Method for preparing lactic acid bacterium granule capsule
A technology of granular capsules and lactic acid bacteria, applied in the field of lactic acid bacteria, can solve the problems of low cost of lactic acid bacteria products, low activity of bacteria strains, and insoluble bacteria particles in capsules, so as to reduce the risk of bacterial contamination, reduce the loss of bacterial activity, damage reduction effect
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Embodiment 1
[0028] Inoculate the Lactobacillus paracasei stored at –20℃ into the basal liquid medium, and adjust the glucose of the medium to a mixture of sucrose and mannitol with a mass ratio of 1:1. The strain was cultured in this medium for 5 After several times, the strain was stored at –20°C for later use;
[0029] Inoculate Lactobacillus paracasei stored at -20°C after acclimatization into the basal liquid medium, shake and cultivate for 8 hours at a speed of 70rpm / min to obtain the first-grade shake flask seed solution; the first-grade seed solution was mixed with 8% The inoculum was inoculated into the newly prepared MRS liquid medium, shaken and cultivated for 8 hours, and then the growth curve of the strain was measured at intervals of 30 minutes, and the time for the strain to reach the middle and early stages of the stable growth period was determined to be 25 hours. At this time, the culture was stopped. Obtain the seed liquid of the secondary shake flask; inoculate the seco...
Embodiment 2
[0036] The difference from Example 1 is:
[0037] The second layer of coating of granules: add the solid mixture formed by sterile solid cornstarch and lactose to the granules prepared in the first granulation in proportion, and the amount of sterilized solid cornstarch is the mass of wet sludge 3 times (W:W), the amount of lactose added is 0.5% (W:W) of the mass of the wet sludge, and then the mixture is stirred evenly with a stirrer at a stirring speed of 10 rpm / min.
Embodiment 3
[0039] The difference from Example 1 is:
[0040] The second layer of coating of the granules: the solid mixture formed by sterile solid cornstarch and L-lysine salt is added in proportion to the bacterium granules prepared in the first granulation, wherein the sterile solid cornstarch is added The amount is 3 times (W: W) of the mass of the wet sludge, and the amount of L-lysine added is 0.5% (W: W) of the mass of the wet sludge. Then, the mixture is stirred evenly with a stirrer, and the stirring speed is 10 rpm / min;
[0041] The second granulation of bacterial granules: Put the bacterium granules prepared in the previous step into the granulator, adjust the particle size to 50 mesh, and obtain 50 mesh lactic acid bacteria granules.
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