Camellia nitidissima sprout tissue culture and rapid propagation method

A technology for tissue culture, rapid propagation, and golden camellia, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as species that are not seen with golden colors, and is conducive to industrial production and automatic monitoring, reducing electricity. Consumption, increase the effect of multiplying multiples

Inactive Publication Date: 2015-04-08
郓城县华昌食品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previously, people had not se

Method used

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Embodiment 1

[0027] A method for tissue culture and rapid propagation of young bud golden camellia, comprising the steps of:

[0028](1) Disinfection of explants: at 1 o'clock in the evening in June, pick golden camellia tea leaves that are light green, complete, and free from diseases and insect pests, and then use 20% dimethoate emulsion to dilute 400 times and soak for 5 minutes. After taking them out, put them into an ultra-clean workbench Soak in 4% vitamin C aqueous solution for 30 minutes, then disinfect with 3% hypochlorous acid for 5 minutes, then rinse with sterile water for 3 times, and finally use sterile filter paper to dry the sterile water on the surface of the golden camellia tea;

[0029] (2) Callus culture: Use a scalpel to cross-cut the leaf strips with a length of 15 cm and a width of 10 cm along the stem of the golden camellia tea, and put 3 leaf strips into the callus culture medium In a petri dish with a specification of 90×15mm, lightly press the leaf strips, cultur...

Embodiment 2

[0034] A method for tissue culture and rapid propagation of young bud golden camellia, comprising the steps of:

[0035] (1) Disinfection of explants: at 2:00 p.m. in July, pick golden camellia tea leaves that are light green, complete, and free from diseases and insect pests, and then soak them for 10 minutes with 40% dimethoate emulsion diluted 600 times, take them out and put them into an ultra-clean workbench Soak in 4% vitamin C aqueous solution for 30 minutes, then disinfect with 3% hypochlorous acid for 10 minutes, then rinse with sterile water for 5 times, and finally use sterile filter paper to dry the sterile water on the surface of the golden camellia tea;

[0036] (2) Callus culture: use a scalpel to cross-cut the leaf strips with a length of 18cm and a width of 15cm along the stem of the golden camellia tea, and put 5 pieces of leaf strips into the callus culture medium In a petri dish with a specification of 90×15mm, lightly press the leaf strips, culture in the ...

Embodiment 3

[0041] A method for tissue culture and rapid propagation of young bud golden camellia, comprising the steps of:

[0042] (1) Disinfection of explants: at 1:20 p.m. in June, pick golden camellia tea leaves that are light green in color, complete, and free from diseases and insect pests, and then soak them in 450 times liquid diluted with 25% dimethoate emulsion for 6 minutes. Soak the clean workbench in 4% vitamin C aqueous solution for 30 minutes, then disinfect it with 3% hypochlorous acid for 6 minutes, then rinse it with sterile water for 4 times, and finally use sterile filter paper to dry the sterile water on the surface of the golden camellia tea;

[0043] (2) Callus culture: Use a scalpel to cross-cut leaves 16cm in length and 11cm in width along the stem of Camellia japonica, and put 4 leaves into the callus culture medium In a petri dish with a specification of 90×15mm, lightly press the leaf strips, culture in the dark at 30°C for 9 days, and then transfer to 27°C fo...

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Abstract

The invention discloses a camellia nitidissima sprout tissue culture and rapid propagation method. The camellia nitidissima sprout tissue culture and rapid propagation method is characterized by comprising the following steps: (1) disinfecting explants; (2) culturing calluses; (3) differentiating shoots from the calluses; and (4) rooting the shoots. The camellia nitidissima sprout tissue culture and rapid propagation method disclosed by the invention has the advantages of propagation cost reduction, short growth cycle, high propagation rate and convenience of management, is suitable for industrial production and automatic monitoring and is beneficial to saving manpower, material resources and crop field greatly.

Description

technical field [0001] The invention belongs to the technical field of tissue culture production, and relates to a method for tissue culture and rapid propagation of young bud camellia camellia. Background technique [0002] Camellia Camellia belongs to the family Theaceae and Camellia genus, and it is twin sisters with Tea, Camellia, Camellia oleifera, Camellia oleifera, and Camellia sasana. The flowers of Camellia japonica are golden yellow, dazzling, as if coated with a layer of wax, crystal clear and oily, as if translucent. Camellia chrysalis grows solitary in the leaf axils. When the flowers bloom, there are cup-shaped, pot-shaped or bowl-shaped flowers, which are delicate and colorful, beautiful and elegant. Previously, people had not seen species with golden flowers. In 1960, Chinese scientists first discovered a golden yellow camellia in Nanning, Guangxi, which was named Camellia Camellia. The oriental magic tea, which is called miraculous abroad, is known as "th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张莘蔓韦玲菊陈程罗生芳
Owner 郓城县华昌食品有限公司
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