Bacillus amyloliquefaciens and application thereof

A technology for dissolving starch spores and bacilli, applied in the field of agricultural biology, can solve problems such as insufficient safety and effectiveness

Active Publication Date: 2015-02-25
BEIJING LYUHUAN INT SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of not being safe and effective when the chemical medicine soaking method is used to prevent and control fruit fungal diseases, and to provide a safer and more effective method for microbial biological control of fruit fungal diseases

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Prepare medium, the composition is the yeast extract of 5g / L, the peptone of 10g / L and the sodium chloride of 10g / L.

[0034] Bacillus amyloliquefaciens with the preservation number CGMCC NO.8040 was inoculated and cultured in the above-mentioned medium until the cell concentration was 10 9 CFU / mL to obtain bacterial agent. Under the microscope, the typical optical micrograph of the strain is as follows figure 1 shown.

Embodiment 2

[0036] Use the Bacillus amyloliquefaciens strain with the preservation number CGMCC NO.8040 to treat Botrytis cinerea, Penicillium expansum, Botrytis cinerea, and Penicillium expansum (Link)Thom), Gloeosporium piperatum, Monilinia fructicola, Monilinia laxa, Monilinia fructigena, Monilinia fructicola, The antibacterial effects of Penicillium digitatum and Penicillium italicum were tested, and the results shown in Table 1 were obtained. Among them, the typical inhibitory effect on botrytis cinerea is as follows figure 2 shown. The inhibitory effect on the size of peach brown rot lesions is shown in the figure image 3 shown. Among them, the peach on the left is the treatment with the Bacillus amyloliquefaciens of the present invention, and the peach on the right is the control without the Bacillus amyloliquefaciens of the present invention. The inhibitory effect on the size of tomato gray mold lesions is as follows: Figure 4 shown. The tomato peach on the left is the co...

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PUM

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Abstract

The invention discloses Bacillus amyloliquefaciens strain, the preservation number of the strain is CGMCC NO.8040. On the other hand, the invention also provides a bacteria agent, wherein the bacteria agent contains live bacteria of the Bacillus amyloliquefaciens strain and a culture medium. On the other hand, the invention also provides the application of the Bacillus amyloliquefaciens strain and / or bacteria agent in the prevention and treatment of fungal diseases of fruits. In another aspect, the invention also provides a method for prevention and treatment of fungal diseases of fruits, and the method includes contact of the bacteria agent of the live bacteria of the Bacillus amyloliquefaciens strain with the fruits. Through the technical proposal, the Bacillus amyloliquefaciens strain can effectively realize the prevention and treatment of fungal diseases of fruits, and is free of residue of harmful chemical substances.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a bacterial strain of Bacillus amyloliquefaciens, a bacterial agent containing the bacterial strain, uses of the bacterial strain and the bacterial agent, and a method for preventing and treating fruit fungal diseases. Background technique [0002] Fruit storage and preservation is the continuation of agricultural production. With the improvement of living standards, the public pays more and more attention to health and environmental issues, and the requirements for fruit quality are also getting higher and higher, and green food is popularized rapidly. Therefore, it is very important to find a safe and effective technology to preserve and sterilize the fruit after harvest. [0003] Fungal diseases are the main factors that induce fruit spoilage, so the prevention and control of fruit fungal diseases is crucial to the storage of fruits. Most of the current methods for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/07
CPCA23B7/155C12N1/20C12N1/205C12R2001/07
Inventor 全利红
Owner BEIJING LYUHUAN INT SCI & TECH
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