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Preparation of immunizing antigens and envelope antigens for detecting furazolidone metabolites

The technology of furazolidone and immunization antigen is applied in the field of preparation of immunization antigen and coated antigen, which can solve the problems of residual metabolites and the like, and achieve the effects of improving immunogenicity, improving specificity and improving coupling ratio.

Inactive Publication Date: 2014-12-24
佛山市质量计量监督检测中心
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Problems solved by technology

However, studies have shown that the half-life of the original drug is usually only a few hours, while its metabolites can still remain in animal tissues for several weeks

Method used

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  • Preparation of immunizing antigens and envelope antigens for detecting furazolidone metabolites
  • Preparation of immunizing antigens and envelope antigens for detecting furazolidone metabolites
  • Preparation of immunizing antigens and envelope antigens for detecting furazolidone metabolites

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Embodiment Construction

[0020] 1 Synthesis of furazolidone metabolite hapten by acetal method

[0021] 200 mL of ethanol was added into a 500 mL round bottom flask, furazolidone (10.2 g, 0.1 mol) and 6-oxohexanoic acid (15.6 g, 0.12 mol) were added in sequence, and heated to reflux for 3 hours. Heating was stopped, and the reaction solution was naturally cooled to room temperature, and left to stand overnight. After suction filtration, the filter cake was washed with ethanol (20.0 mL×3), and dried at 50° C. for 5 hours to obtain 20.4 g of carboxyl-containing furazolidone derivatives. Preparation of furazolidone metabolite hapten (I).

[0022] 2 Synthesis of Furazolidone Metabolite Immune Antigen by Active Thioester Method

[0023] Dissolve the furazolidone hapten (0.214g, 1.0mmol) in 30mL of dimethylformamide (Nimethylformamide, DMF), cool to 0-4°C, add 3-phosphorylated diphenyl ester benzo[D]oxane under stirring Azol-2-one [2(3H)-Benzoxazolone,3-(diphenoxyphosphinyl)-(9CI), DPBO] (0.351g, 1.0mmol...

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Abstract

The invention discloses a preparation method of immunizing antigens and envelope antigens which are used for detecting furazolidone metabolites. The preparation method is characterized by comprising the step of coupling furazolidone metabolite hapten with carrier proteins to prepare the immunizing antigens and the envelope antigens for the furazolidone metabolites by an active sulphur ester method, wherein the furazolidone metabolite hapten is prepared by introducing spacer arms into the furazolidone metabolites by an acetal method. According to the preparation method, with an optimization coupling technical line, structural optimization of the immunizing antigens and the envelope antigens is realized to improve the quality of an antibody, and therefore, the sensitivity and the specificity for quickly detecting the furazolidone metabolites by virtue of immunity colloid gold are further improved.

Description

technical field [0001] The invention relates to the technical field of detection of furazolidone metabolites in animal-derived foods, in particular to the preparation of immune antigens and coating antigens for detecting furazolidone metabolites. Background technique [0002] Furazolidone, namely 3-(5-nitrofurfural amino)-2-oxazolidinone, also known as furazolidone, is the most representative kind of broad-spectrum nitrofuran antibiotics. The basic structure of this type of drug is a furan ring, with a nitro group attached to the fifth carbon atom, which has a strong antibacterial effect. Furazolidone is cheap, has definite curative effect, and is widely used in livestock and poultry and aquaculture. Furazolidone metabolizes rapidly in the living body and is completely metabolized within a short period of time. Studies have shown that furazolidone can be metabolized into 3-amino-2-oxazolidinone (AOZ) in animals. Some of the metabolized compound molecules combine with histo...

Claims

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Application Information

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IPC IPC(8): G01N33/531
CPCG01N33/532
Inventor 潘嘉慧田峻沈国权赵亮亮杨辉朱海云
Owner 佛山市质量计量监督检测中心
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