Screening method for glyphosate-resisting medicago sativa plants

An alfalfa and glyphosate-resistant technology, applied in botany equipment and methods, plant genetic improvement, soilless cultivation, etc., can solve the problems of screening methods for herbicide-resistant alfalfa plants that have not been disclosed or published, and achieve effective results quick effect

Inactive Publication Date: 2015-01-07
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Yet, before the application of the present invention, the screening method of herbicide-resistant alfalfa plants has not been disclosed or published in China

Method used

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  • Screening method for glyphosate-resisting medicago sativa plants
  • Screening method for glyphosate-resisting medicago sativa plants
  • Screening method for glyphosate-resisting medicago sativa plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: a kind of medium screening method of glyphosate-resistant alfalfa plant is characterized in that comprising the steps:

[0047] (1) MS medium to germinate seeds

[0048] Select alfalfa seeds; prepare MS culture plates for later use; carry out seed disinfection under sterile conditions: soak seeds in 100% alcohol for 0.5 min; soak in 2% sodium hypochlorite solution for 15 min; ddH 2 O Wash the seeds 10 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 22°C, 18 hours of light, 6 hours of darkness to germinate; the radicle breaks through the testa For germination, the 6th day, the average germination rate reaches more than 50%, stand-by;

[0049] (2) Dose screening

[0050] There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Concentration of glyp...

Embodiment 2

[0059] Embodiment 2: a kind of culture medium screening method of glyphosate-resistant alfalfa plant, is characterized in that comprising the steps:

[0060] (1) MS medium to germinate seeds

[0061] Select alfalfa seeds; prepare MS medium culture plates for later use; carry out seed disinfection under sterile conditions: soak seeds in 75% alcohol for 1 min; soak in 2% sodium hypochlorite solution for 10 min; ddH 2 O Wash the seeds 5 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 20°C, 16 hours of light, 8 hours of darkness to germinate; the radicle breaks through the seed coat For germination, the 5th day, the average germination rate reaches more than 50%, stand-by;

[0062] (2) Dose screening

[0063] There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Conce...

Embodiment 3

[0072] Embodiment 3: a kind of medium screening method of glyphosate-resistant alfalfa plant is characterized in that comprising the steps:

[0073] (1) MS medium to germinate seeds

[0074] Select alfalfa seeds; prepare MS medium culture plates for use; carry out seed disinfection under sterile conditions: soak seeds in 60% alcohol for 2 minutes; soak in 2% sodium hypochlorite solution for 5 minutes; ddH 2 O Wash the seeds 3 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 18°C, 0h light, 24h dark to germinate; the radicle breaks through the testa For germination, the 3rd day, the average germination rate reaches more than 50%, stand-by;

[0075] (2) Dose screening

[0076]There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Concentration of glyphosate medium, ea...

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Abstract

The invention mainly provides a medicago sativa plant resistant to herbicide and a method, particularly relates to a method for screening medicago sativa plants resistant to the herbicide by utilizing chemical mutagen EMS (ethyl methane sulfonate) for mutagenesis of medicago sativa, and discloses a culture medium screening method for glyphosate-resisting medicago sativa plants. The method is characterized by including the following steps: (1) germinating seeds by an MS culture medium; (2) performing dosage screening; (3) subjecting medicago sativa seeds to mutagenesis by median lethal dose of the EMS; (4) screening the glyphosate-resisting medicago sativa plants; (5) rescreening the resistant plants. The method has the advantages of being simple and easy to implement, taking effect fast and capable of allowing single plants of herbicide-resistant medicago sativa to be obtained within a short time. A mutagenesis way is adopted. The chemical mutagen EMS is utilized to mutate the medicago sativa seeds to generate point mutation, the single plants of the herbicide-resistant medicago sativa are screened by two ways of the culture medium and soil screening, new germplasm resources are provided for conventional breeding, and certain references are provided for herbicide-resistant breeding of other plants.

Description

technical field [0001] The present invention generally provides alfalfa plants and methods for producing herbicide-resistant alfalfa plants. Specifically, it involves using a chemical mutagen EMS to mutate alfalfa and screen out alfalfa plants resistant to herbicides. Background technique [0002] Alfalfa (Medicago sativa L.), as a high-quality leguminous forage, has been dubbed the "King of Grass" and "Queen of Feed". It is one of the most important cultivated forages and a high-quality legume with good prospects for industrialization. , has the characteristics of high yield, high feed value (crude protein content 21%), good palatability, and wide application. It plays a huge role in my country's agricultural and animal husbandry production and ecological economic construction. There are many kinds of weeds in alfalfa field, both dicotyledonous weeds and monocotyledonous weeds, among which the damage of dicotyledonous weeds is the most serious. Weeds compete with alfalfa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01H1/04A01H1/06
CPCA01H1/04A01H1/06
Inventor 张吉宇吴凡段珍狄红艳霍雅馨张代玉
Owner LANZHOU UNIVERSITY
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