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Use of polypeptide zl-m-1 in the preparation of anti-hepatocellular carcinoma drugs

A ZL-M-1, anti-liver cancer technology, applied in the field of biomedicine, can solve the problems of poor stability of polypeptides to proteasomes, high toxicity of polypeptides, and high cost of production and synthesis of polypeptides

Active Publication Date: 2016-06-01
JILIN UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the transformation of polypeptides with natural activity has become a research hotspot, but there are three bottlenecks in the development of polypeptides at this stage: (1) The production and synthesis costs of polypeptides are high; (2) The toxicity of polypeptides is relatively high; Poor proteasome stability

Method used

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  • Use of polypeptide zl-m-1 in the preparation of anti-hepatocellular carcinoma drugs
  • Use of polypeptide zl-m-1 in the preparation of anti-hepatocellular carcinoma drugs
  • Use of polypeptide zl-m-1 in the preparation of anti-hepatocellular carcinoma drugs

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Experimental program
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Effect test

Embodiment Construction

[0011] 1. The effect of ZL-M-1 on the growth of human liver cancer cells SMMC7721 in vitro

[0012] Human liver cancer cell SMMC7721 in the logarithmic growth phase was taken, and the cell concentration was adjusted to 5×10 with RPMI-1640 medium containing 10% fetal bovine serum. 4 cells / ml, add 100 μl of cell suspension in a 96-well culture plate, and add different concentrations of ZL-M-1 diluted with culture medium, (20 μl / well; final concentrations are: 0, 2, 4, 8, 10 , 12, 14, 16μg / ml), 37°C, 5% CO 2 After culturing for 24, 48, and 72 hours respectively, aspirate the supernatant, add 80 μl of fresh culture medium to each well, then add 20 μl of 5 mg / ml MTT to each well, continue to cultivate for 4 hours, centrifuge, aspirate the supernatant, add 150 μl DMSO to each well, lightly Gently shake to dissolve the formazan completely, and measure the absorbance with a microplate reader at a wavelength of 490nm. The cell viability was calculated according to literature, cell vi...

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Abstract

The invention belongs to the technical field of biomedicines and in particular relates to activity and application of anti-tumor polypeptide. According to the application, melittin is modified, the amino acid of amino acid is shortened, and the original instable amino acid proline is replaced into lysine with positive charges, so that the stability of melittin is improved, the central activity part of the N end of melittin is maintained, and polypeptide ZL-M-1 with relatively high anti-tumor activity is obtained by using a solid phase chemical synthesis method. The in-vitro / vivo pharmacodynamic experiment result shows that the polypeptide ZL-M-1 can remarkably inhibit the growth of human liver cancer cell SMMC7721 tumor, can increase secretion of in-vivo IL-2 of mice with tumor, and can be used as a medicine for anti-tumor treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to an immunomodulatory polypeptide and its application. The polypeptide of the invention has anti-liver cancer activity. Background technique [0002] Bee venom is a kind of transparent venom with aromatic smell secreted by the venom glands and accessory glands of worker bees. It is stored in the poison sac and discharged from the sting needle when it stings. Its chemical composition is relatively complex, containing carbon, hydrogen, sulfur, phosphorus, calcium, chlorine, nitrogen and other elements, and it is acidic, including polypeptides, enzymes and non-peptide substances. The main active ingredients of bee venom are melittin, melittin neuropeptide, phospholipase A2, hyaluronidase, dopamine and histamine. Studies have shown that bee venom is a complex mixture with high pharmacological and biological activities. Among them, melittin is the main active ingredient ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/10A61P35/00
Inventor 王文加赵磊
Owner JILIN UNIV
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