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Method for determining content of paeoniflorin in flavored motherwort cream

A technology of flavored motherwort cream and a determination method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high detection accuracy and low detection limit, and achieve good precision and repeatability, high measurement accuracy, The effect of high detection sensitivity

Active Publication Date: 2014-12-10
上海海虹实业(集团)巢湖今辰药业有限公司
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Problems solved by technology

In the prior art, the content determination of paeoniflorin is mainly realized by thin-layer scanning method and high performance liquid chromatography, but for the component-specific flavored motherwort cream, the content determination method of paeoniflorin still needs to be improved. To achieve lower detection limits and higher detection accuracy

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  • Method for determining content of paeoniflorin in flavored motherwort cream
  • Method for determining content of paeoniflorin in flavored motherwort cream
  • Method for determining content of paeoniflorin in flavored motherwort cream

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Embodiment Construction

[0017] The content of the present invention will be described below in conjunction with specific embodiments.

[0018] The method for determining the content of paeoniflorin in the modified motherwort ointment of the present invention is as follows:

[0019] (1) Instruments and reagents: Waters 510 pump, Waters 486 UV-visible detector, Rheodyne sampler, WDL-95 chromatographic workstation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, water is purified water, and trifluoroacetic acid is chromatographically pure (TEDIA) .

[0020] (2) Reference substance source and purity inspection: Paeoniflorin reference substance is provided by the National Institute for the Control of Pharmaceutical and Biological Products, batch number: 110736-200423.

[0021] (3) Selection of chromatographic conditions: Chromatographic column: Hanbang Technology Co., Ltd., Kromasil C18 (5μ, 4.6×250mm); mobile phase: a mixture of methanol with a volume ratio of 24 to 76 and a trifluoroacetic ac...

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Abstract

The invention discloses a method for determining the content of paeoniflorin in flavored motherwort cream. The method adopts a high-performance liquid chromatography to determine the content of paeoniflorin in the flavored motherwort cream, and a sample pretreatment method comprises the following steps: precisely weighing 8-15g of flavored motherwort cream, and adding 15-30ml of methanol solution; carrying out ultrasonic treatment for 40 minutes, cooling to the room temperature, metering the volume to 50ml with methanol, shaking up, centrifuging for 15 minutes, and taking supernatant fluid. Chromatographic conditions are as follows: a Kromasil C18 chromatographic column is used, a mobile phase is an aqueous solution of methanol and 0.1% trifluoroacetic acid (the volume ratio is 24 to 76), the flow speed of mobile phase is 1.0 ml / min, the determining wavelength is 231nm, and the column temperature is 30.0 DEG C. The method aims at the flavored motherwort cream with special ingredients and can accurately determine the paeoniflorin with low content so as to be beneficial to accurately controlling the feeding amount of radix paeoniae alba.

Description

Technical field [0001] The invention relates to a method for determining the content of paeoniflorin in a modified motherwort ointment, which belongs to the technical field of medical product manufacturing. Background technique [0002] Modified Motherwort Ointment is based on Motherwort Ointment, adding Angelica, Radix Rehmanniae, Radix Paeoniae Rubra, Chuanxiong and other ingredients. It has the effect of nourishing blood and regulating menstruation. It is mainly used to treat irregular menstruation, irregular blood color, lack of menstrual fluid and postpartum stasis. Abdominal pain and other illnesses. Since the main ingredient of the flavored motherwort ointment is motherwort ointment, the content monitoring of the active ingredients of the traditional flavored motherwort ointment is mainly to determine the effective ingredient stachyamine in the motherwort; however, with the continuous deepening of medical research and the continuous improvement of quality control level It...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 张先文梁延波沈浩黄斌
Owner 上海海虹实业(集团)巢湖今辰药业有限公司
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