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Siderophores-producing bacillus and applications thereof

A Bacillus and siderophore technology, applied in the field of siderophore Paenibacillus, can solve the problems of the improvement and growth promotion of Paenibacillus, and achieve the effects of increasing plant biomass, increasing content and improving iron nutrition

Active Publication Date: 2015-01-07
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many reports on the improvement of plant iron nutrition and growth promotion of siderophore-producing Pseudomonas and fungi, but there are no reports on the improvement of plant iron nutrition and growth promotion of Paenibacillus.

Method used

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  • Siderophores-producing bacillus and applications thereof
  • Siderophores-producing bacillus and applications thereof
  • Siderophores-producing bacillus and applications thereof

Examples

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Embodiment 1

[0025] A siderophore-producing Paenibacillus, the strain is classified and named as Paenibacillus illinoisensis, preserved by: General Microorganism Center (CGMCC), China Committee for Culture Collection of Microorganisms, address: 1 Beichen West Road, Chaoyang District, Beijing No. 3 Courtyard, date of deposit: August 29, 2014, deposit number: CGMCC No.9620.

[0026] CGMCC9620 screening process:

[0027] (1) Separation and purification

[0028] The strain was isolated and screened from peanut rhizosphere soil.

[0029] Soil samples were collected from a peanut planting site in Zhaiziwa (35°44'N, 116°75'E), Yanzhou City, Shandong Province. Carefully shake off the peanut rhizosphere soil into a sterile bag, put it in a low-temperature fresh-keeping box, and process it immediately after bringing it back to the laboratory. Weigh 10 g of the rhizosphere soil sample, put it into 90 mL of sterile water, and shake it for 30 min to make a soil suspension. Take 1mL of the suspensio...

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Abstract

The invention discloses a siderophores-producing bacillus. The strain is paenibacillus illinoisensis preserved in China General Microbiological Culture Collection Center (CGMCC) on August 29, 2014, wherein the address of CGMCC is Courtyard No.3, No. 1 of Beichen West Road, Chaoyang District, Beijing, and the Preservation No. is CGMCC No. 9620. The strain of the siderophores-producing bacillus can produce siderophores in an iron-limited culture medium. Under the conditions of pots and fields, after a bacterium suspension of the siderophores-producing strain of the siderophores-producing bacillus is subjected to seed soaking treatment before peanut seeding and is subjected to root-irrigation treatment in the growth period; in calcareous soil, the strain can obviously improve the iron nutrition of peanuts in the growth period, and can increase the chlorophyll content of peanuts, enhance the root activity of peanuts, promote the accumulation of nitrogen, phosphorus and potassium in peanut plant bodies, increase the biomass of plants, improve the quality of peanuts and increase the yield of peanuts.

Description

technical field [0001] The invention relates to a siderophore Paenibacillus and application thereof. Background technique [0002] Peanut is an important oil crop and economic crop in China, and northern China is one of the largest peanut cultivation areas. In recent years, the occurrence of iron-deficiency chlorosis in peanuts has become more and more serious, which has caused great damage to the yield and quality of peanuts. At present, people mainly rely on agricultural control and chemical control methods to prevent and control peanut iron deficiency yellowing disease, but agricultural control methods are time-consuming and laborious, and it is difficult to effectively prevent the occurrence of diseases from the root cause. Human health also has certain harm. Therefore, the use of biological methods to prevent and control the occurrence of peanut iron-deficiency yellowing has gradually become a hot spot of concern, and the key is to find strain resources that have a go...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 丁延芹杜秉海姚良同刘娣刘凯王文铜
Owner SHANDONG AGRICULTURAL UNIVERSITY
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