Biodegradable Polyurethane with Gradient Elastic Modulus and Its Fabricated Tissue Engineering Fibrous Scaffold

A technology of elastic modulus and fiber scaffold, which is applied in the field of biodegradable polyurethane with gradient elastic modulus and the tissue engineering fiber scaffold prepared by it, which can solve the differences in cell types, matrix composition and mechanical properties, and the mechanical properties of bionic fibrous annulus Can not achieve mechanical properties, complex microstructure and other problems, to achieve the effect of good biodegradability, excellent mechanical properties, good biocompatibility

Active Publication Date: 2016-09-21
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent research on tissue engineering of annulus fibrosus mainly tends to construct engineering scaffolds by simulating the oblique interlaced microstructure of actual annulus fibrosus tissue, which greatly improves the compressive modulus of bionic annulus fibrosus, but due to the collagen and The level of matrix such as glycosaminoglycan is far from that of the actual tissue, and the mechanical properties of the bionic fibrous annulus are far from those of the actual tissue.
The main reason is that most of the annulus fibrosus scaffolds currently studied are single-matrix materials, but the actual annulus fibrosus tissue is not only complex in microstructure, but also has obvious differences in cell types, matrix composition and mechanical properties in each region along the radial direction.

Method used

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  • Biodegradable Polyurethane with Gradient Elastic Modulus and Its Fabricated Tissue Engineering Fibrous Scaffold
  • Biodegradable Polyurethane with Gradient Elastic Modulus and Its Fabricated Tissue Engineering Fibrous Scaffold
  • Biodegradable Polyurethane with Gradient Elastic Modulus and Its Fabricated Tissue Engineering Fibrous Scaffold

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment one, elastic modulus is the synthesis of PECUU1 of 13.4MPa

[0038] Under the protection of argon, 10 g of dried PEO-PPO-PEO (1100 Da) was dissolved in toluene (15% by mass fraction), and then a toluene solution of 1,6-diisocyanate (3.05gHDI, mass fraction 15%), after adding 40 mg of the catalyst stannous octoate, react in an oil bath at 75° C. for 4 hours. Then, cool in an ice bath, add dropwise a DMF solution (1% by mass fraction) containing 1.60 g of butanediamine, and keep stirring to keep the solution clear. After reacting overnight, the precipitate was washed twice in a mixed solvent of ethanol / water (v / v, 30 / 70) and dried in vacuo. The FT-IR characterization shows that its structure is polyether polyurethane, and the mechanical tensile test is performed after casting into a film, and its elastic modulus is 13.4MPa.

Embodiment 2

[0039] Embodiment two, elastic modulus is the synthesis of PECUU2 of 6.4MPa

[0040] Under the protection of argon, dissolve 6.5g of dried PEO-PPO-PEO (1100Da) in 85mL of toluene, then add 9.65g of trimethylene carbonate monomer, add 0.48g of stannous octoate catalyst, and polymerize at 110°C React for 24 hours. After cooling down to room temperature, a toluene solution of 1,6-diisocyanate (1.98 g HDI, 15% by mass) was added under stirring conditions, and the temperature was raised to 75° C. for 4 hours. After cooling in an ice bath, a DMF solution (1% by mass fraction) containing 1.04 g of butanediamine was added dropwise, and kept stirring to keep the solution clear. After reacting overnight, the precipitate was washed twice in a mixed solvent of ethanol / water (v / v, 30 / 70) and dried in vacuo. The FT-IR characterization shows that its structure is polycarbonate polyurethane PECUU, and the mechanical tensile test is performed after casting into a film, and its elastic modulu...

Embodiment 3

[0041] Embodiment three, elastic modulus is the synthesis of PECUU3 of 5.1MPa

[0042] Under the protection of argon, dissolve 5.0 g of dried PEO-PPO-PEO (1100 Da) in 100 mL of toluene, then add 14.84 g of trimethylene carbonate monomer, add 0.37 g of stannous octoate catalyst, and polymerize at 110°C React for 24 hours. After cooling to room temperature, a toluene solution of 1,6-diisocyanate (1.53 g HDI, 15% by mass) was added under stirring conditions, and the temperature was raised to 75° C. for 4 hours. After cooling in an ice bath, a DMF solution containing 0.8 g of butanediamine (1% by mass fraction) was added dropwise, and kept stirring to keep the solution clear. After reacting overnight, the precipitate was washed twice in a mixed solvent of ethanol / water (v / v, 30 / 70) and dried in vacuo. The FT-IR characterization shows that its structure is polycarbonate polyurethane PECUU, and the mechanical tensile test is performed after casting into a film, and its elastic mod...

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PUM

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Abstract

The invention discloses a biodegradable polyurethane and its preparation and application in fiber annulus tissue engineering. The biodegradable polyurethane has a gradient elastic modulus of 1.5-15.0 MPa and can prepare tissue engineering fiber scaffolds by electrospinning , stem cells derived from annulus fibrosus can proliferate on the scaffold, and the differentiation of stem cells is regulated by the elastic modulus. The expression of collagen type I gene is relatively high on the scaffold with high elastic modulus; while on the fibrous scaffold with lower elastic modulus , the gene expression of collagen type II gene and glycosaminoglycan was relatively high. The results of cell traction test showed that the cell traction force on the fiber scaffold with higher elastic modulus was relatively small, and the cell traction force on the fiber scaffold with lower elastic modulus was relatively large, which was consistent with the difference in the radial area of ​​the actual fibrous annulus. This provides the possibility to develop annulus fibrosus tissue engineering scaffolds that can mimic regional differences in annulus fibrosus.

Description

technical field [0001] The present invention relates to a biodegradable polyurethane with adjustable elastic modulus and its preparation method, and its application in fibrous annulus tissue engineering; in particular, it relates to the synthesis of biodegradable polyurethane with gradient elastic modulus and the polymer in Application of bionic annulus fibrosus regional mechanical difference structure in annulus fibrosus tissue engineering. Background technique [0002] Intervertebral disc degeneration is one of the main clinical causes of low back pain. Current surgical treatment or biological intervention can only relieve clinical symptoms, but cannot fundamentally prevent intervertebral disc degeneration. The emergence of tissue engineering in recent years provides a new strategy for permanent repair of intervertebral discs, and annulus fibrosus tissue engineering is one of the key links in the successful construction of tissue-engineered intervertebral discs. Recent re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G18/44A61L27/18A61L27/38A61L27/58
Inventor 李斌朱彩虹杨惠林
Owner SUZHOU UNIV
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