Primer pair and kit for identification or assisted identification of stored bean weevils

Abstract

The invention discloses a primer pair and a kit for identification or assisted identification of stored bean weevils. The primer pair disclosed by the invention is composed of the following five primer pairs: a primer pair 1 (a sequence 1 and a sequence 2), a primer pair 2 (a sequence 3 and a sequence 4), a primer pair 3 (a sequence 5 and a sequence 6), a primer pair 4 (a sequence 7 and a sequence 8) and a primer pair 5 (a sequence 9 and a sequence 10). Used for identification of species of the stored bean, the kit is not affected by the individual development status of a sample and the integrity of the sample, and has the advantages of being time-saving, high-efficient and accurate; and the primer pair and the kit can be widely applied to the food storage departments and quarantine departments.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a pair of primers for identifying or assisting in the identification of stored bean elephants and a kit thereof. Background technique

[0002] Bean weevils belong to Coleoptera (Coleoptera) Bean weevil family (Bruchinae), distributed in all continents of the world except Antarctica, especially in the tropical regions of Asia, Central America and South America. There are currently about 1,400 species of bean weevils recorded in the world, belonging to 58 genera. About 84% of the species of insects in this family feed on the seeds of legumes, becoming an important class of pests of cultivated legumes. There are 18 species of bean weevils recorded in my country's stored products, among which mung bean weevil Callosobruchus chinensis (Linnaeus), broad bean weevil Bruchus rufimanus Boheman and pea weevil Bruchus pisorum (Linnaeus) are the main stored bean pests in my country; and kidney ...

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