Swine interleukin-16 long-acting slowly-released microsphere preparation for injection and preparation method thereof
A slow-release microsphere preparation and interleukin technology, which is applied to non-active ingredient medical preparations, active ingredient-containing medical preparations, antiviral agents, etc., can solve the inconvenience of clinical application, short interval time, and short half-life and other issues, to achieve the effect of preventing and controlling epidemic diseases, especially viral diseases, convenient operation, and promoting growth
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] The long-acting sustained-release microsphere preparation for porcine interleukin-16 injection in this embodiment uses porcine interleukin-16 as the coating object, and lactide-glycolide copolymer with a molecular weight of 5000 Daltons as the microsphere. Spheroid carrier, prepared by:
[0033] 1) Add porcine interleukin-16 into the aqueous solution containing zinc salt, stir to make porcine interleukin-16 and Zn 2+ Fully combined, then add a gelatin solution with a concentration of 10mg / ml (the gelatin solution contains 10% mannitol and 10% trehalose by mass fraction), and stir at 5000rpm for 30s to obtain the concentration of porcine interleukin-16 Be the mixture A of 6mg / ml; The total mass fraction of stabilizing agent in the described mixture A is 0.1%;
[0034] 2) Add lactide-glycolide copolymer into dichloromethane and dissolve to obtain a mixture B with a concentration of lactide-glycolide copolymer of 130mg / ml, according to the volume ratio of mixture A: mixtu...
Embodiment 2
[0045] The long-acting sustained-release microsphere preparation for porcine interleukin-16 injection in this embodiment uses porcine interleukin-16 as the coating object, and lactide-glycolide copolymer with a molecular weight of 70,000 Daltons as the microsphere. Spheroid carrier, prepared by:
[0046] 1) Add porcine interleukin-16 into the aqueous solution containing zinc salt, stir to make porcine interleukin-16 and Zn 2+ Fully combined, then add a gelatin solution with a concentration of 10mg / ml (the gelatin solution contains 10% mannitol and 10% trehalose by mass fraction), and stir at 5000rpm for 30s to obtain porcine interleukin-16 Concentration is the mixture A of 8mg / ml; The total mass fraction of stabilizer in described mixture A is 10%;
[0047] 2) Add lactide-glycolide copolymer into dichloromethane and dissolve to obtain a mixture B with a concentration of lactide-glycolide copolymer of 150mg / ml, according to the volume ratio of mixture A: mixture B is 0.5 Add ...
Embodiment 3
[0058] The long-acting sustained-release microsphere preparation for porcine interleukin-16 injection in this embodiment uses porcine interleukin-16 as the coating object, and lactide-glycolide copolymer with a molecular weight of 40,000 Daltons as the microsphere Spheroid carrier, prepared by:
[0059] 1) Add porcine interleukin-16 into the aqueous solution containing zinc salt, stir to make porcine interleukin-16 and Zn 2+ Fully combined, then add a gelatin solution with a concentration of 10mg / ml (the gelatin solution contains 10% mannitol and 10% trehalose by mass fraction), and stir at 5000rpm for 30s to obtain the concentration of porcine interleukin-16 Be the mixture A of 7mg / ml; The total mass fraction of stabilizing agent in described mixture A is 5%;
[0060] 2) Add lactide-glycolide copolymer into dichloromethane and dissolve to obtain a mixture B with a concentration of lactide-glycolide copolymer of 140mg / ml, according to the volume ratio of mixture A: mixture B ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
| concentration | aaaaa | aaaaa |
| particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More