Application of bone marrow mesenchymal stem cells-derived neuron cells as medicine for treating n-hexane induced peripheral neuropathy

A technology of bone marrow mesenchyme and nerve cells, applied in the specific application field of nerve cells

Inactive Publication Date: 2014-11-26
DALIAN MEDICAL UNIVERSITY
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  • Application Information

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Problems solved by technology

At present, the treatment of patients with chronic n-hexane poisoning includes breaking away from contact, ensuring that patients have adequate nutrition, giving B vitamins, energy mixtures, promoting blood circulation and removing blood stasis, Chinese medicine for dredging collaterals and tonifying kidneys, and supplemented by acupuncture, physical therapy, and limb exercise. There is no specific drug treatment

Method used

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  • Application of bone marrow mesenchymal stem cells-derived neuron cells as medicine for treating n-hexane induced peripheral neuropathy
  • Application of bone marrow mesenchymal stem cells-derived neuron cells as medicine for treating n-hexane induced peripheral neuropathy
  • Application of bone marrow mesenchymal stem cells-derived neuron cells as medicine for treating n-hexane induced peripheral neuropathy

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Experimental program
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Embodiment Construction

[0019] Isolation and culture of bone marrow mesenchymal stem cells (MSCs):

[0020] The rats were killed by dislocation, immersed in 75% ethanol for disinfection, aseptically stripped the femur and tibia, removed the metaphysis, exposed the bone marrow cavity, washed repeatedly with PBS, collected the cells, passed through the sieve, centrifuged, and reconstituted with 10% serum-containing DMEM medium Suspend the cells and inoculate them in a petri dish, at 37 ℃, CO 2 Cultivate in a saturated humidity incubator, change the medium after 24 hours. After 3 days, the medium was changed once, and when the cells reached 80% to 90% confluence, the MSCs were gradually purified by the differential adhesion method. Take the 3rd generation cells with good growth status and use the monoclonal antibodies CD90 and CD45 for flow cytometry to identify MSCs.

[0021] Preparation and identification of nerve cells (mNCs) derived from bone marrow mesenchymal stem cells:

[0022] When the growth densit...

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Abstract

The invention relates to a concrete application of bone marrow mesenchymal stem cells-derived neuron cells (mNCs), solves a problem that no medicines for specially effectively treating n-hexane induced peripheral neuropathy traditionally exist, and provides a new idea for treating the neuropathy and the concrete application of the neuron cells. Results of experiments with rats suffering from the n-hexane induced peripheral neuropathy as an experiment object and gait score, nerve electrophysiology (nerve conduction latency and nerve conduction velocity), and solochrome cyanine staining, scanning electron microscope, myelin basic proteins and low molecular weight neurofilaments of spinal marrow and sciatic nerves as detection indexes show that the mNCs has a substantial restoration effect on the rats suffering from the n-hexane induced peripheral neuropathy as the medicine for treating n-hexane induced peripheral neuropathy, and has important effects in the treatment of occupational poisoning induced peripheral neuropathy.

Description

technical field [0001] The invention relates to a specific application of nerve cells, in particular to the application of nerve cells derived from bone marrow mesenchymal stem cells as a drug for treating peripheral neuropathy induced by n-hexane. Background technique [0002] The nervous system damage in patients with chronic n-hexane poisoning (n-hexane-induced peripheral neuropathy) is caused by the final metabolite of n-hexane and the final poison 2, 5-hexanedione (HD). Its pathological features are axonal neurofilament accumulation and segmental swelling, followed by segmental demyelination, especially long and thick fibers. Many scholars have studied the pathogenesis of n-hexane toxic peripheral neuropathy from the perspectives of axonal skeleton proteins, energy metabolism disorders, and NGF signal transduction pathways, but so far it has not been fully understood. At present, the treatment of patients with chronic n-hexane poisoning includes breaking away from cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P25/02
Inventor 朴丰源李双月王哲敏董伟陈若霖戚媛刘爽
Owner DALIAN MEDICAL UNIVERSITY
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