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Peptide bifonctionnel

A bifunctional peptide and sequence technology, applied in the direction of peptides, specific peptides, fusion polypeptides, etc., can solve the problem of not reducing the expression of enzymes

Active Publication Date: 2014-11-19
REGENTIS INT +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] However, the peptides described in these patent documents have the same drawbacks as exemplified above, namely, the fact that the sequences proposed in these patent documents cannot reduce the expression of enzymes leading to the degradation of proteins of the extracellular matrix, and in particular cannot reduce Expression of enzymes leading to collagen degradation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Example 1: Effects of Bifunctional Peptides on Collagen Synthesis-Protein Analysis

[0117] Dermal fibroblasts were isolated from abdominal skin biopsies obtained during surgical procedures in healthy patients between the ages of 35 and 75.

[0118] After removing the epidermis, fibroblasts were recovered by enzymatic digestion, followed by culture at 37°C in DMEM (Dulbecco's Modified Eagle's Medium) supplemented with 10% fetal bovine serum and 1% penicillin / streptomycin .

[0119] Next, the fibroblasts were treated with a peptide having the S1 sequence or fragments thereof at a concentration of 100 μg / ml. The various fragments tested are as follows:

[0120] - Peptide 1 (pept1): peptide with S1 sequence

[0121] - Peptide 2 (pept2): Sequence N-(Val-Gly-Val-Ala-Pro-Gly) 3 Arg-Val-Arg-Leu-OH

[0122] - Peptide 3 (pept3): N-(Val-Gly-Val-Ala-Pro-Gly) 3 -OH

[0123] - Peptide 4 (pept4): N-Arg-Val-Arg-Leu-Gly-Ile-Leu-OH

[0124] - Peptide 5 (pept5): N-Gly-Ile-Leu-O...

Embodiment 2

[0130] Example 2 : Effect of Bifunctional Peptides on Collagen Synthesis - Gene Expression Analysis

[0131]Gene expression of collagen type I and type III in fibroblast cultures was tested in the presence of different peptides (pept1 to pept5) at a concentration of 100 μg / ml.

[0132] After 24 hours of treatment with different peptides, using Kit (QIAGEN) RNAs were extracted from the culture, followed by the use from SABiosciences TM The RT2 First Strand Kit (RT2First Strand Kit) retranscribes RNAs into complementary DNAs (cDNAs).

[0133] Next, real-time PCR analysis was performed in a 96-well plate using a Mx3000p thermal cycler from Stratagene. Each sample was analyzed in triplicate. cDNAs containing RT2 Green / ROX TM qPCR Master Mix reagents (SABiosciences TM ) and sense and antisense primers specific for the gene to be amplified are used as substrates in the reaction mixture. Fluorescence was read at the end of each cycle and the readings were analyzed by MxPr...

Embodiment 3

[0137] Example 3 : Effect of Bifunctional Peptides on Collagen Synthesis - Protein and Gene Expression Tests According to Patient Age

[0138] Dermal fibroblasts from younger or older patients (35, 42 and 53 years old) were cultured and the peptides of the invention having the S1 sequence were tested for protein and gene expression of type I and type III collagen. Figure 5 The results obtained for the protein expression of the two types of collagen can be seen. It can be seen that the effect of the peptides of the present invention on the synthesis of collagen is greater when the fibroblasts are from older patients.

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Abstract

The present invention concerns a bifunctional peptide capable of activating collagen synthesis and inhibiting the production of matrix metalloproteinases, said peptide having a sequence comprising three peptide parts A, B and C: - the first peptide part A corresponding to a hexapeptide repeated at least three times, said part A being capable of bonding to a receptor elastin-binding protein in order to stimulate collagen synthesis, the second peptide part B corresponding to a tetrapeptide capable of acting as a competitive inhibitor of urokinase protease and of being cleaved by said protease, and, the third peptide part C corresponding to a tripeptide occupying at least one active site of the matrix metalloproteinases in order to enable inhibition of said proteinases. The present invention further concerns a cosmetic and / or pharmaceutical composition incorporating the bifunctional peptide.

Description

technical field [0001] The present invention relates to a peptide that will be used in the field of cosmetics and medicine. [0002] The present invention will mainly be applied in the field of repair or regeneration of dermal tissue, especially affected by skin aging. Background technique [0003] Skin aging is more or less due to a large-scale imbalance of synthetic control mechanisms and degradation of components of the extracellular matrix of the dermis, which constitutes the innermost layer of the skin. Thus, during skin aging, in the extracellular matrix of the dermis, a decrease in the synthesis of certain macromolecules (i.e. collagen and elastin) on the one hand and certain enzymes (e.g. Increased expression of metalloproteinases or MMPs). The latter exhibit collagenolytic and elastin dissociative activity and thus lead to the degradation of macromolecules of the dermal matrix. As a result, the skin loses its tone and the epidermis expands, leading to the formati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/55A61Q19/00A61Q19/08C07K4/12
CPCC07K14/78C07K2319/50A61K38/00C07K5/1019A61K8/64A61Q19/08C07K2319/00C07K4/12C07K5/0804C07K2319/75A61P17/00A61P17/02C07K14/8146
Inventor 威廉·霍恩贝克琼·阿蒂亚桑德里纳·洛里默弗兰克·安东尼斯利
Owner REGENTIS INT