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A method of extracting microbial oil

A technology for microbial oil and oil-producing microorganisms, which is applied in the production of fat, fat oil/fat, etc., can solve the problems of high puffing temperature, high energy consumption and high requirements, and achieve the effect of efficient extraction, guaranteeing oil quality and saving energy consumption.

Inactive Publication Date: 2017-08-25
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has the advantages of simple process, large production capacity, high quality algae oil and high utilization rate of algae meal; the disadvantages are high puffing temperature and high energy consumption
The invention has the advantages of simplifying the extraction and demulsification process, reducing equipment investment, avoiding the burden of water treatment, and reducing costs; the disadvantage is that low pH values ​​consume a lot of acid, and the requirements for equipment are also high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh Chlorella with a wet weight of 2.3g (water content is 87.0%, initial total lipid content is 24.2%), add 8mL of n-hexane and analytical ethanol mixture (volume ratio is 1:2.2), 30 ℃, 220rpm After shaking and extracting for 3 hours, centrifuge at 8000 rpm for 10 min to collect the oil-containing mixed solvent. 10 mL of 75% ethanol and n-hexane mixed solvent (volume ratio of 3:1) was added to the algal residue after one extraction, and after 2 hours of shaking at 30°C and 220 rpm, the mixture was centrifuged at 8000 rpm for 10 min to collect the oil-containing mixed solvent. 10 mL of 75% ethanol and n-hexane mixed solvent (volume ratio of 3:1) was added to the algal residue after the second extraction, and after 1 hour of shaking at 30°C and 220 rpm, the mixture was centrifuged at 8000 rpm for 10 min to collect the oil-containing mixed solvent. The three oil-containing mixed solvents were combined and desolvated, and the oil extraction rate was 96.8%.

Embodiment 2

[0027] Weigh Chlorella with a wet weight of 2.3g (water content is 87.0%, initial total lipid content is 24.2%), add 8mL of n-hexane and analytical ethanol mixture (volume ratio is 1:2.2), 30 ℃, 220rpm After shaking and extracting for 3 hours, centrifuge at 8000 rpm for 10 min to collect the oil-containing mixed solvent. 10 mL of 75% ethanol and n-hexane mixed solvent (volume ratio of 3:1) was added to the algal residue after one extraction, and after 2 hours of shaking at 30°C and 220 rpm, the mixture was centrifuged at 8000 rpm for 10 min to collect the oil-containing mixed solvent. 10 mL of 75% ethanol and n-hexane mixed solvent (volume ratio of 3:1) was added to the algal residue after the second extraction, and after 30° C., 220 rpm shaking and extraction for 0.5 hours, centrifugation at 8000 rpm for 10 min to collect the oil-containing mixed solvent. After combining the three oil-containing mixed solvents and desolventizing, the oil extraction rate was 92.3%.

Embodiment 3

[0029] Weigh Chlorella with a wet weight of 1.55g (water content is 80.6%, initial total lipid content is 24.2%), add 5.75mL of n-hexane and analytical ethanol mixture (volume ratio is 1:2.3), 30 ℃, After shaking at 220 rpm for 3 hours, the mixture was centrifuged at 8000 rpm for 10 min to collect the oil-containing mixed solvent. 7 mL of 75% ethanol and n-hexane mixed solvent (volume ratio of 3:1) was added to the algal residue after one extraction, and after 2 hours of shaking at 30°C and 220 rpm, the mixture was centrifuged at 8000 rpm for 10 min to collect the oil-containing mixed solvent. 7 mL of 75% ethanol and n-hexane mixed solvent (volume ratio of 3:1) was added to the algal residue after the second extraction, and after 30° C., 220 rpm shaking extraction for 1 hour, centrifugation at 8000 rpm for 10 min, the oil-containing mixed solvent was collected. After combining the three oil-containing mixed solvents and desolventizing, the oil extraction rate was 80.8%.

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Abstract

The invention discloses a method for gently and efficiently extracting microbial oil, belonging to the field of grease extracting. The method comprises the following steps: enabling fermented grease-producing microorganisms to pass through a separating system to obtain wet thallus with moisture content of 65%-90%; adding a mixed solvent of normal hexane and alcohol into the wet thallus, and extracting for a certain time at a constant temperature; collecting an oil-containing mixed solvent a after solid-liquid separation, adding normal hexane and alcohol with certain concentration into thallus meal, and extracting for a certain time at a content temperature; collecting an oil-containing mixed solvent b after solid-liquid separation, and combining the oil-containing mixed solvents a and b; desolventizing the mixed grease to obtain microbial oil. According to the method disclosed by the invention, efficient extraction of the microbial oil can be realized by utilizing a low-toxicity organic solvent mixture; the extracting process does not need heating, so that the oil quality is guaranteed while the energy consumption is reduced; the process is simple, and large-scale production is easy to realize.

Description

technical field [0001] The invention belongs to the field of oil and fat extraction, and relates to a method for gently and efficiently extracting oil and fat from oil-producing microorganisms, in particular to a method for extracting oil and fat with high efficiency, low energy consumption, simple process, low cost and easy industrialization. Background technique [0002] Climate change and rising fossil fuel prices make research on sustainable and renewable energy increasingly important. Oil-producing microorganisms are considered to be the most promising alternative energy sources for biodiesel production due to their own series of advantages. Common oil-producing microorganisms usually include bacteria, yeast, mold, and microalgae. As far as microalgae are concerned, they have a short growth cycle and rich oil content, which can generally reach 20% to 50% of the dry weight of the cells, and the oil production per unit area can be dozens of times higher than that of oil ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/10
CPCC11B1/10
Inventor 张栩李雅赵文欢谭天伟
Owner BEIJING UNIV OF CHEM TECH