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Method for improving hydrophily and flexibility of polypeptide membrane by polycarbamate and polyethylene glycol

A technology of polyurethane and polyethylene glycol is applied in the field of polymer film preparation to achieve the effect of simple operation and easy mastery

Inactive Publication Date: 2015-04-29
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the improvement of the hydrophilicity and flexibility of the polypeptide film with polyurethane and polyethylene glycol.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Synthesis of Polyurethanes Containing Terminal -COOH Groups

[0020] Add 5.5 grams of 2,4-toluene diisocyanate and 17 grams of polytetrahydrofuran ether glycol (molecular weight: 1000) into the dry reactor, add 350 ml of dimethylformamide solvent, and add di Dibutyltin laurate, under an inert atmosphere, stirred and reacted at 40°C for 40 minutes, then added 0.8 g of 1,4-butanediol and reacted for 10 minutes, then added 1.2 g of 3-hydroxypropionic acid and reacted for 5 minutes to terminate the reaction and obtain the target thing;

[0021] 2) Synthesis of Polypeptide-Polyurethane Block Copolymer

[0022] Add 16 g of poly(r-benzyl-L-glutamate) (molecular weight 80,000) and 9 g of polyurethane containing terminal -COOH groups into a dry reactor, add 300 ml of dimethyl formamide, add another 0.045 g N,N' -Dicyclohexylcarbodiimide, under an inert atmosphere, stirred and reacted at 20°C for 3 days, then terminated the reaction, filtered, dialyzed, and dried to obtain ...

Embodiment 2

[0029] 1) Synthesis of Polyurethanes Containing Terminal -COOH Groups

[0030] Add 6.05 grams of 2,4-toluene diisocyanate and 19 grams of polytetrahydrofuran ether glycol (molecular weight: 1000) into the dry reactor, add 353 ml of dimethylformamide solvent, and add di Dibutyltin laurate, under an inert atmosphere, stirred and reacted at 45°C for 45 minutes, then added 0.88 g of 1,4-butanediol and reacted for 12 minutes, then added 1.3 g of 3-hydroxypropionic acid and reacted for 7 minutes to terminate the reaction and obtain the target thing;

[0031] 2) Synthesis of Polypeptide-Polyurethane Block Copolymer

[0032] Add 16.5 g of poly(r-ethyl-L-glutamate) (molecular weight: 85,000) and 9 g of polyurethane containing terminal -COOH groups into the dry reactor, add 310 ml of dimethylformaldehyde amide, add 0.027 g N,N' -Diisopropylcarbodiimide, under an inert atmosphere, stirred at 25°C for 4 days, then terminated the reaction, filtered, dialyzed, and dried to obtain the tar...

Embodiment 3

[0039] 1) Synthesis of Polyurethanes Containing Terminal -COOH Groups

[0040]Add 6.6 grams of 2,4-toluene diisocyanate, 21 grams of polytetrahydrofuran ether glycol (molecular weight: 1000) into the dry reactor, add 360 ml of dimethylformamide solvent, and add di Dibutyltin laurate, under an inert atmosphere, stirred and reacted at 50°C for 50 minutes, then added 0.92 g of 1,4-butanediol to react for 15 minutes, then added 1.84 g of 3-hydroxypropionic acid to react for 10 minutes, terminated the reaction, and obtained the target thing;

[0041] 2) Synthesis of Polypeptide-Polyurethane Block Copolymer

[0042] Add 16.8 g of poly(r-methyl-L-glutamate) (molecular weight: 90,000) and 9.9 g of polyurethane containing terminal -COOH groups into the dry reactor, add 315 ml of dimethylformaldehyde Amide, then add 0.046 g of 3-ethyl-1-(3-dimethylaminopropyl) carbodiimide, under an inert atmosphere, stir and react at 30°C for 3 days, then terminate the reaction, filter, dialyze, and ...

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PUM

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Abstract

The invention discloses a method for improving the hydrophily and flexibility of a polypeptide film by polycarbamate and polyethylene glycol. The method comprises the following steps: (1) adding diisocyanate, polytetrahydrofuran, a catalyst and a solvent into a reactor, adding butanediol to react for a period, and adding 3-hydroxypropionic acid to react to obtain carboxyl-terminated polycarbamate; (2) adding the carboxyl-terminated polycarbamate, a solvent, a condensing agent and polypeptide homopolymer into a reactor, and stirring to react for 3-4 days to obtain a polypeptide-polycarbamate copolymer; (3) adding the carboxyl-terminated polycarbamate, a solvent, a condensing agent and amino-terminated methoxy polyethylene glycol into a reactor, and reacting for 2-3 days to obtain a polyethylene glycol-polycarbamate copolymer; (4) adding the polypeptide-polycarbamate copolymer, the polyethylene glycol-polycarbamate copolymer and a solvent into a reactor, mixing for a period, forming a film by a tape casting method, and drying to obtain the target object. The preparation process is simple, and the hydrophily and flexibility of the modified film can be greatly improved.

Description

technical field [0001] The invention relates to a method for improving the hydrophilicity and flexibility of a polypeptide film with polyurethane and polyethylene glycol, and belongs to the technical field of polymer film preparation. Background technique [0002] Polypeptide is a biomaterial with good biocompatibility and biodegradability. Polypeptide film can be used as artificial skin, etc., but the polypeptide film is relatively stiff and lacks hydrophilicity, which limits its application. Polyurethane has better biocompatibility and biodegradability, and is relatively soft. Polyethylene glycol has good biocompatibility and biodegradability, is relatively soft and has good hydrophilicity. First, the polyurethane segment is introduced into the polypeptide segment and the polyethylene glycol segment to form a polypeptide-polyurethane block copolymer and a polyethylene glycol-polyurethane block copolymer , and then mix the two copolymers to form a blend with better comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L87/00C08J5/18C08G81/00C08G18/66C08G18/48C08G18/32C08G18/34
Inventor 朱国全王发刚柳玉英
Owner SHANDONG UNIV OF TECH