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Method for extracting carthamin yellow from safflower carthamus

A technology of safflower yellow pigment and safflower, which is applied in the direction of chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of high equipment requirements, loss of flavonoid components, low extraction rate, etc., to increase the diffusion area, The reaction conditions are mild and do not destroy the effect of the ingredients

Inactive Publication Date: 2014-12-24
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solvothermal extraction consumes a lot of energy and requires a higher temperature, which is easy to cause the loss of flavonoids and low extraction rate; while supercritical CO 2 Fluid extraction method, such as patent CN101019909B (a method for supercritical extraction of safflower), that is, after pretreatment of safflower, directly use CO 2 Or low-molecular-weight hydrocarbon supercritical liquid extracts raw materials to obtain non-polar safflower volatile oil and polar safflower total flavonoids, etc. This method requires high equipment and is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Put the impurity-removed safflower into a container, add 5 times of 55% ethanol solution, place it on a traditional Chinese medicine extractor to crush and extract the safflower for 10 minutes; take it out, adjust the pH value of the extract to 5.5, and add 3‰ of the raw material quality put cellulase into a water bath, enzymolysis temperature is 60°C, time is 2.0h; raise the temperature of water bath to 80°C, high temperature inactivation 10min; adjust the pH value of the solution to neutral, filter and concentrate to 1 / 5 of the original volume; the concentrate was purified by pretreated D-101 macroporous adsorption resin, eluted with 5BV of 40% ethanol, and the eluate was collected; concentrated under reduced pressure and spray-dried to obtain the safflower yellow product, The extraction rate reaches 79%.

Embodiment 2

[0020] Put the impurity-removed safflower into a container, add 10 times of 45% ethanol solution, place it on a traditional Chinese medicine extractor to crush and extract the safflower for 10 minutes; take it out, adjust the pH value of the extract to 4.5, and add 4‰ of the raw material quality put cellulase into a water bath, enzymolysis temperature is 50°C, time is 3.0h; raise the temperature of water bath to 80°C, high temperature inactivation 10min; adjust the pH value of the solution to neutral, filter and concentrate to 1 / 10 of the original volume; the concentrate was purified by pretreated AB-8 macroporous adsorption resin, eluted with 4BV of 30% ethanol, and the eluate was collected; concentrated under reduced pressure and spray-dried to obtain the safflower yellow product, The extraction rate reaches 85%.

Embodiment 3

[0022] Put the impurity-removed safflower into a container, add 8 times of 50% ethanol solution, place it on a traditional Chinese medicine extractor to crush and extract the safflower for 10 minutes; take it out, adjust the pH value of the extract to 5.0, and add 5‰ of the raw material quality put cellulase into a water bath, enzymolysis temperature is 50°C, and time is 2.5h; raise the temperature of the water bath to 80°C, and inactivate at high temperature for 10 minutes; adjust the pH of the solution to be neutral, filter, and concentrate to 1 / 8 of the original volume; the concentrate was purified by pretreated HPD-200 macroporous adsorption resin, eluted with 3BV of 20% ethanol, and the eluate was collected; concentrated under reduced pressure and spray-dried to obtain the safflower yellow product, The extraction rate reached 82%.

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Abstract

The invention relates to a method for extracting carthamin yellow from safflower carthamus and in particular relates to a method combining a flash extraction technology with an enzymolysis process. The method comprises the following steps: a, putting safflower carthamus subjected to impurity removal into a container, adding an ethanol solution, and crushing the safflower carthamus on a traditional Chinese medicine extractor for extraction; b, adjusting the pH value of extraction liquid to be 4.5-5.5, adding cellulose which accounts for 0.3wt%-0.5wt% of the raw material, and performing enzymolysis at 40-60 DEG C for 2.0-3.0 hours; c, inactivating at the high temperature; d, adjusting the pH value of the solution to be 7, filtering, and performing reduced pressure concentration; e, purifying concentrated liquid by adopting macroporous adsorption resin, eluting by an ethanol solution, and collecting eluate; and f, performing reduced pressure concentration and spray-drying to obtain carthamin yellow. According to the method, the safflower carthamus is adopted as the raw material, the flash extraction and enzymolysis combined process is fully utilized, carthamin yellow in the safflower carthamus is dissolved out to the maximum extent, the extraction time is greatly shortened, the cost is relatively low, the environment is well protected, and the industrial production is facilitated.

Description

technical field [0001] The invention relates to a method for extracting safflower yellow from safflower, in particular to a method for extracting safflower yellow by applying flash extraction technology combined with enzymatic hydrolysis. Background technique [0002] Safflower is the dried flower of Safflower safflower, which is one of the main traditional Chinese medicines for promoting blood circulation and removing blood stasis. There are currently 13 species of safflower in the world, of which there is only one in my country. The chemical components of safflower are mainly flavonoids and fatty oils, among which the chalcone compound safflower yellow is the main active ingredient of safflower. Pharmacological studies have shown that safflower yellow has 255 pharmacological effects such as expanding coronary artery, anti-oxidation, protecting myocardium, lowering blood pressure, immunosuppression and brain protection. In addition, safflower yellow is a natural pigment w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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