antagonistic bacterial strain for preventing and treating fruit tree rot and application thereof
  • Antagonistic bacterial strain for preventing and treating fruit tree rot and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1 Isolation, screening and identification of antagonistic bacteria strain Bacillus pumilusXJAU-117

[0044] 1) Isolation of antagonistic bacteria strain Bacillus pumilusXJAU-117

[0045] The bacterial strain Bacillus pumilusXJAU-117 which antagonizes the fruit tree rot pathogen of the present invention is collected from the Aksu area of ​​Xinjiang. During the process of isolating the walnut tree rot pathogen, the conventional tissue separation method is adopted to sample from the border of the lesion at the junction of the disease and the health. Separated to get:

[0046] 1A) Select three 5×5mm plant tissue blocks with diseased spots, and disinfect the surface with 75% ethanol and 3% NaClO (put the tissue blocks in 75% ethanol, take them out quickly, and then place them in 3% NaClO for disinfection 5 min), and then washed 3 times with sterile water, blotted the water on the surface of the tissue block with sterilized filter paper, placed the 3 tissue blocks ev...

Embodiment 2

[0065] Example 2 Effect of antagonistic bacteria strain Bacillus pumilusXJAU-117 on the growth of rot pathogen

[0066] 1. Determination of the antibacterial rate of the antagonistic bacteria strain Bacillus pumilusXJAU-117 on the pathogenic bacteria of rot

[0067] The inhibitory effect of antagonistic bacteria strain Bacillus pumilusXJAU-117 on walnut rot pathogen, apricot rot pathogen, apple rot pathogen and poplar rot pathogen was detected by plate confrontation method. The assay method is the same as the method in step 2 in Example 1.

[0068] Walnut rot pathogen strains, apricot rot pathogen strains, apple rot pathogen strains, and poplar rot pathogen strains were provided by the Plant Pathology Laboratory of the College of Forestry and Horticulture, Xinjiang Agricultural University. The measurement results are as follows Figure 3-6 and shown in Table 2.

[0069] As shown in Table 2, the strain XJAU-117 has the highest inhibitory rate of 85.88% on walnut tree rot, an...

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Abstract

The invention discloses an antagonism bacterial strain preventing and treating fruit tree rot and the application of the antagonism bacterial strain. The antagonism bacterium is Bacillus pumilus XJAU-117, and the microbial preservation number is CGMCC No.9370. The Bacillus pumilus XJAU-117 is obtained from a rot branch of the walnut tree in a separation mode, the antagonism for the rot of the fruit tree is obvious and efficient, the rot pathogenic bacteria can be effectively prevented from growing, the plant disease caused by the pathogenic bacteria can be prevented and treated, and the antagonism bacterial strain is an efficient biological control potential bacterial strain with the good environmental safety and has good development and application prospects.

Description

technical field [0001] The invention relates to an antagonistic bacteria strain, in particular to a bacillus pumilus which antagonizes various pathogenic fungi of fruit tree rot disease and its application. Background technique [0002] Fruit tree rot is also known as rotten skin disease, stinky skin disease, etc., and it occurs all over the world. The rot disease mainly harms the branches and bark of the fruiting tree. When it is serious, it will cause the branches to wither, the fruiting ability will decrease, and the whole plant will die. Young trees and seedlings can also be killed, but the disease is less. In the early stage of the disease, it is not easy to identify from the outside. If you lift the skin of the branches, you can see dark brown to reddish-brown wet spots or yellowish-brown dry spots. Sometimes, the internal lesion area is already large, but it is still difficult to identify from the outside. . When the injury is severe, the cortex is rotten and necro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A61P3/00C12R1/07
Inventor 马荣朱银飞田呈明梁英梅李建贵王晓炜
Owner BEIJING FORESTRY UNIVERSITY
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