Screening method for glyphosate-resisting medicago sativa plants
An alfalfa and glyphosate-resistant technology, applied in botany equipment and methods, plant genetic improvement, soilless cultivation, etc., can solve the problems of screening methods for herbicide-resistant alfalfa plants that have not been disclosed or published, and achieve effective results quick effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0046] Embodiment 1: a kind of medium screening method of glyphosate-resistant alfalfa plant is characterized in that comprising the steps:
[0047] (1) MS medium to germinate seeds
[0048] Select alfalfa seeds; prepare MS culture plates for later use; carry out seed disinfection under sterile conditions: soak seeds in 100% alcohol for 0.5 min; soak in 2% sodium hypochlorite solution for 15 min; ddH 2 O Wash the seeds 10 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 22°C, 18 hours of light, 6 hours of darkness to germinate; the radicle breaks through the testa For germination, the 6th day, the average germination rate reaches more than 50%, stand-by;
[0049] (2) Dose screening
[0050] There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Concentration of glyp...
Embodiment 2
[0059] Embodiment 2: a kind of culture medium screening method of glyphosate-resistant alfalfa plant, is characterized in that comprising the steps:
[0060] (1) MS medium to germinate seeds
[0061] Select alfalfa seeds; prepare MS medium culture plates for later use; carry out seed disinfection under sterile conditions: soak seeds in 75% alcohol for 1 min; soak in 2% sodium hypochlorite solution for 10 min; ddH 2 O Wash the seeds 5 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 20°C, 16 hours of light, 8 hours of darkness to germinate; the radicle breaks through the seed coat For germination, the 5th day, the average germination rate reaches more than 50%, stand-by;
[0062] (2) Dose screening
[0063] There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Conce...
Embodiment 3
[0072] Embodiment 3: a kind of medium screening method of glyphosate-resistant alfalfa plant is characterized in that comprising the steps:
[0073] (1) MS medium to germinate seeds
[0074] Select alfalfa seeds; prepare MS medium culture plates for use; carry out seed disinfection under sterile conditions: soak seeds in 60% alcohol for 2 minutes; soak in 2% sodium hypochlorite solution for 5 minutes; ddH 2 O Wash the seeds 3 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 18°C, 0h light, 24h dark to germinate; the radicle breaks through the testa For germination, the 3rd day, the average germination rate reaches more than 50%, stand-by;
[0075] (2) Dose screening
[0076]There are 9 treatments for glyphosate, the concentrations are 0mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L, 500mg / L, 600mg / L, 800mg / L, the configurations are different Concentration of glyphosate medium, ea...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com