Tissue culture rapid propagation technology for ashitaba leaves

A technology of tissue culture and leaves, applied in the field of plant cultivation, can solve the problems of high price, insufficient raw materials, low germination rate of Ashitaba seeds, etc., and achieve the effect of low cost and wide source of materials

Inactive Publication Date: 2014-11-19
GUIZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a technology for rapid propagation of Ashitaba leaf tissue cultu...

Method used

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Embodiment Construction

[0018] The present invention will be described in detail below in combination with specific embodiments.

[0019] The present invention adopts MS as the base medium, and configures the combination of different plant growth substances:

[0020] Callus induction medium: MS+1mg / L6-BA+3mg / LNAA;

[0021] Callus proliferation medium: MS+6-BA2mg / L+2,4-D1mg / L;

[0022] Cluster bud induction medium: MS+6-BA1mg / L+NAA0.5mg / L;

[0023] Rooting induction: 1 / 2MS+0.02mg / LNAA.

[0024] The present invention carries out according to the following steps:

[0025] (1) Leaves are used as explants. The leaves are preferably upper and slightly young leaves, but avoid collecting new leaves. After washing with running water for 15-20 minutes, put them on a sterile operating table for later use.

[0026] (2) In the inoculation room, soak the plant material with 75% alcohol solution for 30 seconds on the ultra-clean workbench, take it out, rinse it with sterile water for 3 times, put it into 0.1% m...

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Abstract

The invention discloses a tissue culture rapid propagation technology for ashitaba leaves. The tissue culture rapid propagation technology comprises the following steps: selecting ashitaba leaves; washing with running water; soaking with a 75% alcohol solution; washing with sterile water; sterilizing in 0.1% L of a mercury solution and then washing with sterile water again; dicing; breeding in a callus induction medium, and culturing for 20-30 days so that a callus mass grows on a leaf cut; dicing the callus mass; transferring to a callus proliferation medium, culturing for 20-30 days and proliferating calluses; dicing the proliferated calluses; transferring to a cluster bud induction medium, culturing for 30 days, and differentiating to produce cluster buds; cutting the calluses on the roots of the cluster buds; splitting into single buds; inoculating a root medium with the buds and culturing for 20 days to obtain regeneration plant seedlings; and after the seedlings are domesticated for one week, transplanting into sterilized coconut shell land till the seedlings survive. The tissue culture rapid propagation technology has the beneficial effects that the cost is low and the material source is wide since the ashitaba leaves are used as propagation materials.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to the rapid propagation technology of ashitaba leaf tissue culture. Background technique [0002] Ashitaba is used for both medicine and food, especially in terms of medicinal value, and is recognized by all walks of life as a new vegetable with great development potential. Our country introduced Ashitaba from Japan more than 20 years ago. At present, there are only a few domestic companies that have just introduced Ashitaba. The cultivation area is very small, and the raw materials have high prices in the international market and a large demand. In the production process, Ashitaba plants usually enter the reproductive period 1-2 years after planting, and die immediately after flowering and fruiting. The flowering period of Ashitaba lasts for a long time, the fruit setting rate is low, and the maturity of the seeds varies during harvesting, and the fruit is double-hanging ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 罗充辛柯姚红艳冯晓英陈显刚吴涛罗开源赵敏
Owner GUIZHOU NORMAL UNIVERSITY
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