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Low-cost low-acyl transparent type gellan gum extraction process

An extraction process, low acyl technology, applied in the field of low acyl transparent gellan gum extraction process, can solve the problems of high cost, large acid-base changes, product quality damage, etc., to achieve low product damage, small pH changes, The effect of avoiding pollution

Active Publication Date: 2014-12-10
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of the traditional process of extracting gellan gum, such as high cost, excessive acid-base changes during the extraction process, high temperature in the gel stage and damage to product quality, the present invention provides a new low-acyl transparent type gellan gum method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A low-acyl transparent gellan gum extraction process with low cost, the process comprises the following steps:

[0033] (1) Enzyme treatment:

[0034] Add lysozyme at a concentration of 150mg / L (enzyme activity is 200,000 U / g) and acid protease at a concentration of 100mg / L (enzyme activity is 100,000 U / g) to the fermentation broth in turn, and keep warm at 60°C for 5h to carry out enzyme solution, then filter to remove the bacteria and protein impurities in the fermentation broth, and collect the filtrate for subsequent use;

[0035] (2) centrifugal impurity removal:

[0036] Adjust the filtrate to neutrality, then centrifuge at 1000 rpm for 5 minutes to remove the soluble impurities in the upper layer and collect the fibrous material in the lower layer;

[0037] (3) Dissolving:

[0038] Add 25 times the weight of deionized water to the fibrous material obtained in step (2), and heat up to 95° C., and keep the temperature for 3 minutes;

[0039] (4) Deacylation:

...

Embodiment 2

[0050] A low-acyl transparent gellan gum extraction process with low cost, the process comprises the following steps:

[0051] (1) Enzyme treatment:

[0052] Add 200mg / L concentration of lysozyme (enzyme activity is 200,000 U / g) and 120mg / L concentration of acid protease (enzyme activity is 100,000 U / g) to the fermentation broth in turn, keep it warm at 65°C for 5h, and carry out enzyme solution, then filter to remove the bacteria and protein impurities in the fermentation broth, and collect the filtrate for subsequent use;

[0053] (2) centrifugal impurity removal:

[0054]Adjust the filtrate to neutrality, then centrifuge at 1000 rpm for 6 minutes to remove the soluble impurities in the upper layer and collect the fibrous material in the lower layer;

[0055] (3) Dissolving:

[0056] Add 25 times the weight of deionized water to the fibrous material obtained in step (2), and heat up to 95° C., and keep the temperature for 4 minutes;

[0057] (4) Deacylation:

[0058] Ad...

Embodiment 3

[0071] The performance parameter of the low acyl gellan gum prepared by embodiment 1 and embodiment 2:

[0072] Determination of molecular weight (determination of acyl group content in gellan gum by acid-base titration): the average molecular weight of the gellan gum prepared in Example 1 and Example 2 is about 740,000 Daltons.

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Abstract

The invention relates to a low-cost low-acyl transparent type gellan gum extraction process which specifically comprises the following steps: (1) performing enzyme treatment; (2) performing centrifugal decontamination; (3) dissolving; (4) performing deacylation; (5) filtering; (6) chelating; (7) performing solid-liquid separation; (8) performing drying and crushing; and (9) treating wastes. The low-cost low-acyl transparent type gellan gum extraction process is gentle in process conditions, the pH value is relatively small in fluctuation in product treatment, no polyvalent metal ion is used in the process, and meanwhile due to a proper amount of chelating agent, a good dissolving property of a product is ensured; according to the process, no lower alcohol is adopted for washing gellan fiber, so that the production cost is greatly lowered, the treatment conditions are gentle, and the product quality is improved.

Description

technical field [0001] The invention belongs to the field of microbial polysaccharide gellan gum production, and in particular relates to a low-cost low-acyl transparent gellan gum extraction process. Background technique [0002] Gellan gum is a high-molecular biological polysaccharide substance composed of repeated four sugar molecules connected by D-glucose, D-glucuronic acid, D-glucose, and L-rhamnose through glycosidic bonds. Sphingomonas paucimobilis is obtained by aerobic fermentation of fermenting bacteria. Gellan gum has good gel performance and is also an excellent thickener and stabilizer. Gellan gum is easy to use, has good flavor release, high thermal stability, easy to melt in the mouth, high transparency, gel time and temperature can be adjusted, the gel is not easily affected by pH, the product is stable, and has various flavors. Texture characteristics, it is one of the superior bio-glues at present, and has a wide range of applications. In the field of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 肖勇李学朋沈伟伟董爽
Owner XINJIANG FUFENG BIOTECH
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