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A kind of high borneol content borneol camphor tissue culture breeding method

A technology of borneol camphor content and borneol camphor content is applied in the field of borneol camphor camphor tissue culture and breeding with high borneol content, and can solve the problems such as the lack of clear explant material borneol content and the like.

Inactive Publication Date: 2017-05-17
JIANGXI ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The paper did not clarify the borneol content of the explant material

Method used

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  • A kind of high borneol content borneol camphor tissue culture breeding method
  • A kind of high borneol content borneol camphor tissue culture breeding method
  • A kind of high borneol content borneol camphor tissue culture breeding method

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Experimental program
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Embodiment 1

[0026] a, the establishment of sterile line: with the high-content borneol camphor tree of 90%~97% borneol content in the leaf essential oil, the year-old branches are used as explants, the leaves and terminal buds are cut off, and the lower part of the terminal buds contains 4~5 For the stem section of an axillary bud, clean the surface with detergent, soak it in alcohol for 30 seconds on the ultra-clean workbench, and then sterilize it with 5% sodium hypochlorite solution for 6 minutes, then sterilize it with 0.1% mercuric chloride for 5-6 minutes, and then sterilize it with Rinse 5-6 times with sterile water, cut off about 0.5 cm at both ends of the stem segment, and cut the stem segment into small segments with 1-2 axillary buds depending on the length of the internodes, inoculate them in the induction medium, and grow them under light intensity of 2000-2000 3000Lux, 12h / day, culture at 20-30℃. After about 1 week of cultivation, the axillary buds on the stems begin to expan...

Embodiment 2

[0032] a, the establishment of sterile line: with the high-content borneol camphor tree of 90%~97% borneol content in the leaf essential oil, the year-old branches are used as explants, the leaves and terminal buds are cut off, and the lower part of the terminal buds contains 4~5 For the stem section of an axillary bud, clean the surface with detergent, soak it in alcohol for 30 seconds on the ultra-clean workbench, and then sterilize it with 5% sodium hypochlorite solution for 6 minutes, then sterilize it with 0.1% mercuric chloride for 5-6 minutes, and then sterilize it with Rinse 5-6 times with sterile water, cut off about 0.5 cm at both ends of the stem segment, and cut the stem segment into small segments with 1-2 axillary buds depending on the length of the internodes, inoculate them in the induction medium, and grow them under light intensity of 2000-2000 3000Lux, 12h / day, culture at 20-30℃. After about 1 week of cultivation, the axillary buds on the stems begin to expan...

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Abstract

The invention discloses a tissue-culture breeding method for high-borneol-content cinnamomum camphora. The tissue-culture breeding method comprises: selecting and culturing a cinnamomum camphora individual with the borneol content of 90% or more from cinnamomum camphora natural variation plant, acquiring a semi-lignification stem segment with annual sprouting as an explant, performing disinfection processing, establishing an aseptic line, and then forming a regenerated plant through bud primary induction, propagation culture, rooting culture and the like. Proper formulas and culture conditions of all links for tissue-culture breeding of high-borneol-content cinnamomum camphor are screened out, and the high-borneol-content cinnamomum camphor regenerated plant is successively obtained. Therefore, the method effectively solves the technical problems that browning and dead seedlings are generated during tissue-culture breeding of cinnamomum camphor with the borneol content of 90% or more, and provides seedling guarantee for construction of an excellent cinnamomum camphor raw material forest base.

Description

[0001] Technical field: [0002] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture and breeding of borneol camphor with high borneol content. [0003] Background technique: [0004] Borneocamphora is a chemical type rich in natural d-borneol (natural borneol) in the branches and leaves found in camphor tree (Cinnamonun Camphora (I) Presl), which can be processed into natural D-borneol. Natural borneol is a kind of precious traditional Chinese medicine and high-grade spice. It has antibacterial, anti-inflammatory, astringent, muscle-promoting, anti-pregnancy, and pain-relieving effects. It has broad application prospects in the pharmaceutical and spice industries. [0005] The half-siblings bred from free-pollinated seeds of borneol camphor have extremely serious differentiation. Among them, excellent individuals with high leaf essential oil content and borneol content of the main component in the essential oil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 江香梅戴小英章挺肖复明杨海宽汪信东伍艳芳邱凤英宋晓琛王召滢
Owner JIANGXI ACAD OF FORESTRY
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