Method for reducing heavy metal cadmium in rice by utilizing mixed fermentation of lactobacillus and saccharomycetes
A technology of mixed fermentation and yeast, which is applied in the fields of application, food preparation, food science, etc., can solve the problems of reducing the protein and ash content of rice flour, achieve high removal rate, broad market application prospects, and significant social and economic benefits Effect
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[0016] Preparation of the Lactobacillus plantarum seed culture solution: Take the Lactobacillus plantarum solution and insert it into the sterilized MRS broth medium under aseptic conditions, the inoculum size is 4%, cultivate it at a constant temperature at 37°C for 28 hours, and dilute it until the concentration reaches 7×10 8 cfu / ml.
[0017] Preparation of Saccharomyces cerevisiae seed culture medium: Scrape Saccharomyces cerevisiae slant bacteria with an inoculation loop under sterile conditions and inoculate them into YPD medium, culture at 30°C for 28 hours, and dilute to a concentration of 6×10 7 cfu / ml.
[0018] Preparation of Lactobacillus acidophilus seed culture medium: Add sterilized MRS broth medium to Lactobacillus acidophilus lyophilized powder according to the ratio of 5ml Lactobacillus acidophilus lyophilized powder plus 1ml sterilized MRS broth medium , shake to dissolve all the lyophilized powder of Lactobacillus acidophilus into a suspension, put it in...
Embodiment 1
[0020] Take Lactobacillus plantarum culture solution, Saccharomyces cerevisiae culture solution and Lactobacillus acidophilus culture solution, and mix them according to the volume ratio of 7:6:7 to make a mixed fermentation seed solution; take rice with a heavy metal cadmium content of 0.4mg / kg, and grind it into powder , through a 60-mesh sieve, according to the ratio of 1g rice flour to 4ml deionized water, add deionized water to the rice flour, mix well, and make rice slurry; add 8% (w / w) mixed fermented seeds to the rice slurry fermented rice flour; wash the fermented rice flour with deionized water 3 times, and the amount of deionized water each time is 5 times the weight of the fermented rice flour , the final cleaning was performed by centrifugation (3000rpm, 10min) to remove deionized water, and the filter residue (fermented rice flour) was dried with hot air at 45°C until the water content was 13.5%, to obtain rice flour with reduced heavy metal cadmium. After testin...
Embodiment 2
[0030] Take Lactobacillus plantarum seed culture solution, Saccharomyces cerevisiae seed culture solution, and Lactobacillus acidophilus seed culture solution, and mix them at a volume ratio of 7:6:7 to make a mixed fermentation seed solution; take rice with a heavy metal cadmium content of 0.4mg / kg Grind into powder, pass through a 40-mesh sieve, add deionized water to the rice flour according to the ratio of 1g rice flour to 6ml deionized water, mix well, and make rice slurry; add 7% (w / w) to the rice slurry Mix the fermented seed liquid, leave it to ferment at 37°C for 28 hours, filter and remove the fermented liquid to obtain fermented rice flour; wash the fermented rice flour with deionized water 4 times, and the amount of deionized water used each time is the weight of the fermented rice flour The final washing was performed by centrifugation (5000rpm, 5min) to remove deionized water, and the filter residue (fermented rice flour) was dried with hot air at 50°C until the m...
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