Test paper card for detecting fumonisin B1 and application thereof
A technology of fumonisins and test paper cards, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable grass-roots promotion and use, high degree of instrumentation, and slow analysis speed, and achieve easy promotion and use, high sensitivity, and storage simple effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Example 1 Preparation of fumonisin B1 detection test paper card
[0041] The preparation method of the test paper card mainly comprises the following steps:
[0042] 1) Prepare a conjugate release pad sprayed with fumonisin B1 monoclonal antibody-colloidal gold marker;
[0043] 2) Prepare a reaction membrane with a detection line coated with a fumonisin B1 hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody;
[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip card.
[0045] The following step-by-step detailed description:
[0046] 1. Preparation of fumonisin B1 hapten
[0047] Add the mixture of 36mg fumonisin and 1ml dimethylsulfoxide (DMSO) dropwise slowly at room temperature to 1ml DMSO solution of 14mg terephthalaldehyde. After the dropwise addition, react at room temperature to 60°...
Embodiment 2
[0079] Example 2 Detection of Fumonisin B1 Residues in Samples
[0080] 1. Pretreatment of samples
[0081] Weigh 4.0g±0.05g crushed sample into a 50ml polystyrene centrifuge tube, add 3g NaCl, 5ml methanol and 5ml deionized water, vortex for 3min with a vortexer; centrifuge at room temperature (20-25℃) over 3000g for 5min ; Pipette 100 μl supernatant and 200 μl sample reconstitution solution (0.02mol / L phosphate buffer) and mix well before testing.
[0082] 2. Test with test paper card
[0083] Use a straw to draw the sample solution to be tested and drop 2-3 drops vertically into the sample hole, start timing when the liquid flows, react for 7-10 minutes, and judge the result. Other times are invalid.
[0084] 3. Analyze test results
[0085] Negative (-): C-line color development, T-line color development, no matter the color depth, it means that the concentration of fumonisin B1 in the sample is lower than the detection limit, such as Figure 4 a.
[0086] Positive (+...
Embodiment 3
[0088] Example 3 Example of sample detection
[0089] 1. Detection limit test
[0090] Take blank grains (soybean, corn) and feed, add fumonisin B1 to the final concentration of 10, 20, and 40 μg / kg, respectively, take a test card for detection, and repeat the measurement three times for each sample.
[0091] When testing soybeans, corn, and feed samples with test paper cards, when the added concentration of fumonisin B1 is 10 μg / kg, two red lines visible to the naked eye are displayed on the test paper card, which is negative; when the added concentration of fumonisin B1 is When it is 20 and 40 μg / kg, the quality control line of the test paper card develops color, but the detection line does not develop color and is positive, indicating that the detection limit of this test paper card for fumonisin B1 in grain and feed samples is 20 μg / kg.
[0092] 2. False positive rate and false negative rate test
[0093] Take 20 positive samples of soybeans, corn, and feed, and 20 negat...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More