Bacillus subtilis

A Bacillus subtilis, preservation number technology, applied in the field of bioengineering, can solve the problems of slow degradation of soil organic phosphorus, long duration of treatment process, etc.

Active Publication Date: 2014-12-10
北京中晟丰和微生物技术有限公司
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Problems solved by technology

Soil microbial remediation technology has been greatly developed in foreign countries in the past ten years, but the degradation rate of soil organic phosphorus is slow, resulting in a long treatment process, which has always been a prominent difficulty in this technology. Therefore, it is necessary to look for efficient organic phosphorus degradation Bacteria can convert organic phosphorus, which is difficult for plants to absorb and utilize in soil, into a form that can be absorbed and utilized, which is currently a research hotspot in microbial remediation technology of phosphorus-contaminated soil

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  • Bacillus subtilis

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Embodiment Construction

[0029] Screening of strains:

[0030] Step 1. Enrichment: Collect about 100g of soil samples from the fig orchard of the Biotechnology College of Xi'an University of Arts and Sciences in Yanta District, Xi'an City. Aseptically weigh 10.0 g of the soil sample and put it into a 250 mL Erlenmeyer flask containing 100.0 mL of enrichment medium (sterilized), and enrich and cultivate it at 30°C and 140 r / min on a constant temperature shaker for 5 days; then absorb 5 mL of culture medium The solution was re-transferred into a fresh enrichment medium, and enrichment culture was carried out 3 times, each time for 5 days; the composition of the enrichment medium was: glucose 3.506g, peptone 0.83g, yeast extract 0.5g, diphosphate Potassium hydrogen 0.35g, calcium carbonate 0.25g, lecithin 0.02g, distilled water 1000mL, medium pH 6.8-7.1, sterilized at 105KPa for 20min-25min;

[0031] Step 2, screening and purification: Dilute the enriched culture solution after 3 enrichment cultures in ...

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Abstract

The invention discloses a Bacillus subtilis ZK1-18 of which the collection number is CGMCC No.9402. The Bacillus subtilis ZK1-18 strain is derived from soil, and has the advantages of high ecological safety, no corrosion and no harm to animals and plants. The strain is a dominant strain screened on the basis of the organic phosphorus source, and can change the properties of the organic phosphorus in the growth and reproduction process so as to change the organic phosphorus components, thereby performing the phosphate-solubilizing function. The strain provides an efficient and stable strain and base data for subsequent development of biological organic fertilizers capable of degrading organic phosphorus in soil.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a bacillus subtilis. Background technique [0002] Phosphorus is a major nutrient element for plant growth. It mainly exists in the form of insoluble minerals in the soil, which leads to a low utilization rate of phosphorus in the soil by plants and affects the growth of plants. The research on phosphate-solubilizing bacteria in my country started in the 1950s. Phosphorus-solubilizing bacteria can convert insoluble or insoluble phosphorus that is difficult for plants to absorb and utilize into usable forms, improve the utilization efficiency of phosphorus in soil, and reduce the application of chemical fertilizers. , to reduce the cost of agricultural input, so the microbial remediation of the soil environment is one of the important ways to increase crop yield and solve the lack of available phosphorus in the soil. However, the research on phosphate-solubilizi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 赵咏梅
Owner 北京中晟丰和微生物技术有限公司
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