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Lactobacillus freeze-drying protective agent

A technology of freeze-drying protective agent and lactic acid bacteria, which is applied in the direction of bacteria, preserved microorganisms, microorganisms, etc., which can solve the problems of limited application, poor external resistance, difficulty in preparation and long-term storage, so as to improve protection ability, speed up recovery and prevent frostbite and the effect of moisture loss

Inactive Publication Date: 2015-02-11
ZHENJIANG BIO INNOVA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lactic acid bacteria have high requirements on nutrition and environmental conditions, and have poor resistance to the outside world, making it difficult to prepare and store for a long time, which severely limits their application in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The composition and content of the lactic acid bacteria lyoprotectant of the present embodiment are as follows:

[0016] name Weight (g) ectoine 0.8 Trehalose 10 skim milk 25 sodium glutamate 2

[0017] The effect of the lactic acid bacteria freeze-drying protective agent of the present embodiment is as follows:

[0018] Add the above-mentioned freeze-drying agent to the centrifuged Lactobacillus bulgaricus sludge in a ratio of 1:5, mix evenly, and put it into a freeze dryer to freeze-dry.

[0019] The bacteria slime of the control group was not added with a freeze-drying protective agent, and was freeze-dried in a freeze-dryer at the same time. The temperature of the cold trap was -60 degrees, the vacuum degree was 20 Pa, and it was frozen for about 30-35 hours to completely dry, and the freeze-dried powder of the bacteria was obtained. The number of live bacteria was detected by plate counting method, and the number of live bact...

Embodiment 2

[0022] The composition and content of the lactic acid bacteria lyoprotectant of the present embodiment are as follows:

[0023] name Weight (g) ectoine 1.5 Trehalose 10 skim milk 20 sodium glutamate 1

[0024] The effect of the lactic acid bacteria freeze-drying protective agent of the present embodiment is as follows:

[0025] Add the above-mentioned freeze-drying agent to the centrifuged Streptococcus lactis sludge at a ratio of 1:5, mix evenly, and put it into a freeze-dryer to freeze-dry.

[0026] The bacteria slime of the control group was not added with a freeze-drying protective agent, and was freeze-dried in a freeze-dryer at the same time. The temperature of the cold trap was -60 degrees, the vacuum degree was 20 Pa, and it was frozen for about 30-35 hours to completely dry, and the freeze-dried powder of the bacteria was obtained. The number of viable bacteria was detected by plate counting method, and the number of viable bac...

Embodiment 3

[0029] The composition and content of the lactic acid bacteria lyoprotectant of the present embodiment are as follows:

[0030] name Weight (g) ectoine 3 Trehalose 8 skim milk 20 sodium glutamate 0.5

[0031] The effect of the lactic acid bacteria freeze-drying protective agent of the present embodiment is as follows:

[0032] Add the above-mentioned freeze-drying agent to the bifidobacteria sludge obtained by centrifugation at a ratio of 1:5, mix evenly, and put it into a freeze dryer for freeze-drying.

[0033] The bacteria slime of the control group was not added with a freeze-drying protective agent, and was freeze-dried in a freeze-dryer at the same time. The temperature of the cold trap was -60 degrees, the vacuum degree was 20 Pa, and it was frozen for about 30-35 hours to completely dry, and the freeze-dried powder of the bacteria was obtained. The number of viable bacteria was detected by plate counting method, and the number of...

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Abstract

The invention provides a lactobacillus freeze drying protective agent, which comprises 0.1-4% of ectoine, 2-8% of mycose, 10-20% of skimmed milk and 0.1-2% of monosodium glutamate. The protective agent can greatly increase the live bacteria viability amount of lactobacillus during a freeze-drying processing process. The agent has the characteristics of convenient usage, obvious effect, no pollution and environment protection. By using the freeze-drying process for processing lactobacillus by the protective agent, the lactobacillus freeze-drying powder live bacteria amount can reach more than 1*1011CFU / g.

Description

technical field [0001] The invention relates to a bacterial freeze-drying protectant, in particular to a lactic acid bacteria freeze-drying protectant. Background technique [0002] Lactic acid bacteria is a general term for bacteria that can ferment carbohydrates and mainly produce lactic acid. With the continuous deepening of research on the mechanism of beneficial effects of lactic acid bacteria on human health and the enhancement of people's awareness of health care, lactic acid bacteria have received more and more attention and research and development, and have been widely used in nutrition and health care, food, medicine, feed additives and other industries. . [0003] Lactic acid bacteria have high requirements on nutrition and environmental conditions, and have poor resistance to the outside world, making it difficult to prepare and store for a long time, which severely limits their application in actual production and life. At present, lactic acid bacteria are m...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/225C12R1/46C12R1/01
CPCC12N1/20C12N1/04
Inventor 朱道辰李静静何晓成陈焱
Owner ZHENJIANG BIO INNOVA BIOTECH
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