Method for preparing obakulactone from waste liquid of extracting berberine from phellodendron bark

Abstract

The invention discloses a method for preparing obakulactone from an ethanol waste liquid of separating berberine from phellodendron bark, which comprises the following steps: by using a filtrate which is obtained by separating a crude product berberine from a phellodendron bark ethanol extracting solution as a raw material, carrying out enzymolysis with composite enzymes to obtain a precipitate, carrying out reflux extraction with ethanol, filtering to obtain a filtrate, and concentrating the filtrate under reduced pressure to recover ethanol to obtain a concentrated solution; and enriching the concentrated solution by a macroporous adsorbent resin process, eluting to obtain an eluate, concentrating the eluate, crystallizing and drying to obtain the obakulactone product. The yield of the obtained obakulactone is up to 0.35% (relative to the phellodendron raw material) or so, and the content is up to 80% above. The method can comprehensively utilize the phellodendron resources, changes wastes into valuable substances, reduces the environmental pollution, has the advantages of simple separation technique, high recovery rate and high limonin content, does not use any toxic organic solvent, is environment-friendly and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing phellodendron lactone from the waste liquid after extracting berberine from cortex cortex, in particular to an enzyme treatment technology combined with a macroporous adsorption resin separation technology from the waste liquid after berberine is extracted from phellodendron bark A method for preparing phellodactone. Background technique [0002] Phellodendron amurense Rupr., also known as limonin, limonene, and dictamnus lactone, is a triterpenoid bitter substance, mainly derived from the bark of Phellodendron amurense Rupr. leather etc. It has been proved to have anthelmintic, anti-ulcer, hypoglycemic effects, and can also relax the intestines, shorten the sleep time of mice caused by α-chloralose and urethane, and other physiological effects. Phellodendron Phellodendron is a traditional medicinal material in Chinese medicine. It has the functions of clearing heat and dampness, purging fire and steami...

AI Technical Summary

Problems solved by technology

At present, in the process of extracting berberine from phellodendron bark, the phellodendron lactone is not separated and utilized, thus wasting a lot of resources and causing great pollution to the environment

After research, it is found that the waste liquid after extracting berberine from Phellodendron bark contains a large amount of Phellodendron lactones. In the previous process, this part of the liquid was discarded, which not only polluted the environment, but also greatly wasted Phellodendron cortex resources.