A Molecular Marker Closely Linked to the Soybean Rust Resistance Gene Locus and Its Application

A soybean anti-rust and anti-rust technology, which is applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effects of clear location, saving production costs, and reliable use

Active Publication Date: 2015-10-28
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the Soybean Institute of the Brazilian Institute of Agriculture and Livestock Research, rust control cost up to US$ 2 billion per production season in 2009 / 2010, in addition to yield losses

Method used

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  • A Molecular Marker Closely Linked to the Soybean Rust Resistance Gene Locus and Its Application
  • A Molecular Marker Closely Linked to the Soybean Rust Resistance Gene Locus and Its Application
  • A Molecular Marker Closely Linked to the Soybean Rust Resistance Gene Locus and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Using the primer pair GmSSR18-22 to identify the F of Tianlong No. 1 × SX6907 2 Individual plant resistance to rust

[0036] 1. PCR reagents for identification or auxiliary identification of soybean rust resistance

[0037] The PCR reagent for identification or auxiliary identification of soybean rust resistance in this embodiment consists of PCR primer pair GmSSR18-22, 10×Taq buffer, dNTP mixture, MgCl 2 solution, Taq DNA polymerase and ddH 2 O composition.

[0038] Among them, the PCR primer pair GmSSR18-22 consists of two single-stranded DNAs, a forward primer and a reverse primer, and its sequence is as follows:

[0039] Forward primer: 5'-ACCAAACCCGATGATGATGT-3' (sequence 1 in the sequence listing),

[0040] Reverse primer: 5'-CCAGATTCCAAACCCCTTCT-3' (sequence 2 in the sequence listing).

[0041]2. Soybeans to be identified

[0042] F of Tianlong No. 1 × SX6907 2 The segregation population is obtained as follows: Tianlong No. 1 and SX6907 are cros...

Embodiment 2

[0108] Embodiment 2, the acquisition of primer pair GmSSR18-22

[0109] The segregating populations used were non-rust resistant soybean variety Zhongdou 40 (Zhang Cheng, introducing six nationally approved soybean varieties, Farm Consultant, 2012, 4:35-37) and rust resistant soybean SX6907 (Shan Zhihui et al., a soybean rust Screening and identification of new resistance sources, Chinese Journal of Oil Crops, 2012, 34: 188-192) hybridization, hybrid offspring self-crossing, namely F 2 A total of 116 individual plants were isolated from the population.

[0110] to F 2 The 116 individual plants were analyzed by SSR molecular markers to obtain genotype data, and the genetic map was constructed using Joinmap 3.0, combined with the identification data of rust resistance inoculation, and the rust resistance gene was mapped using WinQTL cartographer 2.5, and the soybean resistance to rust was mapped on soybean chromosome 18. The rust gene locus rpp.18-1 belongs to complete dominan...

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Abstract

The invention discloses a molecular marker in close linkage with soybean rust resistance gene loci and application. The method for identifying soybean rust resistance or identifying soybean rust resistance in an assisted mode provided by the invention comprises the following steps: respectively by taking genome DNA of soybeans to be identified and SX6907 as a template, performing PCR amplification by using a PCR primer pair consisting of two single-strand DNAs shown in a sequence 1 and a sequence 2 in a sequence table, detecting the amplification product through electrophoresis, and determining the disease resistance of the soybeans to be identified according to the standard. A genetic group is established by hybridizing a high-rust resistance soybean SX6907 material with two non-rust resistance soybean materials, the rust resistance genes are located, the molecular marker in close linkage is developed, molecular marker-assisted selection is performed, the selection efficiency can be improved, and the breeding process is accelerated.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology and genetic breeding. More specifically, it relates to a molecular marker closely linked with the soybean rust resistance gene locus and its application, and also relates to the application of the molecular marker in soybean rust resistance breeding. Background technique [0002] Soybeans originated in China and are widely grown in countries such as the United States, Brazil, Argentina, China and India. Containing about 40% protein and 20% oil in soybean seeds, it is an oil crop with important economic value and the main source of plant protein. In recent years, soybean planting area and yield have declined in my country, among which soybean rust is one of the factors affecting soybean yield, mainly affecting the central and southern production areas of my country, resulting in yield losses ranging from 10% to 90%. The average yield of soybeans in the world has not increased, m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 单志慧陈海峰杨中路沙爱华邱德珍张婵娟陈李淼袁松丽张晓娟赵胜陈水莲万乔周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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