Ultra-high pressure cell disruptor

Abstract

The invention discloses an ultra-high pressure cell crusher which comprises a case, wherein the case is internally provided with a circulation water bath tank and an accommodating chamber, wherein a pressurization and homogenization integrated device is mounted inside the circulation water bath tank; the pressurization and homogenization integrated device consists of a long oil cylinder, a main connecting sleeve, a high pressure cylinder homogenization main body, a secondary connecting sleeve and a short oil cylinder which are mounted in sequence on a same axis; a straight line type hydraulic station consisting of an oil tank, a hydraulic integrated block, an oil pump and a motor which are connected in sequence on a same axis is mounted inside the accommodating chamber; the axis of the straight line type hydraulic station is parallel to that of the pressurization and homogenization integrated device. The ultra-high voltage cell crusher disclosed by the invention is compact in structure and small in size, a small space is occupied, a small amount of connecting tubes are used, and due to a small amount of procedures, liquids are not easy to leak off.

Description

technical field [0001] The invention relates to a cell disruptor, in particular to an ultra-high pressure cell disruptor. Background technique [0002] Cell disruption technology refers to the technology of using external force to destroy the cell membrane and cell wall to release the cell content including the target product components. It is the basis for the separation and purification of non-secreted biochemical substances (products) synthesized in cells. Combining major advances in recombinant DNA technology and tissue culture technology, proteins previously thought to be difficult to obtain can now be produced on a large scale. A variety of cell disruption methods have been developed to accommodate different uses and different types of cell wall disruption. The crushing methods can be divided into two categories: mechanical method and non-mechanical method. There are also many kinds of equipment used in the mechanical method, and the commonly used equipment is ultra-...

AI Technical Summary

Problems solved by technology

The disadvantages of this commonly used ultra-high pressure cell crushing device are: (1) it adopts the crank mechanism to drive, the high pressure state cannot stop and start, it cannot run without load, it cannot be immersed in a circulating water bath, the temperature rises, and it cannot run continuously for a long time, There are many connecting links and it is easy to leak; (2) the liquid outlet of the pressurized homogenizer is opened at the upper part, and the liquid is not smooth, and there is easy residue; (3) the pressure gauge of the pressurized homogenizer is directly connected to the high-pressure chamber , making the high-pressure gauge pulsate frequently, which is easy to damage; (4) The high-pressure homogenizing valve is easy to wear

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