Method for preparing high-activity collagen peptide from animal placentae and prepared high-activity collagen peptide and application of high-activity collagen peptide
A technology of collagen peptides and collagen, which is applied in the fields of protein biochemical engineering and medicine, can solve the problems of high degree of collagen denaturation, low effect, and failure to meet the objective needs of collagen, and achieve high collagen activity, simple operation, The effect is cheap to produce
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0083] After the cow gave birth, take 10kg of frozen placenta, chop it up, add 50 liters of tap water, soak overnight, and wash it repeatedly with clean water 3 times the next day to fully remove the blood, grind it with a colloid mill, add 200 liters of 3% acetic acid, and stir and extract overnight. Centrifuge at 14,000 rpm for 40 minutes to collect the precipitate. Add 30 liters of deionized water to the precipitate, stir and mix well, adjust the pH to 8.6, add 30 grams of trypsin, and incubate at 42°C for 8 hours. Filter with a 0.2-micron membrane basket filter, concentrate the passing liquid to 10 liters with a nanofiltration machine, then add deionized water to 30 liters, stir and concentrate by nanofiltration, repeat 4 times, finally concentrate to 10 liters, and freeze-dry , to get 0.3 kg of collagen peptides.
Embodiment 2
[0085] After the cow gave birth, take 100kg of fresh frozen placenta, chop it up, add 500 liters of tap water, soak it overnight, rinse it with clean water 3 times the next day, fully remove the blood, grind it with a colloid mill, add 2000 liters of 3% acetic acid, stir and extract it overnight . Centrifuge at 14,000 rpm for 50 minutes to collect the precipitate. Add 300 liters of deionized water to the precipitate, stir and mix well, adjust the pH to 8.6, add 300 grams of trypsin, and incubate at 42°C for 8 hours. Filter with a 0.2-micron membrane basket filter, concentrate the passing liquid to 100 liters with a nanofiltration machine, then add deionized water to 30 liters, stir and concentrate by nanofiltration, repeat 4 times, finally concentrate to 100 liters, and freeze-dry , get 3.5 kg of collagen peptides.
Embodiment 3
[0087] After giving birth, take 500kg of fresh frozen placenta, chop it up, add 2500 liters of tap water, soak it overnight, rinse it with clean water 3 times the next day, fully remove the blood, use a colloid mill to refine it, add 10000 liters of 3% acetic acid, stir and extract overnight . Centrifuge at 14,000 rpm for 60 minutes to collect the precipitate. Add 1,500 liters of deionized water to the precipitate, stir and mix, adjust the pH to 8.6, add 3,000 grams of trypsin, and incubate at 42°C for 8 hours. Filter with a 0.2-micron membrane basket filter, concentrate the liquid through a nanofiltration machine to 2,500 liters, then add deionized water to 15,000 liters, stir and concentrate by nanofiltration, repeat 4 times, and finally concentrate to 400 liters, freeze-dry , get 20 kg of collagen peptides.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com