Ultramicro droplets manipulator based on liquid residues and method thereof

Abstract

The invention discloses an ultramicro droplets manipulator based on liquid residues. which comprises a porous plate, an ultramicro droplets array chip; a liquid collecting probe comprising a support part and a liquid collecting end at bottom of the support part; wherein the surface of the liquid collecting end has endophilicity to liquid to be collected, and the external surface of the support part has no endophilicity to liquid to be collected; and a switching mechanism; so that the sampling process of the liquid collecting probe on the liquid to be collected as well as sample placing processes that the liquid to be collected is placed in the ultramicro droplets array chip can be carried out in order. The invention also discloses a method for control the ultramicro droplets based on liquid residues. According to the invention, volume precision with lower than PL grade and even low to FL grade to AL grade can be reached, consumption of samples and reagents in chemical and biology reaction and analysis can be effectively reduced, experiment cost is saved, driving parts such as an injection pump and a peristaltic pump as well as pipelines such as capillary are not required, system operation is simple, reliability is high, array form and automation can be easily realized.

Description

technical field [0001] The invention belongs to the field of microfluidic droplet analysis in analytical chemistry, and in particular relates to an ultra-micro droplet control device and method based on liquid residue. Background technique [0002] Micro-volume liquid manipulation methods and devices are playing an increasingly important role in the current developments in the field of analytical chemistry. On the one hand, the reduction in the volume of liquid handling reduces the demand for rare samples, significantly reducing the difficulty and cost of experiments. On the other hand, the volume effect of the microsystem avoids excessive dilution of ultra-micro samples, thus realizing chemical analysis tasks that are difficult for conventional large-volume systems, such as single-cell and single-molecule analysis. Microfluidic chip technology uses micro-electromechanical processing technology to process networked micron-scale channels on substrates such as glass or plasti...

AI Technical Summary

Problems solved by technology

However, due to the use of liquid measuring methods based on pressure-driven technologies such as syringe pumps and peristaltic pumps, affected by mechanical motion accuracy and interfacial tension in capillary channels, the liquid manipulation volume of the system is usually tens of picoliters to nanoliters, which is difficult Precise control of ultra-micro liquid volumes below picoliters

Especially for multi-sample systems that can manipulate ultra-micro-volume liquids at a level below the picoliter level, there is no literature report yet

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