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SNP298299 marker significantly correlated with pinctada martensii mollusc part weight and adductor muscle weight, as well as primers and application of SNP298299 marker

A technology of 298299RI and 298299FI, which is applied in the field of aquatic animal genetics and molecular marker-assisted breeding, can solve the problems of the degeneration of the broodstock, the deterioration of the breeding environment, and the slow growth.

Active Publication Date: 2015-04-29
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In terms of the reasons, apart from the deteriorating breeding environment and backward breeding technology, there is another important reason: no attention has been paid to the selection and preservation of seed oysters for many years, and there is a lack of good varieties; , such as slow growth, small size, weakened pearl breeding ability, etc.

Method used

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  • SNP298299 marker significantly correlated with pinctada martensii mollusc part weight and adductor muscle weight, as well as primers and application of SNP298299 marker
  • SNP298299 marker significantly correlated with pinctada martensii mollusc part weight and adductor muscle weight, as well as primers and application of SNP298299 marker
  • SNP298299 marker significantly correlated with pinctada martensii mollusc part weight and adductor muscle weight, as well as primers and application of SNP298299 marker

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Embodiment 1

[0025] Randomly take 96 Pinctada martensii individuals from the same group. After cleaning, take their soft parts and weigh them, then take their adductor muscle tissues and weigh them and record them one by one. Take a small piece of adductor muscle Tissues were stored in 95% ethanol at -20°C. HiPure Universal DNA Kit (Guangzhou Meiji Biological Co., Ltd.) was used to extract the genomic DNA from the adductor muscle of 96 Pinctada martensii individuals. For related operations, refer to the kit instructions. After the DNA was extracted, its integrity was detected by electrophoresis on 1.0% agarose gel, and the concentration and purity of the DNA were measured by an ultraviolet spectrophotometer, and stored at -20°C.

[0026] The detection primers for the SNP298299 marker associated with the soft body weight and adductor muscle weight of Pinctada martensii are:

[0027] 298299FI:GGAATTCGTATCACTGTTTGAAGCAAGG;

[0028] 298299RI:GGGAACACATCCCACATTTAACAATGTTA;

[0029] 298299FO:...

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Abstract

The invention discloses an SNP298299 marker significantly correlated with a pinctada martensii mollusc part weight and an adductor muscle weight, as well as and primers and application of the SNP298299 marker. The primers comprise 298299FI: GGAATTCGTATCACTGTTTGAAGCAAGG, 298299RI: GGGAACACATCCCACATTTAACAATGTTA, 298299FO: TTGATAGCGGAATAGGCACATTCAATTT, and 298299RO: TTAAAAGGCAAAGAAAACTTTGGCATCAG. Two types of SNP (single nucleotide polymorphism) genotype loci can be amplified by utilizing the primers disclosed by the invention and then according to a PCR (Polymerase Chain Reaction) electrophoretogram, the mollusc part weight and the adductor muscle weight can be visually compared between individuals; and the primers can be directly used for subsequent molecular marker assistant breeding.

Description

Technical field: [0001] The invention belongs to the technical field of aquatic animal genetics and molecular marker-assisted breeding, and specifically relates to a SNP298299 marker, a primer and an application thereof that are significantly correlated with the soft body weight and adductor muscle weight of Pinctada martensii. Background technique: [0002] Pinctada martensii is the main species of pearl culture in my country and the world. Since the successful artificial breeding of Pinctada martensii in my country in 1965, the seawater pearl industry has developed rapidly and has become one of the pillar industries of mariculture in Guangdong, Guangxi and some coastal areas of Hainan. In the past 10 years, the quality and output of seawater pearls in my country have declined significantly, the international competitiveness has weakened, and the economic benefits of breeding have declined. In terms of the reasons, apart from the deteriorating breeding environment and back...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 何毛贤李耀国
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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