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Substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide

A technology of chemiluminescence and hydrogen peroxide, applied in the field of microfluidic analysis, to achieve the effect of good hydrophilicity, low price and easy preparation

Inactive Publication Date: 2015-02-18
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, sensing methods combining chemiluminescence detection and bucky microfluidics have not been reported yet.

Method used

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  • Substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide
  • Substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide
  • Substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide

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Embodiment

[0046] A substrate pre-immobilized cloth-based microfluidic chemiluminescence method for detecting hydrogen peroxide, comprising the following steps:

[0047] (1) Fabrication of bucky microfluidic chip

[0048] a) Pattern design of chemiluminescence chip

[0049] The chip pattern is separated by the sample injection area (4mm×4mm) and the detection area (diameter 8mm), and connected by microchannels (width: 3mm, 4mm, 5mm; length: 5mm, 10mm, 15mm) (such as figure 2 shown). The advantages of such chemiluminescent chip pattern design include: (1) the fluid flow is driven by the capillary force of the cloth fiber to realize flow injection chemiluminescence; On the one hand, it is convenient for sample injection, and can realize automatic sample injection without pollution; (3) Utilize the time interval when the solution flows from the sample injection area to the detection area to flexibly realize the cloth-based microfluidic chemiluminescence CCD image acquisition.

[0050] b) ...

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Abstract

The invention discloses a substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide. The substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide comprises the following steps: manufacturing a fabric-based micro fluid control chip; dropwise adding a chemiluminiscence base solution to a detection zone of the chip, then airing at the temperature of 2-8 DEG C in a dark place, and storing; dropwise adding a sample solution to a sample introducing zone of the chip, if a sample contains H2O2, triggering chemiluminiscence reaction, acquiring a luminescence image by virtue of CCD (Charge Coupled Device) digital imaging equipment, and processing the luminescence image by virtue of software Matlab and Image J. A chemiluminiscence system of the substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide has the advantages of good stability, high detection sensitivity, wide dynamic range and the like and can be used for directly or indirectly determining hydrogen peroxide, so that the substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide has extremely important research significance in the fields of environmental monitoring, food safety testing, biological chemistry, clinical disease diagnosis and the like.

Description

technical field [0001] The invention belongs to the field of microfluidic analysis, and in particular relates to a substrate pre-immobilized cloth-based microfluidic chemiluminescence method for detecting hydrogen peroxide. Background technique [0002] Hydrogen peroxide is an important substance in biological systems, which seriously affects cell metabolism, and is also accompanied by the production and consumption of hydrogen peroxide in many enzymatic reactions, protein accumulation and antigen-antibody recognition. In the process of food processing, some unscrupulous merchants often use cheap industrial hydrogen peroxide to sterilize and bleach food to make the food look better. However, the human body's ingestion of industrial hydrogen peroxide will cause various harms and will make the human body resistant to Decreased, induced gene mutation, causing the occurrence of a variety of diseases in the stomach, heart and lungs and other parts. At present, the state has bann...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 章春笋管文荣
Owner SOUTH CHINA NORMAL UNIVERSITY
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