Rapid detection method of heavy metals and trace elements in crops by atomic fluorescence spectroscopy based on laser ablation plume

Abstract

The invention discloses a rapid atomic fluorescence spectroscopy heavy metal and trace element detection method based on laser ablation plume. The method comprises the following steps: cleaning a crop sample by using deionized water, and placing the cleaned sample on a sample table; forming 355nm laser by a first path of laser after passing through a three frequency generator, and after a light path is raised, focusing the laser at a position above the sample table to beat the surface of the sample to form the high-density plume; beating the high-density plume from the upward side of the sample by a second path of laser so as to enable atoms to be motivated; acquiring an atomic spectrum of the crop sample, and selecting four spectral lines which are self-absorptive, self-reversal and highest in strength from characteristic spectral lines according to to-be-tested heavy metal and trace elements; establishing a plurality of kinds of multiple regression models by using a sample reference value as output and the spectral line strength as input, selecting four optimal models from multiple regression models, and establishing a comprehensive model; and acquiring four spectral line strength values, inputting the four spectral line strength values into the comprehensive model aiming at the to-be-tested crop sample, and computing the contents of the heavy metal and trace elements of a crop.

Description

technical field [0001] The invention relates to a technology for detecting heavy metals and trace elements in crops, in particular to a fast detection method for heavy metals and trace elements in crops based on laser ablation plume atomic fluorescence spectroscopy. Background technique [0002] Heavy metals (cadmium, chromium, lead, copper, etc.) and trace elements (silicon, iron, boron, etc.) have an important impact on crop life activities. Under heavy metal stress, the content of proline in crops increases, photosynthesis and respiration are inhibited to varying degrees, and crop yields decrease or even fail. The trace elements are closely related to the growth status and yield of crops. For example, Si can improve the disease resistance of crops and promote growth; Fe is a component of various enzymes in plants, and Fe deficiency can lead to chlorosis. Obtaining the information of heavy metals and trace elements of crops is conducive to understanding the growth status...

AI Technical Summary

Problems solved by technology

However, these methods are complex and costly, and cannot reflect the distribution of heavy metals and trace elements on the plant surface

In recent years, laser-induced breakdown spectroscopy, which has received much attention and rapidly developed, can detect a variety of trace elements, but the signal-to-noise ratio is low, and the detection limit cannot meet the detection of some heavy metals and trace elements in crops.

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