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A kind of kunlun savinol glycoside compound and its preparation method and application

A compound and related technology, applied in the field of extraction and separation of natural compounds, can solve problems such as carcinogenicity, and achieve the effect of strong DPPH and ABTS free radical scavenging ability

Active Publication Date: 2016-04-20
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the research and development of functional food is the frontier of food development in recent years and represents a trend of food development in the 21st century. However, some artificially synthesized food additives can produce toxic substances and are carcinogenic. It is of great significance to find natural active substances with high efficiency and no side effects for the production of functional food or medicine

Method used

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  • A kind of kunlun savinol glycoside compound and its preparation method and application
  • A kind of kunlun savinol glycoside compound and its preparation method and application
  • A kind of kunlun savinol glycoside compound and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the preparation of compound

[0031] Weigh 5 kg of dried Kunlun chrysanthemum capitulum, mash it with a homogenizer, put it in a 20L volumetric flask, add 10L methanol for extraction, leave it for 24 hours, and extract three times. Use a Buchner funnel to suction filter, place the filter residue in a 20L volumetric flask, and use 2mol·L -1 The NaOH solution was degraded in a water bath at 50°C for 2 hours, neutralized to neutral with hydrochloric acid, extracted with 10L n-butanol, left for 24 hours, and extracted three times. Extract the n-butanol layer, and use a rotary evaporator to concentrate in vacuo at 55° C. to obtain the n-butanol extract.

[0032] The n-butanol extract (152 g) was mixed with 100 g of silica gel in a dry method, then mounted on an atmospheric column equipped with 300 g of silica gel (300-400 mesh), and separated by petroleum ether-acetone-methanol gradient elution. The eluted components were concentrated in vacuum and then detec...

Embodiment 2

[0038] Embodiment 2, the test of antioxidant activity

[0039] 1. Experimental samples and experimental methods

[0040] 1.1 DPPH method.

[0041] Preparation of DPPH solution: Accurately weigh 0.0197g DPPH, adjust the volume to 250ml with absolute ethanol, and obtain a concentration of 2×10 -4 mol L -1 DPPH solution.

[0042] Determination method: Add 2ml DPPH solution to 2ml sample, shake well, keep it in the dark for 30min, measure the absorbance value A at 517nm I ; Replace the sample with 2ml of absolute ethanol for the blank, add 2ml of absolute ethanol to the 2ml sample, shake well and keep it in the dark for 30min, measure the absorbance value A at 517nm J ; Add 2ml of absolute ethanol to 2ml of DPPH solution, shake well, keep it in the dark for 30 minutes, and measure the absorbance A at 517nm C ; The clearance rate is [1-(A I -A J ) / A C ] × 100%. VE solution (20-100μmol L -1 ) as a positive control, and each sample was parallelized three times.

[0043] 1....

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Abstract

The invention discloses a Coreopsis tinctoria phenol glycoside compound, and a preparation method and application thereof. The Coreopsis tinctoria phenol glycoside compound is the compound disclosed as Formula (I) or pharmaceutically acceptable salt thereof. The 4,6-dihydroxy-2-[6-hydroxy-1,5-dimethyl-4-hexenyl]-5benzyl-O-beta-D-glucopyranoside separated from Coreopsis tinctoria is the compound obtained from natural resources for the first time; and the compound has strong capacity for clearing DPPH and ABTS free radicals, and can be used as a raw material, auxiliary material or adjuvant in the fields of production of food, medicines, cosmetics and the like.

Description

technical field [0001] The invention belongs to the field of extraction and separation of natural compounds, and in particular relates to a new Kunlun saiquiol glycoside compound, as well as its preparation method and application. Background technique [0002] Kunlun snow chrysanthemum, also known as blood chrysanthemum, scientific name Coreopsistinctoria Nutt, is a rare alpine Compositae plant with unique efficacy, mainly distributed in some areas above the snow line at an altitude of 3,000 meters at the northern foot of the Kunlun Mountains. Studies have confirmed that Kunlun Snow Chrysanthemum has the effects of lowering blood pressure, blood lipids, blood sugar, antibacterial, and anti-inflammatory; in vitro activity studies have shown that Kunlun Snow Chrysanthemum extract has significant antioxidant and anti-tumor activities. [0003] Phenolic glycosides are a kind of complex secondary metabolites of polyphenols that widely exist in plants. Studies have found that plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08A61P39/06A61Q19/00A23L33/125
Inventor 辛志宏陈伟杨英士张俊楠郭佳王伟
Owner NANJING AGRICULTURAL UNIVERSITY