Tobacco-derived cellulase-producing microbe strain and application thereof

A technology of cellulase and microorganisms, applied in the direction of microorganism-based methods, microorganisms, applications, etc., can solve the problems of high cost and application that have not yet been publicly reported, and achieve the goal of improving internal quality, good adaptability, and reducing cellulose content Effect

Inactive Publication Date: 2014-12-24
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese Patent Document No. CN101912149A discloses a method for improving the quality of tobacco stems by combining steam explosion and enzyme treatment technology. In this invention, the moistened tobacco stems are steam-exploded first, and then ligninase and cellulase are added thereto. , to reduce the impact of cellulose on the quality of tobacco stems, but this method is costly and has certain requirements for equipment
At present, there is no public report on the application of this microbial strain in tobacco

Method used

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  • Tobacco-derived cellulase-producing microbe strain and application thereof
  • Tobacco-derived cellulase-producing microbe strain and application thereof
  • Tobacco-derived cellulase-producing microbe strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0035] Add Trichoderma harzianum strain TC10-13 into sterile water to make spore suspension (spore concentration is 10 6 individual / mL),

[0036]Inoculate 7% into a 250 mL Erlenmeyer flask containing 50 mL of enzyme-producing medium for fermentation. The culture temperature was 28°C, the shaker speed was 200 rpm, and the culture time was 96 h. The cellulase activity of the obtained crude enzyme solution was determined to be 37.83 U / mL.

[0037] The composition of enzyme production medium is (g / L): wheat straw powder 15.0, yeast extract 4.0, MgSO 4 7H 2 O 0.3 g, Tween-80 2.0 mL, initial pH 5.0.

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Abstract

The invention relates to a tobacco-derived cellulase-producing microbe strain and application thereof in tobacco. The invention is characterized in that the microbe strain is a Trichoderma harzianum TC10-13 strain which is collected by China General Microbiological Culture Collection Center on December 17th, 2013, and the collection number is CGMCC No.8587. The ITS sequence of the strain is the main characteristic reference for identifying the strain. The application method comprises the following steps: by using wheat straw powder or cabo powder as a carbon source and a yeast extract as a nitrogen source, carrying out liquid fermentation to obtain a crude enzyme solution, and proportionally spraying the fermentation crude enzyme solution onto the tobacco shred, sheet tobacco and cabo to obviously lower the cellulose content in tobacco. The cellulose degradation strain is convenient to apply and can enhance the inherent quality of the tobacco.

Description

[0001] technical field [0002] The invention relates to a cellulase-producing microbial strain derived from tobacco and its application in tobacco, belonging to the field of microbial fermentation and its application. Background technique [0003] Cellulose is a polysaccharide with the molecular formula (C 6 h 10 o 5 )n. Cell wall substances in tobacco are composed of cellulose, hemicellulose, lignin and other substances. These substances play a role in maintaining the structure of tobacco cell walls, accounting for about 26% to 35% of the dry matter weight of tobacco, of which cellulose accounts for about 10% of the dry matter. 13%~18% of the total weight (Yan Keyu, Yan Hongyang, Li Xingbo, et al. 2005. Comparative analysis of cell wall substances in flue-cured tobacco leaves. Tobacco Science and Technology, 2005 (10): 6-11). [0004] Cellulose can promote the combustibility of tobacco leaves to a certain extent, and enhance the fire holding power of tobacco leaves. H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42A24B15/20C12R1/885
Inventor 郭建华宋纪真奚家勤孟庆华王信民周汉平
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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