Method for extracting apple polyphenol by ethyl alcohol solvent with assistance of pressure ultrasound

An apple polyphenol and ultrasonic-assisted technology, which is applied in the field of food processing, can solve the problems of increasing the yield of apple polyphenols and reducing the yield of polyphenols, and achieves the effects of low extraction cost, increased yield and simple equipment.

Inactive Publication Date: 2015-01-07
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the main problem in the existing apple polyphenol extraction technology is that "polyphenol oxidase can oxidize polyphenols and reduce the yield of polyphenols". In view of the fact that pressure can effectively enhance the cavitation effect of ultrasonic waves, the combination of pressure and ultrasonic waves can Effectively reducing enzyme activity and other factors, combining pressure and ultrasound to assist solvent extraction can effectively increase the yield of apple polyphenols

Method used

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  • Method for extracting apple polyphenol by ethyl alcohol solvent with assistance of pressure ultrasound
  • Method for extracting apple polyphenol by ethyl alcohol solvent with assistance of pressure ultrasound
  • Method for extracting apple polyphenol by ethyl alcohol solvent with assistance of pressure ultrasound

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Embodiment 1

[0024] The determination of apple polyphenol content in the present invention adopts Folin's phenol method (FC method). Take 2mL of apple polyphenol extract, add 1ml of folinol, mix well and let stand for 3 to 4 minutes, add 10% Na 2 CO 3 1ml, in a water bath at 25°C for 2 hours in the dark, and use a spectrophotometer to measure the absorbance at 765nm, using distilled water as a blank control. According to the standard curve equation of gallic acid, the yield of apple polyphenols was calculated.

Embodiment 2

[0026] The method for measuring the activity of apple polyphenol oxidase in the present invention is as follows: in the reaction system that 2ml citric acid buffer solution (pH=3.00) and 0.5ml 0.1mol / L catechol solution are formed, add apple polyphenol extract 2mL was mixed quickly, and the reagent was used as a blank, and the heat-inactivated (heated at 100°C for 1min) crude enzyme solution was used as a control, and the absorbance value (OD value) was measured at a wavelength of 420nm. Within 1 min, the change of OD with time was recorded every 15 s, and the enzyme activity was represented by the slope of the initial straight line segment (△OD / t). One enzyme activity unit (U) is defined as: the amount of enzyme required to cause a change in absorbance of 0.001 per minute under the assay conditions.

[0027] The apple pomace in the present invention is the fresh apple pomace obtained after the apples are squeezed by a juicer, and the moisture content is measured by an atmosph...

Embodiment 3

[0029] Put the red Fuji apple pomace in a pressure ultrasonic extraction kettle, add a 60% ethanol solution with a solid-liquid ratio of 1:25, adjust the pH value between 3.0 and 4.5, increase the pressure to 0.4MPa, and perform ultrasonic extraction. Ultrasonic frequency: 20KHz, ultrasonic power 200W, total ultrasonic treatment time 18min, working temperature: 70°C.

[0030] Controlled test: above-mentioned processing condition is directly treated with ultrasonic treatment without pressurization, carries out controlled test, measures the relative activity of polyphenol extraction rate and enzyme.

[0031] It was determined that the extraction rate of apple polyphenols obtained without pressure ultrasonic treatment was 0.374g / kg, and the extraction rate of apple polyphenols obtained through pressure ultrasonic extraction was 0.404g / kg. It can be seen that compared with the direct ultrasonic extraction method, the extraction rate of apple polyphenols under pressure ultrasonic t...

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Abstract

The invention discloses a method for extracting apple polyphenol by ethyl alcohol solvent with the assistance of pressure ultrasound. The method comprises the following steps: (a) selecting crushed and juiced apple residues as raw materials; (b) putting the apple residue into a pressure ultrasound device, adding 40%-80% ethyl alcohol solution in the pressure ultrasound device, wherein the weight ratio of the apple residue to the ethyl alcohol solution is 1 to (15-30), and the pH value is adjusted to be between 3.0 and 4.5; carrying out pressurization ultrasound treatment, wherein the treatment conditions are that the pressure range is 0.20-0.50MPa, the ultrasound frequency is 20KHz, the ultrasound power is 150W-250W, the total ultrasound treatment time is 9-20 minutes, and the working temperature is 55 DEG C-85 DEG C. After the selected ultrasound time is reached, a feed liquid is taken out, and is centrifuged, and then the solvent is recycled, and dried, thereby obtaining a crude apple polyphenol product.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a method for extracting apple polyphenols with the aid of pressure ultrasound and ethanol solvent. Background technique [0002] Apple polyphenols are the general term for phenolic compounds contained in apples, mainly including phenolic acids and flavonoids, among which cinnamic acid derivatives and flavanols account for about 90% of apple polyphenols. Studies have shown that apple polyphenols have various health effects such as anti-oxidation, antibacterial, scavenging free radicals, anti-tumor, suppression of hypertension, and prevention and treatment of cardiovascular and cerebrovascular diseases. They are a class of natural compounds with important physiological activities. At present, in the extraction of apple polyphenols, there are traditional simple solvent extraction methods, methods that combine solvent extraction with ultrasonic, microwave, and supercritical fluid extra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/30A61K8/97A61Q19/00A23L33/105
CPCA23V2002/00A61K8/347A61K8/97A61K2800/82A61Q19/08A23V2200/30A23V2250/21
Inventor 唐琳辛明亮苏树鹏张文龙
Owner SHANDONG NORMAL UNIV
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