Tetracycline fermentation feeding medium and feeding method
A technology of feeding medium and tetracycline, which is applied in the field of tetracycline fermentation feeding medium and feeding, can solve the problems of fermentation broth turning sour to red, lack of dissolved oxygen, hindered synthesis of secondary metabolites and the like
Examples
Embodiment 1
[0023] 1. Preparation of slant spores: Inoculate Streptomyces aureus on a solid medium under aseptic conditions, culture at a constant temperature of 35°C for 4-5 days, and then inoculate in the seed medium.
[0024] 2. Seed culture: Inoculate the slant spores grown in step 1 into the seed medium in an aseptic manner, the inoculum amount is 15% of the volume in a 500ml eggplant-shaped bottle, culture at a constant temperature of 32°C, and the aeration ratio is 0.6-0.8v / v / min, the stirring speed is 400-600r / min, and cultivated for 20-26 hours.
[0025] 3. Fermentation culture: Inoculate the seeds cultivated in step 2 into the fermentation medium under aseptic conditions, the inoculum size is 10-20%, control the culture temperature 0-40hr: 33°C, 41-80hr: 32°C, 80hr- put The tank is at 31°C, the process ventilation ratio is 0.8-1.5v / min, the dissolved oxygen in the process is guaranteed not to be lower than 20%, and the culture is 6-8 days.
[0026] 4. Preparation of sugar wate...
experiment example 2
[0039] 1. Preparation of slant spores: Inoculate Streptomyces aureus on a solid medium under aseptic conditions, culture at a constant temperature of 35° C. for 4-5 days, and inoculate in the seed medium.
[0040] 2. Seed culture: Inoculate the slant spores grown in step 1 into the seed medium in an aseptic manner, the inoculum amount is 15% of the volume in a 500ml eggplant-shaped bottle, culture at a constant temperature of 32°C, and the aeration ratio is 0.6-0.8v / v / min, the stirring speed is 400-600r / min, and cultivated for 20-26 hours.
[0041] 3. Fermentation culture: Inoculate the seeds cultivated in step 2 into the fermentation medium under aseptic conditions, the inoculum size is 10-20%, control the culture temperature 0-40hr: 32°C, 41-80hr: 31°C, 80hr- put The tank is 30°C, the process ventilation ratio is 0.8-1.5v / min, and the dissolved oxygen in the process is guaranteed to be no less than 20%, and the culture is 6-8 days.
[0042] 4. Preparation of sugar water: ...
experiment example 3
[0055] 1. Preparation of slant spores: Inoculate Streptomyces aureus on a solid medium under aseptic conditions, culture at a constant temperature of 35° C. for 4-5 days, and inoculate in the seed medium.
[0056] 2. Seed culture: Inoculate the slant spores grown in step 1 into the seed medium in an aseptic manner, the inoculum amount is 15% of the volume in a 500ml eggplant-shaped bottle, culture at a constant temperature of 32°C, and the aeration ratio is 0.6-0.8v / v / min, the stirring speed is 400-600r / min, and cultivated for 20-26 hours.
[0057] 3. Fermentation culture: Inoculate the seeds cultivated in step 2 into the fermentation medium under aseptic conditions, the inoculum size is 10-20%, control the culture temperature 0-40hr: 32°C, 41-80hr: 31°C, 80hr- put The tank is 30°C, the process ventilation ratio is 0.8-1.5v / min, and the dissolved oxygen in the process is guaranteed to be no less than 20%, and the culture is 6-8 days.
[0058] 4. Preparation of sugar water: ...
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Description
Claims
Application Information
- IPC
- C12P29/00; C12R1/49
- Inventors
- 章慧霞; 李学兵