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Preparation method of mulberry leaf protein powder

A technology of protein powder and mulberry leaf powder, which is applied in food preparation, vegetable protein processing, protein food ingredients and other directions, can solve the problems of low protein content and poor solubility of leaf protein.

Active Publication Date: 2015-04-29
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the protein content of leaf protein extracted by various methods is not high and the solubility is poor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of mulberry leaf protein powder, is characterized in that, comprises the steps:

[0021] (1) Raw material pretreatment: collect fresh mulberry leaves, remove withered, yellowed, rotten leaves and leaves with pests and diseases in the mulberry leaves; wash and dry the selected mulberry leaves and coarsely pulverize them to obtain mulberry leaf powder;

[0022] (2) Superfine grinding: the mulberry leaf powder is ultrafinely pulverized to 200 orders to obtain the mulberry leaf powder;

[0023] (3) Extrusion and expansion: adjust the moisture content of the mulberry leaf micropowder to 12%, and adopt twin-screw extrusion under the conditions of extrusion temperature 80°C and screw speed 300rpm;

[0024] (4) Enzymatic hydrolysis and extraction: move the expanded mulberry leaf powder into the extraction tank, add 100U / g mulberry leaf powder cellulase, and use an acidic aqueous solution with a pH value of 5.5 at 50°C at a solid-liquid mass ratio of 1:10 A...

Embodiment 2

[0030] A preparation method of mulberry leaf protein powder, is characterized in that, comprises the steps:

[0031] (1) Raw material pretreatment: collect fresh mulberry leaves, remove withered, yellowed, rotten leaves and leaves with pests and diseases in the mulberry leaves; wash and dry the selected mulberry leaves and coarsely pulverize them to obtain mulberry leaf powder;

[0032] (2) Ultrafine grinding: the mulberry leaf powder is ultrafinely pulverized to 300 mesh to obtain the mulberry leaf powder;

[0033] (3) extrusion expansion: adjust the moisture content of mulberry leaf micropowder to 10%, and adopt twin-screw extrusion expansion under the conditions of extrusion temperature 70°C and screw speed 200rpm;

[0034] (4) Enzymatic hydrolysis and extraction: move the expanded mulberry leaf powder into the extraction tank, add 40U / g mulberry leaf powder cellulase, and use an acidic aqueous solution with a pH value of 5.5 at 50°C at a solid-liquid mass ratio of 1:5 Aft...

Embodiment 3

[0040] A preparation method of mulberry leaf protein powder, is characterized in that, comprises the steps:

[0041] (1) Raw material pretreatment: collect fresh mulberry leaves, remove withered, yellowed, rotten leaves and leaves with pests and diseases in the mulberry leaves; wash and dry the selected mulberry leaves and coarsely pulverize them to obtain mulberry leaf powder;

[0042] (2) Superfine grinding: the mulberry leaf powder is ultrafinely pulverized to 200 orders to obtain the mulberry leaf powder;

[0043] (3) extrusion puffing: adjust the moisture content of mulberry leaf powder to 12%, and adopt twin-screw extrusion puffing under the conditions of extrusion temperature 80°C and screw speed 250rpm;

[0044] (4) Enzymatic hydrolysis and extraction: move the expanded mulberry leaf powder into the extraction tank, add 30U / g mulberry leaf powder cellulase, and use an acidic aqueous solution with a pH value of 5.5 at 50°C at a solid-liquid mass ratio of 1:7 After 30 m...

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PUM

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Abstract

The invention belongs to the technical field of food, and in particular to a preparation method of mulberry leaf protein powder. The preparation method comprises the following steps: raw material pretreatment, superfine grinding, extruding for swelling, enzymatic extraction, alkali dissolution on protein, acid precipitation on protein, enzymolysis solubilizing, and drying to obtain mulberry leaf protein powder with high solubleness. According the preparation method, superfine grinding is combined with the extruding for the swelling, and is assisted by enzymolysis wall-breaking and enzymolysis solubilizing, so that the extraction rate of the protein is increased; the solubleness of the protein is greatly improved; the extraction rate of the protein is over 70%; the content of the protein in the obtained mulberry leaf protein powder is over 75%; the protein is good in solubleness; the nitrogen solubility index (NSI) is over 90%; and the mulberry leaf protein powder obtained by adopting the process disclosed by the invention is good in sensory quality, high in protein content, and good in solubleness, and can be used as a functional food material.

Description

technical field [0001] The invention belongs to the technical field of food, and in particular relates to a preparation method of mulberry leaf protein powder. Background technique [0002] Leaf protein is a protein concentrate prepared by dissolving, precipitating and drying the leaves of green plants. Leaf protein has a wide range of sources, is rich in nutrition, does not contain animal cholesterol, and has various physiological functions such as disease prevention, treatment, and anti-aging. It is a new type of protein resource with high development value. [0003] Mulberry leaves are the leaves of the genus Moraceae, and are one of the plant materials that have the same source of medicine and food and can be used for health food announced by the Ministry of Health. Mulberry leaves have a complete range of amino acids, and are high in essential amino acids and semi-essential amino acids. They are a high-quality protein resource. Traditionally, mulberry leaves are only ...

Claims

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Application Information

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IPC IPC(8): A23L1/00A23L3/14A23L5/00A23L33/185
CPCA23J1/007A23J3/14
Inventor 陈祥贵朱天明开拓杨潇杨文宇
Owner XIHUA UNIV
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