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Production technology of bile salt hydrolase-yielding lactobacillus paracasei dry powder living bacterium preparation

A technology of bile salt hydrolyzing enzyme and live bacteria preparation, which is applied in the direction of bacteria, microorganisms, preserved microorganisms, etc., and can solve the problems that have not been reported in relevant literature and patent reports at home and abroad

Inactive Publication Date: 2015-02-18
BEIJING BEINONG HUIBANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, regarding the preparation of live bacterial preparations using Lactobacillus paracasei KL1-Liu, which is isolated and screened from the bile salt hydrolysis enzyme in Tibetan mushrooms, the use of high-density fermentation in automatic fermenters, and the use of rapeseed peptone to completely replace the nitrogen in the improved MRS medium The source method has not yet been seen in relevant literature reports and patent reports at home and abroad

Method used

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  • Production technology of bile salt hydrolase-yielding lactobacillus paracasei dry powder living bacterium preparation
  • Production technology of bile salt hydrolase-yielding lactobacillus paracasei dry powder living bacterium preparation
  • Production technology of bile salt hydrolase-yielding lactobacillus paracasei dry powder living bacterium preparation

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Embodiment 1

[0035] Embodiment 1, the small-scale production technology of bile salt hydrolysis enzyme Lactobacillus paracasei KL1-Liu live bacterial preparation

[0036] 1. Activation and expansion of strains

[0037] The Lactobacillus paracasei KL1-Liu strain kept in the MRS test tube was cultured at 37°C for 16 hours with the improved MRS culture, activated for 2 to 3 generations, and refrigerated for later use; the activated bacterial solution was transplanted to 501 Rapeseed peptone-modified MRS medium was cultured at 37°C for 16 hours to obtain an expanded culture medium.

[0038] 2. Determination of fermentation medium

[0039] (1) Rapeseed peptone of different quality instead of partial nitrogen source test of improved MRS

[0040] Use 501 rapeseed peptone, 502 rapeseed peptone and 503 rapeseed peptone to replace the tryptone in the improved MRS medium, transplant the activated strains into the above medium at 4% inoculum, and inoculate at 34°C After 16 hours of fermentation, th...

Embodiment 2

[0068] Embodiment 2, the pilot-scale production technology of bile salt hydrolysis enzyme Lactobacillus paracasei KL1-Liu live bacterial preparation

[0069] 1. Pilot fermentation verification test

[0070] According to the optimization results of the orthogonal test, the pilot fermentation verification test was carried out. In a 50L automatic fermentation tank, 25L501 rapeseed peptone modified MRS was used as the fermentation medium, and 20% sterilized Na 2 CO 3 The solution controls the pH value of the fermentation broth, and the stirring speed is controlled at 140r / min. The improved MRS medium was used as the fermentation medium, and the pH of the fermented liquid was not controlled (natural) as the control group. MRS medium was used to detect the number of viable bacteria in the fermentation broth by the pour plate culture method, and each dilution was repeated three times. The results are shown in Table 6.

[0071] Table 6 Pilot fermentation verification test results

...

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Abstract

The invention relates to a preparation method of a bile salt hydrolase-yielding lactobacillus paracasei dry powder living bacterium preparation, wherein the preparation method is suitable for the production of functional probiotic lactic acid bacterium leavening agents and microecologics for eating or feeding. The method comprises the following steps: carrying out activation, cultivation enlargement, high-density fermentation and centrifugal concentration on bile salt hydrolase-yielding lactobacillus paracasei KL1-Liu CGMCC No.7029 screened from Tibetan kefir, adding a freeze-drying protective additive, pre-freezing, freeze-drying and the like. According to the method, a nitrogen source in an improved MRS culture medium is completely replaced with rapeseed peptone, so that the production cost is lowered; and production and fermentation conditions and a formula of the freeze-drying protective additive are optimized, so that the number of living bacteria of the KL1-Liu strain can reach above 10<10>CFU / g, and the survival rate can reach above 85%. The prepared living bacterium preparation is low in cost and high in living bacterium content, and is a high-quality probiotic living bacterium preparation / leavening agent with a serum cholesterol lowering function.

Description

technical field [0001] The invention relates to a production technology of bile-salt hydrolysis enzyme Lactobacillus paracasei KL1-Liu dry powder preparation, which is suitable for functional probiotic lactic acid bacteria starter, edible or feeding microecological preparation, functional food, and functional fermented food production. Background technique [0002] The Lactobacillus paracasei (Lactobacillus paracasei) KL1-Liu involved in the present invention is a lactic acid bacterium that produces bile salt hydrolysis enzyme and has the effect of lowering serum cholesterol, which is isolated and screened from Tibetan mushroom. [0003] "Tibetan Kefir" (Tibetan Kefir, TK), also known as Kefir grains, was discovered by humans in the 19th century. Kefir grains originated in the North Caucasus region of Russia. Local herdsmen poured cow's milk or goat's milk into sheepskin pockets for natural fermentation to produce yoghurt. The residue was added with cow's or goat's milk to ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20C12N1/04
Inventor 刘慧张红星熊利霞谢远红高秀芝金君华
Owner BEIJING BEINONG HUIBANG BIOTECH
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