Primers and method for rapidly identifying group B streptococcus and detecting drug resistance thereof and primer application

Abstract

The invention relates to the biotechnical field, in particular to primers and a method for rapidly identifying group B streptococcus and detecting drug resistance thereof and primer application. Four pairs of specific primers are as follows: a cfb gene primer pair cfb-F and cfb-R for identifying the group B streptococcus; an ermB gene primer pair ermB-F and ermB-R for detecting the drug resistance of the group B streptococcus to macrocyclic lactones; an mefA / E gene primer pair mefA / E-F and mefA / E-R for detecting the drug resistance of the group B streptococcus to macrocyclic lactones; an linB gene primer pair linB-F and linB-R for detecting the drug resistance of the group B streptococcus to lincosamides. The method comprises the following steps: Step 1, DNA extraction; Step 2, primer design; Step 3, gene amplification; Step 4, result interpretation. According to the invention, the primers can identify GBS (group B streptococcus) in a genital tract of a pregnant woman and detect the drug resistanceof the GBS to erythromycin and clindamycin at the same time so as to guide clinical medication.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer and a method for rapidly identifying group B streptococcus and detecting its drug resistance and the application of the primer. Background technique [0002] Group B Streptococci (GBS), also known as Streptococcus agalactiae, can cause neonatal pneumonia, meningitis, sepsis and other diseases. Newborns with GBS infection are closely related to GBS colonization of the mother's reproductive tract. Guidelines from the Centers for Disease Control and Prevention (CDC) recommend screening for GBS colonization and antibiotic prophylaxis for pregnant women at 35-37 weeks of gestation. Penicillin is the first choice drug. According to literature reports, 12% of pregnant women are allergic to penicillin. If the pregnant women allergic to penicillin are sensitive to clindamycin drug sensitivity test, the US CDC recommends clindamycin as an alternative drug for pregnant women allergic ...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many types of microbial flora in the reproductive tract of pregnant women and the environment is complex. The traditional GBS detection method has low specificity and low sensitivity.

In the prior art, the detection method for group B streptococcus mainly relies on the conventional bacteriological culture method, and the detection generally takes 2 to 3 days, which has the disadvantages of cumbersome operation and long time-consuming; the drug resistance detection of group B streptococcus Relying on the results of the drug-sensitive paper test, this method takes a long time to test, and is greatly affected by human factors, the quality of the medium, and the accuracy and uniformity of the drug-sensitive paper, and has cumbersome operations, and is insufficient in speed and accuracy. and other shortcomings

Therefore, the existing technology is difficult to effectively carry out prenatal GBS screening and antibiotic prophylaxis for pregnant women

[0004] In addition, there is only a molecular biology kit for identifying GBS in the prior art, that is, the prior art uses the molecular biology kit for GBS to identify GBS, but the prior art does not identify GBS in the process of identifying GBS. Cannot simultaneously detect drug resistance in GBS

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