Method for preparing swine O-type foot-and-mouth disease synthetic peptide antigen 2700 by solid-phase fragment method

Abstract

The invention provides a method for preparing a synthetic peptide antigen 2700 of a swine O-type foot and mouth disease through a solid-phase fragment process. The method comprises the steps of with resin as a starting raw material, sequentially connecting 9-fluorenylmethoxycarbonyl protective amino acid to prepare a protected peptide fragment, meanwhile sequentially removing a Fmoc group, carrying out linker reaction by using a condensing agent, and cutting off the protected peptide fragment by using dilute acid or weak acid; connecting the fragment and 4-(4'-dimethoxy-9-fluorenylmethoxyaminomethyl)-phenyloxymethyl resin step by step, and then, connecting the fragment and a T helper cell epitope; removing a protecting group and the resin by using concentrated acid or strong acid to obtain a crude peptide of the synthetic peptide antigen 2700; and purifying by using ion exchange resin and a tangential flow film packaging system, and removing small molecules and salt through a concentration system to obtain the synthetic peptide antigen 2700. The method provided by the invention has the characteristics of stable process, short production period, convenience in obtaining raw and auxiliary materials, few wastewater, waste gas and waste residues, low production cost, high yield and the like.

Description

technical field

[0001] The invention relates to a method for preparing swine O-type foot-and-mouth disease synthetic peptide antigen 2700 by a solid-phase fragment method. Background technique

[0002] The structural formula of pig type O foot-and-mouth disease synthetic peptide antigen 2700 is as follows:

[0003]

[0004] Pig foot-and-mouth disease is an acute, febrile, highly contagious infectious disease caused by foot-and-mouth disease virus. In recent years, foot-and-mouth disease has broken out in a large area in Jiangsu, Sichuan, the three northeastern provinces and Inner Mongolia, causing huge economic losses. Currently, immunization remains the main means of controlling the disease. The traditional inactivated vaccine is still the leading method for the prevention and treatment of foot-and-mouth disease at home and abroad, but it is easy to cause side effects such as allergies, and there are also problems in biosafety. Therefore, a lot of research has been do...

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