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Method for enhancing Raman signals with nano super-crystal technology to identify microorganisms

A Raman signal and microorganism technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of long detection period, inaccurate detection, unable to meet the requirements of rapid and accurate identification of microorganisms, and achieve rapid identification and simple identification methods. , the effect of broad universality

Inactive Publication Date: 2015-03-25
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

[0002] At present, the commonly used microbial identification technology is mainly the plate counting method, which has a long detection period, usually three days to one week, and because its identification of the type of microorganisms can only be based on the shape of the microorganisms, the detection is not accurate enough to meet the needs of rapid microbial detection. Accurate Identification Requirements

Method used

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  • Method for enhancing Raman signals with nano super-crystal technology to identify microorganisms
  • Method for enhancing Raman signals with nano super-crystal technology to identify microorganisms
  • Method for enhancing Raman signals with nano super-crystal technology to identify microorganisms

Examples

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Embodiment 1

[0026] Example 1: Recognition of Escherichia coli by enhancing the intrinsic Raman signal of Escherichia coli through three-dimensional supercrystals of gold-silver core-shell nanorods

[0027] In this embodiment, the intrinsic Raman signal of Escherichia coli is enhanced by three-dimensional supercrystals of gold-silver core-shell nanorods to identify Escherichia coli, and the procedure is as follows:

[0028] Step 1: Dissolve 20 mL of 0.5 mM auric acid chloride (HAuCl 4 ) solution and 20mL, 0.2M cetyltrimethylammonium bromide (CTAB) were mixed under ice bath conditions to obtain a mixed solution; 1.2mL, 0.02M sodium borohydride (NaBH 4 ) into the mixed solution, under the condition of 1000rpm rotating speed, vigorously stir for 2 minutes, and stand at 25°C for 1 hour to obtain a solution containing gold nano-seeds;

[0029] Mix 200mL, 0.2M CTAB with 10mL, 4mM silver nitrate (AgNO 3 ) solutions were mixed at room temperature to form a yellow growth solution; 200mL, 1mM HAuC...

Embodiment 2

[0039] Example 2: Enhancing Raman signal to identify yeast by three-dimensional supercrystal of golden triangle nanosheet

[0040] In this embodiment, the Raman signal is enhanced by the three-dimensional supercrystal of the golden triangle nanosheet to identify the specific steps of the yeast:

[0041] Step 1: Dilute 1mL, 0.01M chloroauric acid solution and 1mL, 0.01M trisodium citrate solution with water to 40mL, mix under ice bath conditions to obtain a mixed solution, then add 1mL, 0.1M sodium borohydride, and rotate at 1000rpm Under the conditions, stir vigorously for 2 minutes, and stand at 25° C. for 2-4 hours to obtain a solution containing gold nanoparticles.

[0042] Then, 225mL, 0.05M CTAB, 1mL, 0.1M KI, 1.25mL, 0.1M ascorbic acid, 1.25mL, 0.1M NaOH, 6.25mL, 0.01M chloroauric acid solution were mixed to form a mixed growth solution. Then, take 22.5mL from Erlenmeyer flask C containing 225mL mixed growth solution into Erlenmeyer flask B, and take 2.25mL from Erlenme...

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Abstract

The invention discloses a method for enhancing Raman signals with a nano super-crystal technology to identify microorganisms. The method is characterized by comprising steps as follows: airing optically anisotropic precious metal nano-materials on silicon wafer substrates in a natural evaporation manner under humidity-controlled and temperature-controlled conditions so as to form direction-controllable and regularly-arranged three-dimensional super-crystals; enabling the to-be-tested microorganisms to be adsorbed on the three-dimensional super-crystals, amplifying the intrinsic Raman signals of the microorganisms by using a plasmon field of the three-dimensional super-crystals, and acquiring the intrinsic Raman signals of the to-be-tested microorganisms through detection with an enhanced Raman technology so as to identify the species of the to-be-tested microorganisms. According to the method, the nano super-crystals are used as the substrates, the microorganisms are adsorbed directly, the weak intrinsic Raman signals of the microorganisms are efficiently amplified, label-free quick identification of the microorganisms can be realized, and the long-cycle process for propagation and culture of the microorganisms is avoided.

Description

technical field [0001] The invention belongs to the field of microorganism detection, and specifically relates to optically anisotropic noble metal nano-supercrystals used as highly sensitive surface-enhanced Raman substrates to efficiently amplify the weak intrinsic Raman signals of microorganisms to quickly and effectively identify the presence and absence of microorganisms. type. Background technique [0002] At present, the commonly used microbial identification technology is mainly the plate counting method, which has a long detection period, usually three days to one week, and because its identification of the type of microorganisms can only be based on the shape of the microorganisms, the detection is not accurate enough to meet the needs of rapid microbial detection. Accurate identification requirements. Contents of the invention [0003] The present invention is to avoid the shortcomings of the above-mentioned prior art, and provides a method for enhancing Raman ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 王进贾皓玮邱丽
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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