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Gradient illumination photobioreactor and application thereof

A technology of photobioreactor and reactor, which is applied in photobioreactor, specific-purpose bioreactor/fermenter, combination of bioreactor/fermenter, etc. Reduce the utilization rate of light energy and other problems to achieve the effect of improving conversion utilization rate and cell yield

Active Publication Date: 2015-03-25
PETROCHINA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, although higher light intensity distribution in the reactor can be obtained through reactor design, etc., the way of constant illuminance light source is mostly used, which makes the light intensity too high when the algae cell concentration is low in the early stage of cultivation. Decreased light utilization rate may even exceed the tolerance range of algae cells and affect normal proliferation
However, when the concentration is high, the light intensity cannot meet the requirements of microalgae growth, which limits the further increase of microalgae production and algae concentration.
At the same time, most of these reactors are fully mixed-flow reactors. When adopting a continuous and stable culture method, in order to obtain a high-concentration cell liquid, the reactor must also operate at a high cell concentration, thus requiring a high light intensity, resulting in Decrease in Light Energy Utilization Efficiency

Method used

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  • Gradient illumination photobioreactor and application thereof
  • Gradient illumination photobioreactor and application thereof
  • Gradient illumination photobioreactor and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] This embodiment shows the distribution of light quantum flux in the photoreactor of the present invention. The structural parameters of the reactor are as follows: the total height of the reactor is 70 cm, and the total length is 60 cm. The width w of one side of the compartment I is 10 cm, the height is 55 cm, the width of the light source chamber is 5 cm, the upper end of the light source chamber is 10 cm from the top of the compartment I, and the lower end of the light source chamber is 3 cm from the bottom of the compartment I. The width of compartment II is 10 cm, and the width of compartment III is 18 cm. The volume of liquid in the three compartments is: compartment I (two total) is 124L, compartment II (two total) is 58L, and compartment III is 59L. The overflow weir at the top of compartment I is 5cm high, the outlet of the overflow pipe is 20cm from the bottom of compartment II, and the overflow opening is 3cm wide. The gas outlet and the discharge port are a...

Embodiment 2

[0047] This embodiment shows the distribution of light quantum flux in the photoreactor of the present invention. The structural parameters of the reactor are as follows: the total height of the reactor is 110 cm, and the total length is 80 cm. The width w of one side of the compartment I is 12 cm, the height is 95 cm, the width of the light source chamber is 6 cm, the upper end of the light source chamber is 15 cm from the top of the compartment I, and the lower end of the light source chamber is 5 cm from the bottom of the compartment I. The width of compartment II is 10 cm, and the width of compartment III is 18 cm. The volume of liquid in the three compartments is: compartment I (two total) is 336L, compartment II (two total) is 146L, and compartment III is 146L. The height of the overflow weir is 10cm, the outlet of the overflow pipe is 35cm from the bottom of the compartment II, and the width of the overflow is 5cm. The gas outlet and the discharge port are arranged in ...

Embodiment 3

[0049] This example is the batch cultivation of marine chlorella sp using the photoreactor of the present invention. The reactor is the same as in Example 1. In the room, a fluorescent lamp is used to simulate sunlight, and the light parameters are maintained as in Example 1, and the light-dark cycle is 16h:8h. The medium is F / 2 modified medium, the inoculum concentration is 1.2×10 6 cell / mL. After inoculation, the algae were cultured in Compartment III for 48 hours and then transferred to Compartment II to continue the cultivation. At the same time, the new culture medium was refilled in Compartment III and kept pressing 1.2×10 6 Cell / mL inoculation; after 48 hours of culture in compartment II, transfer to compartment I to continue culturing, and at the same time transfer the next batch of algae liquid from compartment III to compartment II. Microalgae are cultured in compartment I for 112 hours and then collected Close. The ventilation volume in compartment III, compartment...

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Abstract

The invention relates to a gradient illumination photobioreactor and application thereof. The photobioreactor has a box-type structure, and is divided into a compartments I, compartments II and compartments III, which have light intensity from high to low; a pair of compartment I, a pair of compartment II, and a pair of compartment III are arranged in each reactor box body; liquid flows through an overflow pipe or an overflow port between the compartments; microalgae is cultured in 3 compartments with high intensity from high to low, and the microalgae liquid concentration and aeration amount are increased correspondingly according to the light intensity from low to high; and the photobioreactor with gradient light and the cultivation of microalgae can improve the conversion rate of light energy; and for the continuous culture of microalgae, the photobioreactor is equivalent to multiple complete-mixing-flow-reactors in series, and can improve the cell yield.

Description

Technical field [0001] The invention relates to a gradient-illuminated photobioreactor and a method for cultivating microalgae. Background technique [0002] Microalgae are important biological resources for obtaining food proteins, medicines and other materials, as well as the main raw materials and important technology platforms for the third generation of biofuels. The use of photobioreactors for large-scale cultivation of microalgae is an important way to solve the bottleneck of microalgae resource industrialization. The photobioreactor is a reactor that can provide a certain light intensity and hydrodynamic environment for illuminating biomass cultivation or photochemical reaction. Using photobiological reaction to cultivate microalgae can increase the culture concentration and proliferation rate of microalgae, save space, avoid the influence of external environment on microalgae culture, and continuously obtain microalgae resources with stable quality. [0003] The light in...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/04C12N1/12
CPCC12M21/02C12M23/34C12M29/06C12M31/10C12N1/12
Inventor 何皓蒋国强齐泮仑于常军孙洪磊王桂伟李建忠
Owner PETROCHINA CO LTD
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