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A kind of collagen-based biomaterial and preparation method thereof

A technology of biomaterials and collagen, applied in animal/human proteins, connective tissue peptides, specific peptides, etc., can solve problems such as inability to stop bleeding, wound repair, and low water absorption

Inactive Publication Date: 2017-02-08
重庆紫郡农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Conventional collagen-based biomaterials have low water absorption and cannot be used for hemostasis and wound repair

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Take the mackerel skin, cut the mackerel skin into pieces, pre-freeze it, and then freeze-dry it. After freeze-drying, crush the mackerel skin with a pulverizer, and use 15wt% acetic acid solution for microwave stirring extraction. The mackerel The weight ratio of skin powder to 15wt% acetic acid solution is 1:9, the extraction temperature is 75°C, the microwave power is 150w, the extraction time of Spanish mackerel skin powder is 5h, and the extract is centrifuged with a high-speed centrifuge, the centrifuge speed 5000rpm, centrifugation time 20min, take the supernatant, adjust the supernatant to pH 5.5 with sodium hydroxide, place it at 4°C for 24h, filter with a filter cloth, collect the filter residue, and vacuum-dry the filter residue to obtain Spanish mackerel Skin collagen; (2) Take the eel skin, cut the eel skin into pieces, pre-freeze it first, and then freeze-dry it. The weight ratio of powder to 7wt% acetic acid solution is 1:8, the extraction temperature...

Embodiment 2

[0030] (1) Take the mackerel skin, cut the mackerel skin into pieces, pre-freeze the mackerel, and then freeze-dry. The weight ratio of skin powder to 8wt% acetic acid solution is 1:5, the extraction temperature is 65°C, the microwave power is 300w, the extraction time of Spanish mackerel skin powder is 2h, and the extract is centrifuged with a high-speed centrifuge, the centrifuge speed 3500rpm, centrifugation time 20min, take the supernatant, adjust the supernatant to pH 4.5 with sodium hydroxide, place it at 4°C for 24h, filter with a filter cloth, collect the filter residue, and vacuum dry the filter residue to obtain Spanish mackerel Skin collagen; (2) Take the eel skin, cut the eel skin into pieces, pre-freeze it, and then freeze-dry it. The weight ratio of powder to 4wt% acetic acid solution is 1:4, the extraction temperature is 60°C, the eel skin powder extraction time is 3h, and the extract is centrifuged with a high-speed centrifuge, the centrifuge speed is 5000rpm, ...

Embodiment 3

[0032] (1) Take the mackerel skin, cut the mackerel skin into pieces, pre-freeze the mackerel, and then freeze-dry. The weight ratio of skin powder to 8wt% acetic acid solution is 1:5, the extraction temperature is 65°C, the microwave power is 300w, the extraction time of Spanish mackerel skin powder is 2h, and the extract is centrifuged with a high-speed centrifuge, and the centrifuge speed is 3500rpm, centrifugation time 20min, take the supernatant, adjust the supernatant to pH 5 with sodium hydroxide, place it at 4°C for 24h, filter with a filter cloth, collect the filter residue, and vacuum dry the filter residue to obtain Spanish mackerel Skin collagen; (2) Take the eel skin, cut the eel skin into pieces, pre-freeze it, and then freeze-dry it. The weight ratio of powder to 4wt% acetic acid solution is 1:4, the extraction temperature is 60°C, the eel skin powder extraction time is 3h, and the extract is centrifuged with a high-speed centrifuge, the centrifuge speed is 5000...

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PUM

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Abstract

The invention belongs to the field of biological materials, and discloses a collagen-based biological material and a preparation method thereof. The collagen-based biological material comprises the following raw materials: 15-25 parts of spanish mackerel skin collagen, 6-13 parts of eel skin collagen, 4-10 parts of locust bean gum, 5-11 parts of fructosan and 12-20 parts of gelatin. The preparation method comprises the following steps: (1) cutting spanish mackerel skin into pieces, freeze-drying, crushing, stirring and extracting with an acetic acid solution by a microwave, centrifugally separating, adjusting the pH, standing, filtering and drying into the spanish mackerel skin collagen; (2) cutting eel skin into pieces, freeze-drying, crushing, stirring and extracting with an acetic acid solution, centrifugally separating, taking supernatant fluid, adjusting the pH, standing, filtering, collecting filter residues, and drying to obtain the eel skin collagen; and (3) adding the spanish mackerel skin collagen, the eel skin collagen, locust bean gum, fructosan and gelatin to hot water, stirring evenly, and drying to prepare the collagen-based biological material.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to a biological material and a preparation method thereof, in particular to a collagen-based biological material and a preparation method thereof. Background technique [0002] Collagen is the main protein component of all connective tissues in animals—skin, tendon, ligament, cartilage, etc. It is one of the most abundant proteins, accounting for 30% of all proteins in mammals. Due to the special properties of collagen and its advantages in nature, collagen has a very broad application prospect in the field of biomedicine. Native collagenous tissues have high strength, but when collagen-based devices are prepared from soluble collagen, the strength is low. Therefore, cross-linking is usually used to introduce new chemical bonds between collagen molecules, so that the product can obtain appropriate strength for specific situations. Another purpose of cross-linking is to reduce the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K1/30A61L15/32A61L15/28
Inventor 胡永坤
Owner 重庆紫郡农业发展有限公司