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Rhodococcus sp. capable of degrading four herbicides and application thereof

A rhodococcus and herbicide technology, applied in the field of rhodococcus, can solve problems such as slow natural degradation

Active Publication Date: 2015-04-01
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The degradation of herbicide residues in nature mainly depends on microorganisms in the soil, but the natural degradation is very slow

Method used

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  • Rhodococcus sp. capable of degrading four herbicides and application thereof
  • Rhodococcus sp. capable of degrading four herbicides and application thereof
  • Rhodococcus sp. capable of degrading four herbicides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1. Isolation and identification of Rhodococcus sp. 198-R-56 CGMCC No.9868

[0053] 1. Isolation of herbicide-degrading bacteria 198-R-56

[0054] 1. Add 10g of soil samples (collected from farmland polluted by herbicides in Heihe, Heilongjiang, China) to 100ml of sterile water, shake and incubate for 30min to make a turbid solution. Take 1ml of the above cloudy solution and add it to a test tube filled with 9ml sterile water and mix well (at this time, the dilution is recorded as 10 -1 ), then draw 1ml from this test tube and add it to another test tube filled with 9ml sterile water and mix evenly, and so on to make 10 -2 、10 -3 、10 -4 、10 -5 Bacterial suspensions at different dilutions. Take 0.1ml of each dilution and evenly spread it on a nitrogen-free solid medium plate, and culture it at a constant temperature of 28°C for 2-3 days. After the colonies are formed, strains are purified on a nitrogen-free solid medium plate.

[0055] 2. The initial screen...

Embodiment 2

[0075] Example 2, Quantitative determination of the ability of Rhodococcus sp. 198-R-56 CGMCC No.9868 to degrade Pursit

[0076] 1. The drawing of the standard curve for Pushett determination

[0077] Accurately weigh 10.0 mg of Pushte standard substance (product of Fluka Company) into a 10 mL volumetric flask, dissolve it with a small amount of methanol, place the volumetric flask in an ultrasonic bath and vibrate for 10 min, then dilute to 10 mL with methanol, shake well, and prepare 1000mg / L Pushite mother liquor. Continue to dilute with methanol to prepare 20, 40, 60, 80, 100mg / L series of Pushite standard solution. High-performance liquid chromatography (HPLC) was used to measure the peak areas of different concentrations of Pushte standards, and repeated 3 times. Take the Pusite concentration as the abscissa, and the peak area as the ordinate, draw the Pusite standard curve, such as figure 1 shown.

[0078] The detection conditions are as follows:

[0079] Detectio...

Embodiment 3

[0088] Example 3, Quantitative Determination of Acetochlor Degradation Ability of Rhodococcus sp. 198-R-56 CGMCC No.9868

[0089] 1. Preparation of standard curve for acetochlor determination

[0090] Accurately weigh 10.0 mg of acetochlor standard product (Fluka company product) in a 10 mL volumetric flask, dissolve it with a small amount of methanol, place the volumetric flask in an ultrasonic bath and vibrate for 10 min, then dilute to 10 mL with methanol, shake well, and prepare 1000mg / L acetochlor mother liquor. Then dilute with methanol to obtain concentration and be respectively 10, 20, 40, 60, 80, 100mg / L standard solution, adopt high performance liquid chromatography (HPLC) to measure the peak area of ​​different concentration acetochlor standard substance, repeat 3 times. Taking the concentration of acetochlor as the abscissa, and the peak area as the ordinate, draw the acetochlor standard curve, such as figure 2 shown.

[0091] The detection conditions are as fo...

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Abstract

The invention discloses a bacterium capable of degrading herbicides and an application thereof. The bacterium capable of degrading herbicides is rhodococcus sp. 198-R-56, and the collection number of the bacterium in the China General Microbiological Culture Collection Centre is CGMCC No. 9868. With regard to the rhodococcus sp. 198-R-56 CGMCC No. 9868 provided by the invention, in an inorganic salt culture medium for 7 days, the degradation rate for 100mg / L of imazethapyr achieves 84.63%, the degradation rate for 50mg / L of acetochlor achieves 69.15%, the degradation rate for 100mg / L of chlorimuron-ethyl achieves 52.20%, and the degradation rate for 100mg / L of acifluorfen sodium achieves 20.16%, which indicates that the strain is capable of degrading the herbicides imazethapyr, acetochlor, chlorimuron-ethyl and acifluorfen sodium; meanwhile, the strain has a capacity of producing plant hormone heteroauxin and has a yield of 62.35+ / -3.76mu g / ml, which indicates that the strain has a capacity of promoting plant growth.

Description

technical field [0001] The invention relates to a bacterium capable of degrading herbicides and its application in the field of microorganisms, in particular to a rhodococcus strain degrading four herbicides and its application. Background technique [0002] Pursuit, also known as imazethapyr, English name Pursuit (imazethapyr-ammonium), chemical name (RS)-5-ethyl-2-(4-isopropyl-4-methyl-5-oxo-2 -imidazolin-2-yl) nicotinic acid. Pushit is an imidazolinone herbicide and an inhibitor of side chain amino acid synthesis. It can be applied before or after emergence. It is effective against grass weeds and certain broad-leaved weeds such as amaranth in soybeans and other legume farmlands. , Polygonum, Chenopodium, Solanum nigrum, Cocklebur, Barnyardgrass, Setaria, crabgrass, millet, etc. have excellent control effects. [0003] Acetochlor, English common name acetochlor, chemical name is 2-ethyl-6 methyl-N-ethoxymethyl-α-chloroacetanilide. Acetochlor is a systemic amide herbici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/10A62D3/02A01N63/00A01P21/00B09C1/10C12R1/01A62D101/04A62D101/28
CPCA01N63/00A62D3/02A62D2101/04B09C1/10C12P17/10C12N1/205C12R2001/01
Inventor 高淼孙建光孙颖飞杨慧
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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