Method for preparing high-activity collagen peptide from animal placentae and prepared high-activity collagen peptide and application of high-activity collagen peptide

A technology of collagen peptides and collagen, which is applied in the fields of protein biochemical engineering and medicine, can solve the problems of high degree of collagen denaturation, low effect, and failure to meet the objective needs of collagen, and achieve high collagen activity, simple operation, The effect is cheap to produce

Active Publication Date: 2014-12-10
吉林金梓源生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Products using this collagen are less effective in actual application in beauty and health care products
Unable to satisfy the world's objective demand for collagen
[0007] Based on solvin

Method used

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  • Method for preparing high-activity collagen peptide from animal placentae and prepared high-activity collagen peptide and application of high-activity collagen peptide
  • Method for preparing high-activity collagen peptide from animal placentae and prepared high-activity collagen peptide and application of high-activity collagen peptide
  • Method for preparing high-activity collagen peptide from animal placentae and prepared high-activity collagen peptide and application of high-activity collagen peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] After the cow gave birth, take 10kg of frozen placenta, chop it up, add 50 liters of tap water, soak overnight, and wash it repeatedly with clean water 3 times the next day to fully remove the blood, grind it with a colloid mill, add 200 liters of 3% acetic acid, and stir and extract overnight. Centrifuge at 14,000 rpm for 40 minutes to collect the precipitate. Add 30 liters of deionized water to the precipitate, stir and mix well, adjust the pH to 8.6, add 30 grams of trypsin, and incubate at 42°C for 8 hours. Filter with a 0.2-micron membrane basket filter, concentrate the passing liquid to 10 liters with a nanofiltration machine, then add deionized water to 30 liters, stir and concentrate by nanofiltration, repeat 4 times, finally concentrate to 10 liters, and freeze-dry , to get 0.3 kg of collagen peptides.

Embodiment 2

[0085] After the cow gave birth, take 100kg of fresh frozen placenta, chop it up, add 500 liters of tap water, soak it overnight, rinse it with clean water 3 times the next day, fully remove the blood, grind it with a colloid mill, add 2000 liters of 3% acetic acid, stir and extract it overnight . Centrifuge at 14,000 rpm for 50 minutes to collect the precipitate. Add 300 liters of deionized water to the precipitate, stir and mix well, adjust the pH to 8.6, add 300 grams of trypsin, and incubate at 42°C for 8 hours. Filter with a 0.2-micron membrane basket filter, concentrate the passing liquid to 100 liters with a nanofiltration machine, then add deionized water to 30 liters, stir and concentrate by nanofiltration, repeat 4 times, finally concentrate to 100 liters, and freeze-dry , get 3.5 kg of collagen peptides.

Embodiment 3

[0087] After giving birth, take 500kg of fresh frozen placenta, chop it up, add 2500 liters of tap water, soak it overnight, rinse it with clean water 3 times the next day, fully remove the blood, use a colloid mill to refine it, add 10000 liters of 3% acetic acid, stir and extract overnight . Centrifuge at 14,000 rpm for 60 minutes to collect the precipitate. Add 1,500 liters of deionized water to the precipitate, stir and mix, adjust the pH to 8.6, add 3,000 grams of trypsin, and incubate at 42°C for 8 hours. Filter with a 0.2-micron membrane basket filter, concentrate the liquid through a nanofiltration machine to 2,500 liters, then add deionized water to 15,000 liters, stir and concentrate by nanofiltration, repeat 4 times, and finally concentrate to 400 liters, freeze-dry , get 20 kg of collagen peptides.

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Abstract

The invention discloses a novel method for preparing high-activity collagen peptide from cow placentae. The method comprises the following steps: maintaining mammal placentae at a low temperature, chopping, soaking and rinsing, grinding into pulp, extracting, centrifuging, performing enzymolysis, filtering, desalting, concentrating and freeze-drying. The method disclosed by the invention has the characteristics that the extraction effect is good, the high-activity collagen peptide prepared by using the method is high in purity, the activity is high, a product containing the collagen peptide is easy to absorb, and the like. The method can be simply operated, is low in production cost and is applicable to on-scale industrialization production, and the purpose that the high-activity collagen peptide is efficiently prepared from cow placentae is achieved.

Description

technical field [0001] The invention relates to a method for preparing highly active collagen peptides from bovine placenta, belonging to the fields of protein biochemical engineering and medicine. Background technique [0002] The following description facilitates the reader's understanding. None of the information provided or references cited is admitted to be prior art to the present invention. [0003] Placenta (placenta), also known as "Ziheche", is a commonly used traditional Chinese medicine material, and it is often boiled and eaten by the people. my country's traditional Chinese medicine theory believes that: placenta is sweet and salty in nature, belongs to the lung, liver, and kidney meridian, and has the functions of nourishing qi, nourishing blood, and benefiting essence, and can be used as a nourishing, beautifying, and strong medicine. Modern biological and medical research has proved that the placenta is rich in collagen in addition to a variety of immunogl...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78A61K38/39A61K8/65A61Q19/02A61Q19/08A61Q19/00A23L1/305A61P39/06A23L33/18
Inventor 刘爽庞海庞玉邓小霞
Owner 吉林金梓源生物科技股份有限公司
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