Screening method for glyphosate-resisting medicago sativa plants

An alfalfa and glyphosate-resistant technology, applied in botany equipment and methods, plant genetic improvement, soilless cultivation, etc., can solve the problems of screening methods for herbicide-resistant alfalfa plants that have not been disclosed or published, and achieve effective results quick effect
CN104255403AInactive Publication Date: 2015-01-07LANZHOU UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LANZHOU UNIVERSITY
Publication Date
2015-01-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention mainly provides a medicago sativa plant resistant to herbicide and a method, particularly relates to a method for screening medicago sativa plants resistant to the herbicide by utilizing chemical mutagen EMS (ethyl methane sulfonate) for mutagenesis of medicago sativa, and discloses a culture medium screening method for glyphosate-resisting medicago sativa plants. The method is characterized by including the following steps: (1) germinating seeds by an MS culture medium; (2) performing dosage screening; (3) subjecting medicago sativa seeds to mutagenesis by median lethal dose of the EMS; (4) screening the glyphosate-resisting medicago sativa plants; (5) rescreening the resistant plants. The method has the advantages of being simple and easy to implement, taking effect fast and capable of allowing single plants of herbicide-resistant medicago sativa to be obtained within a short time. A mutagenesis way is adopted. The chemical mutagen EMS is utilized to mutate the medicago sativa seeds to generate point mutation, the single plants of the herbicide-resistant medicago sativa are screened by two ways of the culture medium and soil screening, new germplasm resources are provided for conventional breeding, and certain references are provided for herbicide-resistant breeding of other plants.
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Description

technical field

[0001] The present invention generally provides alfalfa plants and methods for producing herbicide-resistant alfalfa plants. Specifically, it involves using a chemical mutagen EMS to mutate alfalfa and screen out alfalfa plants resistant to herbicides. Background technique

[0002] Alfalfa (Medicago sativa L.), as a high-quality leguminous forage, has been dubbed the "King of Grass" and "Queen of Feed". It is one of the most important cultivated forages and a high-quality legume with good prospects for industrialization. , has the characteristics of high yield, high feed value (crude protein content 21%), good palatability, and wide application. It plays a huge role in my country's agricultural and animal husbandry production and ecological economic construction. There are many kinds of weeds in alfalfa field, both dicotyledonous weeds and monocotyledonous weeds, among which the damage of dicotyledonous weeds is the most serious. Weeds compete with alfalfa...

Claims

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