A kind of genetically engineered bacteria expressing membrane-penetrating peptide fusion protein and its application

A technology of genetically engineered bacteria and fusion proteins, applied to genetically engineered bacteria expressing penetrating peptide fusion proteins and application fields, can solve the problems of asynchrony of virus speed, increase of virus titer, decrease of virus activity, etc. The effect of simple production and operation process and low cost
CN104530241BInactive Publication Date: 2018-05-18武汉市畜牧兽医科学研究所
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
武汉市畜牧兽医科学研究所
Publication Date
2018-05-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a genetically engineered bacterium expressing a membrane-penetrating peptide fusion protein and its application. The present invention provides a genetically engineered bacterium, E. coli BL21 / pET‑32a-CSFR, CCTCC NO: M2014631. The strain can express protein transduction domain polypeptide (PEP‑1) and classical swine fever virus receptor (CD46) genetically engineered fusion protein PEP‑1‑CD46. Its advantages are large expression, soluble protein, low cost and good safety. The expressed PEP‑1‑CD46 protein can be used as a mediating protein for CSFV to cross the cell membrane, improve the virus titer of CSFV in cell culture, and solve the problem that the current CSFV cell vaccine has a low virus content and cannot meet the vaccine standard Shortcomings.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, in particular to a genetically engineered bacterium expressing a penetrating peptide fusion protein and its application. technical background

[0002] Swine fever (classical swine fever, CSF) is an acute, febrile and lethal infectious disease of pigs caused by classical swine fever virus (CSFV). The disease is highly contagious and once widespread internationally, with a high morbidity rate and a mortality rate of over 90%, which is extremely harmful and caused huge economic losses to the pig industry at that time. Included in the list of important livestock and poultry infectious diseases. At present, my country is the country with the largest production and use of swine fever vaccines in the world. The total annual consumption is about 4 billion copies, and the total market price is about 2 billion yuan.

[0003] Long-term vaccine immunization and the interference of other diseases, pigs ar...

Claims

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