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Method utilizing ultrasonic waves to extract paclitaxel from taxus chinensis

A paclitaxel and ultrasonic technology, applied in the direction of organic chemistry, can solve the problems of complicated process, high production cost, and low extraction rate of paclitaxel, and achieve the effect of less solvent usage, high utilization rate, and reduced production cost

Inactive Publication Date: 2015-03-11
朱艳玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for extracting paclitaxel from Taxus chinensis by ultrasonic waves, so as to solve the deficiencies in the prior art that the process is complicated, the production cost is high and the extraction rate of paclitaxel is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting paclitaxel from yew by ultrasonic wave, comprising the following steps:

[0024] (1) Raw material pretreatment: the branches and leaves of Chinese yew are dried at a temperature of 40°C until the moisture content is 8%, and then it is crushed into a 60-mesh powder;

[0025] (2) extract paclitaxel: add the solvent of 3 times of powder weight to the powder of the yew branches and leaves that step (1) makes, this solvent is that ethyl alcohol and acetone are mixed by weight ratio 10:8, stir to make powder Fully dissolve, then put the mixture of powder and solvent into a reflux extractor equipped with an ultrasonic oscillator, under the action of ultrasonic waves with an ultrasonic frequency of 28KHz and a power of 1400kw, heat to a temperature of 30°C and maintain a constant temperature for reflux extraction for 4 times, Each extraction time is 24min to obtain the extract;

[0026] (3) Adsorption: the extract in step (2) is filtered, and the filtra...

Embodiment 2

[0032] A method for extracting paclitaxel from yew by ultrasonic wave, comprising the following steps:

[0033] (1) Raw material pretreatment: the branches and leaves of Chinese yew are dried at a temperature of 50° C. until the water content is 12%, and then crushed into 200-mesh powder;

[0034] (2) extract paclitaxel: add the solvent of 3 times of powder weight in the powder of the yew branches and leaves that step (1) makes, this solvent is that ethanol ethyl ester and acetone are mixed in 10:12 by weight, stir to make powder Fully dissolve, then put the mixture of powder and solvent into a reflux extractor equipped with an ultrasonic oscillator, under the action of ultrasonic waves with an ultrasonic frequency of 32KHz and a power of 1500kw, heat to a temperature of 35°C and maintain a constant temperature for reflux extraction for 5 times, Each extraction time is 30min to obtain the extract;

[0035] (3) Adsorption: the extract in step (2) is filtered, and the filtrate ...

Embodiment 3

[0041] A method for extracting paclitaxel from yew by ultrasonic wave, comprising the following steps:

[0042] (1) Raw material pretreatment: the branches and leaves of Chinese yew are dried at a temperature of 45°C until the water content is 10%, and then crushed into 80-mesh powder;

[0043](2) extract paclitaxel: add the solvent of 3.5 times of powder weight to the powder of the yew branches and leaves that step (1) makes, this solvent is that ethyl alcohol ethyl ester and acetone are mixed by weight ratio 10:10, stir to make powder Fully dissolve, then put the mixture of powder and solvent into a reflux extractor equipped with an ultrasonic oscillator, under the action of ultrasonic waves with an ultrasonic frequency of 30KHz and a power of 1450kw, heat to a temperature of 32°C and maintain a constant temperature for reflux extraction for 4 times, Each extraction time is 28min to obtain the extract;

[0044] (3) Adsorption: the extract in step (2) is filtered, and the fi...

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Abstract

The invention discloses a method utilizing ultrasonic waves to extract paclitaxel from taxus chinensis. The method comprises the following steps: taxus chinensis branches and leaves are dried and then crushed, a vinegar naphtha and acetone mixed solution of which the weight is 3-4 times of that of the powder obtained through crushing to obtain a mixture, the mixer is thrown into a reflow extractor for extraction of 4-5 times to obtain an extract, the extract is filtered and then passes through macroporous resin columns, the obtained paclitaxel section is eluted by clear water and ethanol, the obtained crude paclitaxel and silica gel are mixed with methylene dichloride and then dried, the dried mixer is packed into chromatographic columns after being mixed with silica gel at the ratio of 1:(3-4), an ethyl acetate and acetone system is adopted for elution, the obtained paclitaxel section is concentrated and dried by distillation and then dissolved in ethanol and water, and filtering, crystallization and drying are performed after still cooling to obtain the paclitaxel end product. When the method is used for paclitaxel extraction, the extraction rate can reach 96-99%, the purity of paclitaxel in the product can reach 94-96%, and accordingly, remarkable economic benefits can be obtained.

Description

technical field [0001] The invention relates to the technical field of separating and extracting organic compounds from plants, in particular to a method for extracting paclitaxel from yew by using ultrasonic waves. Background technique [0002] Yew, also known as clover, peach pine, and torreya, is widely distributed all over the world. The whole plant is rich in harringtonine, epiharringtonine, demethyl harringtonine, and acetyl harringtonine , harringtonine, harringtonine, and paclitaxel, and the yew cream that uses the extracts of yew and its congener plant, Taxus brevifolia, contains a variety of ingredients: taxine, paclitaxel, taxine, and taxol . Among them, paclitaxel has the best anti-Ca and anti-TB activities, and it is the main anti-Ca and anti-TB components and main ingredients in the yew extract. [0003] Paclitaxel is also known as taxol, porphyrin, and special element, its chemical name is 5β, and its molecular formula is C47H51NO14. Paclitaxel is mainly ap...

Claims

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Application Information

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IPC IPC(8): C07D305/14
CPCC07D305/14
Inventor 朱艳玲卢连伟
Owner 朱艳玲