Method for establishing domestic rabbit atherosclerosis model

An atherosclerosis and rabbit technology, applied in the field of experimental animal disease models, can solve the problems of long time, unfavorable promotion and use, high price, etc., and achieve the effect of reducing the cost of consumables and saving animal feeding and management costs.

Inactive Publication Date: 2015-04-08
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of "high-fat feed + intravenous bovine serum albumin + arterial endothelial balloon strain" takes 12 weeks, which takes a long time, and the consumables "arterial balloon" and "pressure pump" used are expensive and unreasonable. Good for promotion

Method used

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  • Method for establishing domestic rabbit atherosclerosis model
  • Method for establishing domestic rabbit atherosclerosis model
  • Method for establishing domestic rabbit atherosclerosis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. After the male Japanese big-eared white rabbits were weighed and numbered, except for the male Japanese big-eared white rabbits in the control group, the male Japanese big-eared white rabbits in the other experimental groups were tested on the first day and the seventh day of modeling. Big-eared white rabbits were injected with bovine serum albumin from the ear vein, and the dose of each injection was 250mg / kg. Immediately after the injection on the first day, the left femoral artery strain was performed under general anesthesia, and high-fat feed was fed on the same day until the seventh week.

[0029] The operation method of the left femoral artery strain operation: the male Japanese white rabbits in the experimental group fasted for 12 hours before the operation but did not need to be deprived of water; after anesthesia with 3% sodium pentobarbital (30mg / kg), the animals were fixed in a On the operating table; the skin around the left groin was clipped for routine...

Embodiment 2

[0034] After the puncture was successful, the radial artery sheath was inserted to a depth of 5 cm by means of a guide wire. The 2.0 kg rabbit chose to use a 5F sheath, and then injected heparin 200 U / Kg into the ear vein, and the others were the same as in Example 1.

Embodiment 3

[0036] After the puncture was successful, the radial artery sheath was inserted to a depth of 5 cm by means of a guide wire. The 2.2 kg rabbit chose to use a 6F sheath, and then injected heparin 200 U / Kg into the ear vein, and the others were the same as in Example 1.

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Abstract

The invention discloses a method for establishing a domestic rabbit atherosclerosis model, wherein the method includes high-lipidation processing on a domestic rabbit basic feed and intravenous injection of bovine serum albumin, also includes combined use of a vagina vasorum and a forcemeter for making domestic rabbit artery blood vessel endothelium injured, and particularly includes the following steps: carrying out paracentesis on a middle-lower segment of a femoral artery by 21G radial artery puncture needles; after the paracentesis is successful, inserting into a radial artery sheathing canal with the depth of 5 cm with the help of a guide wire; followed by, carrying out intravenous injection of 200 U/kg of heparin at an ear edge; after pulling out the guide wire, fixing a fine line at the tail end of the vagina vasorum, making the other end of the fine line connected with the forcemeter, allowing fingers of a left hand of an operative doctor to draw close to each other and press a femoral artery part internally containing the vagina vasorum, allowing a right hand to hold the forcemeter to pull out the vagina vasorum to 4 cm, and allowing the left hand pressing force to be 10 N displayed in the forcemeter as a degree, so as to cause vascular endothelial injury. The invention aims to provide the modeling method for the domestic rabbit atherosclerosis model, wherein the modeling method is short in period, strong in operability and relatively low in cost.

Description

technical field [0001] The invention belongs to the category of experimental animal disease models, in particular to a method for establishing a rabbit atherosclerosis model. Background technique [0002] Atherosclerosis is a chronic reactive inflammatory disease involving the immune system. It is the main pathological basis of cardiovascular and cerebrovascular diseases, seriously endangers human health, and is the main cause of cardiovascular and cerebrovascular diseases. However, its pathogenesis has not yet been fully elucidated, and in-depth experimental research is needed. Therefore, there is an urgent need for a fast, economical, easy-to-manufacture and replicate animal model that is closer to the pathogenesis of human atherosclerosis. [0003] Zhang Junping and others once proposed in the report of "Chinese Journal of Comparative Medicine" Volume 19, Issue 1, 2009 that "high-fat feed + intravenous bovine serum albumin + arterial endothelial balloon strain" method was...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/513A61K38/38A01K67/027A61D1/00
CPCA01K67/027A61D1/00A61K31/513A61K38/385
Inventor 乔寅飞朱克俭肖长江柏正平李勇敏肖梅英翦林宏喻正科韩育明赵启李志颜旭谭宇军张婷朱筱婧杨文丽夏相宜
Owner HUNAN UNIV OF CHINESE MEDICINE
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