Method for extracting chondroitin sulfate from fish cartilage
A technology for chondroitin sulfate and cartilage, applied in the field of bioengineering, can solve the problems of restricting the development of small molecule chondroitin sulfate, low yield of chondroitin sulfate, high price, etc., and achieves the advantages of reducing the amount of alcohol, short cycle and convenient operation. Effect
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Embodiment 1
[0021] (1) Primary screening of microorganisms: Suspend the soil sample with sterile water to make a 10% soil solution, filter through two layers of filter paper to obtain a clear liquid, and dilute the liquid gradient to 10% -5 , take 0.3ml of the diluted solution to spread and culture on the separation medium plate, and purify the single bacterial colony on the separation medium plate by the method of streaking the plate for 2 times. (NH 4 ) 2 SO 4 0.5%, K 3 PO 4 0.05%, NaCl 0.6% primary screening medium plate, control pH = 7.0, incubate in a 32°C incubator for 6-7 days, pour 6ml of 3mol / L glacial acetic acid into the plate, soak for 3min, will produce transparent The circled strains were stored on solid slant medium in test tubes by streaking method and stored at 4°C for later use;
[0022] (2) Microbial re-screening: Inoculate the bacteria that produced the transparent circle into the re-screening medium, culture at 32°C for 2 days with shaking and aeration at 135r / ...
Embodiment 2
[0029] (1) Primary screening of microorganisms: Suspend the soil sample with sterile water to make a 10% soil solution, filter through two layers of filter paper to obtain a clear liquid, and dilute the liquid gradient to 10% -5 , take 0.3ml of the diluted solution to spread and culture on the separation medium plate, and purify the single bacterial colony on the separation medium plate by the method of streaking the plate for 2 times. (NH 4 ) 2 SO 4 0.5%, K 3 PO 4 0.05%, NaCl 0.6% primary screening medium plate, control pH = 7.0, incubate in a 32°C incubator for 6-7 days, pour 6ml of 3mol / L glacial acetic acid into the plate, soak for 3min, will produce transparent The circled strains were stored on solid slant medium in test tubes by streaking method and stored at 4°C for later use;
[0030] (2) Microbial re-screening: Inoculate the bacteria that produced the transparent circle into the re-screening medium, culture at 32°C for 2 days with shaking and aeration at 135r / ...
Embodiment 3
[0037] (1) Primary screening of microorganisms: Suspend the soil sample with sterile water to make a 10% soil solution, filter through two layers of filter paper to obtain a clear liquid, and dilute the liquid gradient to 10% -5 , take 0.3ml of the diluted solution to spread and culture on the separation medium plate, and purify the single bacterial colony on the separation medium plate by the method of streaking the plate for 2 times. (NH 4 ) 2 SO 4 0.5%, K 3 PO 4 0.05%, NaCl 0.6% primary screening medium plate, control pH = 7.0, incubate in a 32°C incubator for 6-7 days, pour 6ml of 3mol / L glacial acetic acid into the plate, soak for 3min, will produce transparent The circled strains were stored on solid slant medium in test tubes by streaking method and stored at 4°C for later use;
[0038] (2) Microbial re-screening: Inoculate the bacteria that produced the transparent circle into the re-screening medium, culture at 32°C for 2 days with shaking and aeration at 135r / ...
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