Method for reducing heavy metal cadmium in rice by utilizing mixed fermentation of lactobacillus and saccharomycetes

A technology of mixed fermentation and yeast, which is applied in the fields of application, food preparation, food science, etc., can solve the problems of reducing the protein and ash content of rice flour, achieve high removal rate, broad market application prospects, and significant social and economic benefits Effect

Active Publication Date: 2015-04-08
HUNAN AGRICULTURAL UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Wang Feng (2003) studied the components of rice flour after soaking and fermentation, and found that the protein and ash content in rice flour were significantly r

Method used

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Examples

Experimental program
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preparation example Construction

[0016] Preparation of the Lactobacillus plantarum seed culture solution: Take the Lactobacillus plantarum solution and insert it into the sterilized MRS broth medium under aseptic conditions, the inoculum size is 4%, cultivate it at a constant temperature at 37°C for 28 hours, and dilute it until the concentration reaches 7×10 8 cfu / ml.

[0017] Preparation of Saccharomyces cerevisiae seed culture medium: Scrape Saccharomyces cerevisiae slant bacteria with an inoculation loop under sterile conditions and inoculate them into YPD medium, culture at 30°C for 28 hours, and dilute to a concentration of 6×10 7 cfu / ml.

[0018] Preparation of Lactobacillus acidophilus seed culture medium: Add sterilized MRS broth medium to Lactobacillus acidophilus lyophilized powder according to the ratio of 5ml Lactobacillus acidophilus lyophilized powder plus 1ml sterilized MRS broth medium , shake to dissolve all the lyophilized powder of Lactobacillus acidophilus into a suspension, put it in...

Embodiment 1

[0020] Take Lactobacillus plantarum culture solution, Saccharomyces cerevisiae culture solution and Lactobacillus acidophilus culture solution, and mix them according to the volume ratio of 7:6:7 to make a mixed fermentation seed solution; take rice with a heavy metal cadmium content of 0.4mg / kg, and grind it into powder , through a 60-mesh sieve, according to the ratio of 1g rice flour to 4ml deionized water, add deionized water to the rice flour, mix well, and make rice slurry; add 8% (w / w) mixed fermented seeds to the rice slurry fermented rice flour; wash the fermented rice flour with deionized water 3 times, and the amount of deionized water each time is 5 times the weight of the fermented rice flour , the final cleaning was performed by centrifugation (3000rpm, 10min) to remove deionized water, and the filter residue (fermented rice flour) was dried with hot air at 45°C until the water content was 13.5%, to obtain rice flour with reduced heavy metal cadmium. After testin...

Embodiment 2

[0030] Take Lactobacillus plantarum seed culture solution, Saccharomyces cerevisiae seed culture solution, and Lactobacillus acidophilus seed culture solution, and mix them at a volume ratio of 7:6:7 to make a mixed fermentation seed solution; take rice with a heavy metal cadmium content of 0.4mg / kg Grind into powder, pass through a 40-mesh sieve, add deionized water to the rice flour according to the ratio of 1g rice flour to 6ml deionized water, mix well, and make rice slurry; add 7% (w / w) to the rice slurry Mix the fermented seed liquid, leave it to ferment at 37°C for 28 hours, filter and remove the fermented liquid to obtain fermented rice flour; wash the fermented rice flour with deionized water 4 times, and the amount of deionized water used each time is the weight of the fermented rice flour The final washing was performed by centrifugation (5000rpm, 5min) to remove deionized water, and the filter residue (fermented rice flour) was dried with hot air at 50°C until the m...

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Abstract

The invention discloses a method for reducing heavy metal cadmium in rice by utilizing mixed fermentation of lactobacillus and saccharomycetes. The method comprises the following steps of crushing and sieving a rice sample with the cadmium content exceeding the standard (0.2mg/kg above), adding deionized water, charging mixed fermentation bacterium suspension of lactobacillus plantarum, brewer's yeast and lactobacillus acidophilus, stewing and fermenting at constant temperature, washing and centrifugally dehydrating the fermented rice flour, removing the fractionated heavy metal cadmium ion in rice, then drying the centrifuged wet rice flour residues so as to obtain the rice flour with reduced heavy metal cadmium. By adopting the method, the reduction rate of heavy metal cadmium in the rice sample reaches 85% above; drying the dehydrated rice flour for acting as a feed or for producing fermented rice flour. According to the invention, not only can the content of heavy metal cadmium in rice be reduced effectively, but also the utilization issue of rice with cadmium exceeding the standard is solved effectively.

Description

technical field [0001] The invention belongs to the technical field of rice deep processing, and in particular relates to a method for reducing heavy metal cadmium in rice by mixed fermentation of lactobacillus and yeast. Background technique [0002] In recent years, due to the development of industry, the soil for planting rice has been polluted, resulting in serious excess of heavy metal cadmium in rice. Zhang Liangyun (2009) found that the cadmium content of more than 30% of rice in southern my country exceeded the national food safety standard value of 0.2 mg / kg. The current situation of excessive cadmium in rice and rice products needs to be improved urgently. However, in recent years, relevant research at home and abroad: methods such as rice breeding and soil improvement have not achieved significant results and should be promoted. Liu Jing (2013) discovered the changing law of heavy metal content in the process of soaking rice; some scholars have also proved throu...

Claims

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Application Information

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IPC IPC(8): A23L1/105A23L1/015A23L7/104A23L5/20
Inventor 吴卫国卢露廖卢艳王巨涛
Owner HUNAN AGRICULTURAL UNIV
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