Tetracycline fermentation feeding medium and feeding method

A technology of feeding medium and tetracycline, which is applied in the field of tetracycline fermentation feeding medium and feeding, can solve the problems of fermentation broth turning sour to red, lack of dissolved oxygen, hindered synthesis of secondary metabolites and the like

Active Publication Date: 2015-04-15
NINGXIA QIYUAN PHARMA
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, during the fermentation process of Streptomyces aureus as the starting strain, mixed sugar water containing a large amount of carbon and nitrogen sources and phosphorus elements is added throughout the whole process, and there will be problems in the middle and late sta

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of slant spores: Inoculate Streptomyces aureus on a solid medium under aseptic conditions, culture at a constant temperature of 35°C for 4-5 days, and then inoculate in the seed medium.

[0024] 2. Seed culture: Inoculate the slant spores grown in step 1 into the seed medium in an aseptic manner, the inoculum amount is 15% of the volume in a 500ml eggplant-shaped bottle, culture at a constant temperature of 32°C, and the aeration ratio is 0.6-0.8v / v / min, the stirring speed is 400-600r / min, and cultivated for 20-26 hours.

[0025] 3. Fermentation culture: Inoculate the seeds cultivated in step 2 into the fermentation medium under aseptic conditions, the inoculum size is 10-20%, control the culture temperature 0-40hr: 33°C, 41-80hr: 32°C, 80hr- put The tank is at 31°C, the process ventilation ratio is 0.8-1.5v / min, the dissolved oxygen in the process is guaranteed not to be lower than 20%, and the culture is 6-8 days.

[0026] 4. Preparation of sugar wate...

experiment example 2

[0039] 1. Preparation of slant spores: Inoculate Streptomyces aureus on a solid medium under aseptic conditions, culture at a constant temperature of 35° C. for 4-5 days, and inoculate in the seed medium.

[0040] 2. Seed culture: Inoculate the slant spores grown in step 1 into the seed medium in an aseptic manner, the inoculum amount is 15% of the volume in a 500ml eggplant-shaped bottle, culture at a constant temperature of 32°C, and the aeration ratio is 0.6-0.8v / v / min, the stirring speed is 400-600r / min, and cultivated for 20-26 hours.

[0041] 3. Fermentation culture: Inoculate the seeds cultivated in step 2 into the fermentation medium under aseptic conditions, the inoculum size is 10-20%, control the culture temperature 0-40hr: 32°C, 41-80hr: 31°C, 80hr- put The tank is 30°C, the process ventilation ratio is 0.8-1.5v / min, and the dissolved oxygen in the process is guaranteed to be no less than 20%, and the culture is 6-8 days.

[0042] 4. Preparation of sugar water: ...

experiment example 3

[0055] 1. Preparation of slant spores: Inoculate Streptomyces aureus on a solid medium under aseptic conditions, culture at a constant temperature of 35° C. for 4-5 days, and inoculate in the seed medium.

[0056] 2. Seed culture: Inoculate the slant spores grown in step 1 into the seed medium in an aseptic manner, the inoculum amount is 15% of the volume in a 500ml eggplant-shaped bottle, culture at a constant temperature of 32°C, and the aeration ratio is 0.6-0.8v / v / min, the stirring speed is 400-600r / min, and cultivated for 20-26 hours.

[0057] 3. Fermentation culture: Inoculate the seeds cultivated in step 2 into the fermentation medium under aseptic conditions, the inoculum size is 10-20%, control the culture temperature 0-40hr: 32°C, 41-80hr: 31°C, 80hr- put The tank is 30°C, the process ventilation ratio is 0.8-1.5v / min, and the dissolved oxygen in the process is guaranteed to be no less than 20%, and the culture is 6-8 days.

[0058] 4. Preparation of sugar water: ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a tetracycline fermentation feeding medium and a feeding method. According to different demands of thalli for available nitrogen sources and phosphorus amount at different growth and metabolism stages, the amount of available nitrogen sources and phosphorus in the sugar water fed to thalli at different growth stages is improved so as to reach the purpose of improving the tetracycline fermentation level. Experiments prove that, feeding of low-phosphorous sugar water when fermentation is carried out to 60-100h can effectively control the primary metabolism rate, reduce the dissolved oxygen demand, and at the same time is beneficial to formation of secondary metabolites, and the tetracycline yield is significantly increased. The method provided by the invention is simple and practicable, and has reference significance to fermentation controlled by similar ways.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a tetracycline fermentation feeding medium and feeding method. Background technique [0002] Tetracycline is an antibacterial substance produced by fermentation of Streptomyces aureus. Tetracycline has a good inhibitory effect on Gram-positive bacteria, negative bacteria, Rickettsia, viral viruses, spirochetes and protozoa, and is a spectrum antibiotic. [0003] In the prior art, during the fermentation process of Streptomyces aureus as the starting strain, mixed sugar water containing a large amount of carbon and nitrogen sources and phosphorus elements is added throughout the whole process, and there will be problems in the middle and late stages due to excessive phosphorus content, excessive primary metabolism, and secondary The synthesis of metabolites is hindered. At the same time, due to the rapid primary metabolism and the excessive mycelium, the lack of dissol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P29/00C12R1/49
Inventor 章慧霞李学兵孙瑞君罗少奋张志
Owner NINGXIA QIYUAN PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap