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Haemophilus parasuis disease vaccine composition, preparation method and application thereof

A technology for Haemophilus suis disease and vaccine composition, which is applied in the directions of drug combination, antibacterial drugs, respiratory system diseases, etc., can solve the problems that vaccines are difficult to achieve immune effect, interference, etc., and achieve effective treatment and prevention of haemophilus parasuis Bacillus related diseases, unsatisfactory solution effect, good immune effect

Inactive Publication Date: 2014-12-31
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pathogenic strains of Haemophilus parasuis and multiple serotype infections have made it difficult for existing vaccines to achieve the expected immune effect
Due to the lack of suitable virus strain preparation and the interference between various antigens, it is an urgent problem for the breeding industry to provide a vaccine that can effectively prevent and treat Haemophilus parasuis disease caused by the new pathogen of Haemophilus parasuis

Method used

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  • Haemophilus parasuis disease vaccine composition, preparation method and application thereof
  • Haemophilus parasuis disease vaccine composition, preparation method and application thereof
  • Haemophilus parasuis disease vaccine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation and efficacy test of embodiment 1 Haemophilus parasuis inactivated vaccine

[0039] 1. Strains

[0040] a. Haemophilus parasuis serotype 4 JS strain, taxonomic name: Haemophilus parasuis JS (Haemophilus parasuis JS), isolated and identified by Pulaike Bioengineering Co., Ltd., has been carried out in the China Center for Typical Cultures Deposit, date of deposit: May 18, 2011, deposit number: CCTCC M2011172.

[0041] b. Haemophilus parasuis serotype 5 ZJ strain, classified and named: Haemophilus parasuis ZJ (Haemophilus parasuis ZJ), isolated and identified by Pulaike Bioengineering Co., Ltd., and has been carried out in the China Center for Typical Cultures Deposit, date of deposit: May 18, 2011, deposit number: CCTCC M2011173.

[0042] c. HeB strain of Haemophilus parasuis serotype 12, classified and named: Haemophilus parasuis HeB (Haemophilus parasuis HeB), which was isolated and identified by Pulaike Bioengineering Co., Ltd., and has been carried out ...

Embodiment 2

[0079] Embodiment 2 cross-protection test

[0080] Experiments were carried out in the experimental animal room of Pulaike Bioengineering Co., Ltd. Take the vaccine 2, vaccine 5 and vaccine 8 prepared above, and each group of vaccines uses 15 28-day-old healthy susceptible pigs (raised and provided by the experimental animal room of Pulaike Bioengineering Co., Ltd.), and injects 2 mL each intramuscularly. Containing one dose, after 28 days, the immune test pigs injected with each group of vaccines were randomly divided into three groups, and challenged with the serotype 4, 5 and 12 strains of Haemophilus parasuis respectively. At the same time, 15 control pigs were selected, divided into three groups, and challenged with the H. parasuis serotype 4, 5, and 12 strains respectively. details as follows:

[0081] Select 15 pigs for the immunization test of Haemophilus parasuis vaccine 2 prepared in Example 1, divide them into three groups at random, and inject 1 morbidity dose of...

Embodiment 3

[0089] The novel inactivated vaccine of Haemophilus parasuis disease prepared by different adjuvants and efficacy test of embodiment 3

[0090] Utilize different adjuvants to prepare Haemophilus parasuis inactivated vaccines: prepared from the Haemophilus parasuis serotype 4 strains prepared in Example 1 that have been removed from the capsule, inactivated, concentrated and passed the safety inspection, ready to use Antigens were prepared from Haemophilus parasuis serotype 4 strains and mixed with Montanide IMS1313VG, Montanide IMS251C VG, Montanide ISA15A VG, Montanide GEL PR or aluminum hydroxide gel adjuvants to prepare vaccines. The specific formula is shown in Table 4.

[0091] Preparation of aqueous adjuvant vaccine: inactivated Haemophilus parasuis vaccine made with Montanide IMS1313VG or Montanide IMS251C VG or Montanide GEL PR as adjuvant. The specific method is as follows: Concentrate the bacterial solution with a hollow fiber ultrafilter, discard the supernatant, a...

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Abstract

The invention provides a Haemophilus parasuis disease vaccine composition, which contains an immune dose of capsule removed Haemophilus parasuis whole bacterium antigen and a veterinarily acceptable adjuvant. The capsule removed Haemophilus parasuis disease vaccine composition provided by the invention not only provides good immunoprotection to haemophilus parasuis of one same serotype, but also can have good preventive and therapeutic effects on Haemophilus parasuis diseases caused by currently epidemic Haemophilus parasuis of a variety of serotypes in China.

Description

technical field [0001] The invention relates to a Haemophilus parasuis vaccine, its preparation method and application. Background technique [0002] Haemophilus parasuis, also known as fibrinous serositis multiplex and arthritis, is a disease characterized clinically by elevated body temperature, joint swelling, dyspnea, polyserositis, arthritis, and high infectious disease. The disease, also known as Glsser's Disease, was once thought to be a sporadic disease caused by stress, but was later confirmed to be caused by Haemophilus parasuis (Hps). [0003] Haemophilus parasuis can affect young pigs from 2 weeks to 4 months, mainly after weaning and nursery stage, usually seen in pigs of 5 to 8 weeks, the incidence is generally 10% to 15%, severe The mortality rate can reach 50%. The main clinical symptoms were cough, dyspnea, emaciation, lameness, and rough coat; the main necropsy lesions were fibrinous pleurisy, pericarditis, peritonitis, arthritis, and meningitis. In add...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61P31/04A61P11/14A61P11/00
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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